利用 HPLC/UV-ESI-MS 鉴定小叶牛至和长叶牛至中的化合物,并比较分析其抗氧化、抗菌、抗凝血和抗糖尿病特性

IF 3 3区 医学 Q2 PHARMACOLOGY & PHARMACY Saudi Pharmaceutical Journal Pub Date : 2024-10-02 DOI:10.1016/j.jsps.2024.102184
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The phytochemical examination of <em>O. compactum</em> revealed that lithospermic acid accounted for 36.82 % of the chemicals detected, followed by salvianolic acid C at 12.57 % and ros-marinic acid at 6.01 %. The main constituents of <em>O. elongatum</em> are salvianolic acid C (14.46 %), luteolin-3-O-glucuronide (13.51 %), salvianolic acid B (12.24 %), rosmarinic acid (7.83 %), and rutin (6.18 %). The results demonstrated different levels of effectiveness against the investigated microorganisms, with the extract from <em>O. compactum</em> exhibiting better activity, particularly against Gram-negative bacteria, certain yeasts, and the fungus Aspergillus niger. The aqueous extracts of both <em>Origanum</em> species demonstrate significant antioxidant activity. <em>O. compactum</em> has a higher total antioxidant capacity (IC<sub>50</sub> of 35.083 μg/mL) compared to <em>O. elongatum</em> (IC<sub>50</sub> of 77.080 μg/mL). 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引用次数: 0

摘要

目的是评估小叶牛至和长叶牛至的植物化学成分、酚类成分水平和生物特性。研究采用了植物化学测定法、分光光度法对多酚、类黄酮和单宁进行定量分析,并使用 HPLC/UV-ESI-MS 对化合物进行鉴定。此外,还对其抗菌、抗氧化、抗凝血和抗糖尿病特性进行了体外和体内检测。结果表明,O. compactum 提取物的总多酚含量很高,每克提取物中含有 47.368 毫克没食子酸当量,黄酮类化合物含量也很高,每克提取物中含有 14.839 毫克槲皮素当量。植物化学检查显示,在 O. compactum 检测到的化学物质中,石蒜酸占 36.82%,其次是丹酚酸 C(12.57%)和玫瑰橘酸(6.01%)。O. elongatum 的主要成分是丹酚酸 C(14.46 %)、木犀草素-3-O-葡萄糖醛酸苷(13.51 %)、丹酚酸 B(12.24 %)、迷迭香酸(7.83 %)和芦丁(6.18 %)。结果表明,O. compactum 的提取物对所研究的微生物具有不同程度的功效,尤其是对革兰氏阴性菌、某些酵母菌和黑曲霉菌具有更好的活性。两种牛至的水提取物都具有显著的抗氧化活性。与 O. elongatum(IC50 为 77.080 μg/mL)相比,O. compactum 的总抗氧化能力更高(IC50 为 35.083 μg/mL)。然而,与 O. compactum(42.563 μg/mL)相比,O. elongatum 的还原能力更高(35.697 μg/mL)。体内评估显示,O. compactum 和 O. elongatum 的水提取物具有显著的降血糖和抗凝特性。在对 Wistar 大鼠进行口服葡萄糖耐量试验(OGTT)时,这两种提取物能明显降低血糖水平,并能有效延长凝血酶原时间(PT)和活化部分凝血活酶时间(aPTT),突出了它们抑制凝血途径的能力。此外,它们的疗效与标准降糖药物相当,即使在高剂量下也没有严重毒性,这突出表明它们具有安全有效的治疗应用潜力。在这两个物种中,O. compactum 在抗氧化、抗菌和抗凝特性等关键生物活性方面表现出更高的功效,使其成为治疗应用的有力候选者。这项研究强调了牛至物种作为生物活性化合物丰富来源的价值,为制药、营养保健品和农业食品行业提供了巨大的潜力。研究结果为进一步探索牛至的多样化应用铺平了道路。
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Identification of compounds from Origanum compactum and Origanum elongatum using HPLC/UV-ESI-MS and comparative analysis of their antioxidant, antimicrobial, anticoagulant, and antidiabetic properties
The aim was to assess the phytochemical composition, phenolic component levels, and biological properties of the flowering tops of Origanum compactum and Origanum elongatum. The study employed phytochemical assays, spectrophotometric techniques for quantitative analysis of polyphenols, flavonoids, and tannins, and compound identification using HPLC/UV-ESI-MS. The antimicrobial, antioxidant, anticoagulant, and antidiabetic properties were examined both in vitro and in vivo. The results showed that the O. compactum extract had significantly high levels of total polyphenols, measuring 47.368 mg gallic acid equivalents per gram, and flavonoids, measuring 14.839 mg quercetin equivalents per gram. The phytochemical examination of O. compactum revealed that lithospermic acid accounted for 36.82 % of the chemicals detected, followed by salvianolic acid C at 12.57 % and ros-marinic acid at 6.01 %. The main constituents of O. elongatum are salvianolic acid C (14.46 %), luteolin-3-O-glucuronide (13.51 %), salvianolic acid B (12.24 %), rosmarinic acid (7.83 %), and rutin (6.18 %). The results demonstrated different levels of effectiveness against the investigated microorganisms, with the extract from O. compactum exhibiting better activity, particularly against Gram-negative bacteria, certain yeasts, and the fungus Aspergillus niger. The aqueous extracts of both Origanum species demonstrate significant antioxidant activity. O. compactum has a higher total antioxidant capacity (IC50 of 35.083 μg/mL) compared to O. elongatum (IC50 of 77.080 μg/mL). However, O. elongatum has a higher reducing power (35.697 μg/mL) compared to O. compactum (42.563 μg/mL). In vivo evaluations revealed that the aqueous extracts of O. compactum and O. elongatum possess significant antihyperglycemic and anticoagulant properties. The extracts demonstrated a marked reduction in blood glucose levels during the oral glucose tolerance test (OGTT) in Wistar rats and effectively prolonged both prothrombin time (PT) and activated partial thromboplastin time (aPTT), highlighting their ability to inhibit coagulation pathways. Moreover, their comparable efficacy to standard antihyperglycemic medications and absence of severe toxicity, even at high doses, underscore their therapeutic potential for safe and effective treatment applications. Between the two species, O. compactum exhibited superior efficacy in key biological activities such as antioxidant, antimicrobial, and anticoagulant properties, making it a strong candidate for therapeutic applications. This study underscores the value of Origanum species as a rich source of bioactive compounds, offering significant potential in pharmaceuticals, nutraceuticals, and agri-food industries. The findings pave the way for further exploration of their diverse applications.
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来源期刊
Saudi Pharmaceutical Journal
Saudi Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
6.10
自引率
2.40%
发文量
194
审稿时长
67 days
期刊介绍: The Saudi Pharmaceutical Journal (SPJ) is the official journal of the Saudi Pharmaceutical Society (SPS) publishing high quality clinically oriented submissions which encompass the various disciplines of pharmaceutical sciences and related subjects. SPJ publishes 8 issues per year by the Saudi Pharmaceutical Society, with the cooperation of the College of Pharmacy, King Saud University.
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