具有广泛宿主范围的新型奇异变形杆菌噬菌体 PmP19 的特征和基因组分析

IF 2.5 4区医学 Q3 VIROLOGY Archives of Virology Pub Date : 2024-10-16 DOI:10.1007/s00705-024-06154-9

Shengyi Han, Shuping Li, Guoyuan Hu, Mengtong Lei, Chunhua Li, Licheng Xiao, Shengqing Li

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摘要

从一个农场的污水中分离出了一种溶菌变形杆菌噬菌体 PmP19。PmP19 的头部呈二十面体（直径为 60 ± 3 nm），尾部较短（长 15 ± 2 nm）。其基因组是一个长度为 44,305 bp 的线性双链 DNA 分子，平均 GC 含量为 51.93%，有 52 个推测的开放阅读框（ORF）。BLASTn 比较和系统进化分析表明，Pmp19 与克雷伯氏噬菌体 vB_KpnP_ZK1 关系密切。生物信息学分析表明，PmP19 属于噬菌体亚家族 Molineuxvirinae。

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Characterization and genomic analysis phage PmP19, a novel Proteus mirabilis phage with a broad host range

A lytic Proteus mirabilis phage, PmP19, was isolated from sewage on a farm. PmP19 has an icosahedral head (60 ± 3 nm in diameter) and a short tail (15 ± 2 nm long). Its genome, a linear, double-stranded DNA molecule 44,305 bp in length with an average GC content of 51.93%, has 52 putative open reading frames (ORFs). BLASTn comparisons and phylogenetic analysis revealed a close relationship between Pmp19 and Klebsiella phage vB_KpnP_ZK1. Bioinformatic analysis revealed that PmP19 belongs to the phage subfamily Molineuxvirinae.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.