Katia Suemi Gozi , Caroline Rodrigues da Silva , Marlon do Valle Barroso , Jessica Priscila Barboza , Juliana Regina Peiró , Jean-Yves Madec , Marisa Haenni , Luiz Claudio Nogueira Mendes , Mara Corrêa Lelles Nogueira , Tiago Casella
{"title":"饲养场羔羊中对扩展头孢菌素耐药的大肠埃希菌的传播和特征：一项为期两年的双群体研究","authors":"Katia Suemi Gozi , Caroline Rodrigues da Silva , Marlon do Valle Barroso , Jessica Priscila Barboza , Juliana Regina Peiró , Jean-Yves Madec , Marisa Haenni , Luiz Claudio Nogueira Mendes , Mara Corrêa Lelles Nogueira , Tiago Casella","doi":"10.1016/j.cimid.2024.102258","DOIUrl":null,"url":null,"abstract":"<div><div>Dissemination of antimicrobial resistance in food animals is a One Health concern, but sheep production has been overlooked. This study aimed to explore the dissemination of <em>Escherichia coli</em> resistant to extended-spectrum cephalosporins (ESC) in feedlot lambs. Two pens were sampled on two occasions, and carcasses and other mammals living around were also tested. <em>E. coli</em> were recovered and antibiotic resistance determined. <em>bla</em><sub>CTX-M/CMY</sub> genes and their genetic localization were characterized. Whole genome sequencing (WGS) was performed to confirm clonal relationship. The most prevalent ESC-resistance genes in the 108 <em>E. coli</em> isolates were <em>bla</em><sub>CTX-M-55</sub> (53.7 %), <em>bla</em><sub>CTX-M-2</sub> (14.8 %) and <em>bla</em><sub>CMY-2</sub> (13.9 %). Most <em>bla</em><sub>CTX-M-55</sub> genes were found on the chromosome, but IncA/C, IncHI1, IncHI2 and IncF plasmids were also identified. Genetic diversity was observed even though ST6448 was by far the most frequent ST. WGS analysis showed high similarity among isolates recovered from feedlot lambs, animals in the surroundings and lambs’ carcasses, proving the clonal and plasmidic dissemination.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"114 ","pages":"Article 102258"},"PeriodicalIF":2.0000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dissemination and characterization of Escherichia coli resistant to extended-cephalosporins in feedlot lambs: A two-year two-population study\",\"authors\":\"Katia Suemi Gozi , Caroline Rodrigues da Silva , Marlon do Valle Barroso , Jessica Priscila Barboza , Juliana Regina Peiró , Jean-Yves Madec , Marisa Haenni , Luiz Claudio Nogueira Mendes , Mara Corrêa Lelles Nogueira , Tiago Casella\",\"doi\":\"10.1016/j.cimid.2024.102258\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Dissemination of antimicrobial resistance in food animals is a One Health concern, but sheep production has been overlooked. This study aimed to explore the dissemination of <em>Escherichia coli</em> resistant to extended-spectrum cephalosporins (ESC) in feedlot lambs. Two pens were sampled on two occasions, and carcasses and other mammals living around were also tested. <em>E. coli</em> were recovered and antibiotic resistance determined. <em>bla</em><sub>CTX-M/CMY</sub> genes and their genetic localization were characterized. Whole genome sequencing (WGS) was performed to confirm clonal relationship. The most prevalent ESC-resistance genes in the 108 <em>E. coli</em> isolates were <em>bla</em><sub>CTX-M-55</sub> (53.7 %), <em>bla</em><sub>CTX-M-2</sub> (14.8 %) and <em>bla</em><sub>CMY-2</sub> (13.9 %). Most <em>bla</em><sub>CTX-M-55</sub> genes were found on the chromosome, but IncA/C, IncHI1, IncHI2 and IncF plasmids were also identified. Genetic diversity was observed even though ST6448 was by far the most frequent ST. WGS analysis showed high similarity among isolates recovered from feedlot lambs, animals in the surroundings and lambs’ carcasses, proving the clonal and plasmidic dissemination.</div></div>\",\"PeriodicalId\":50999,\"journal\":{\"name\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"volume\":\"114 \",\"pages\":\"Article 102258\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-10-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147957124001358\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Immunology Microbiology and Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147957124001358","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Dissemination and characterization of Escherichia coli resistant to extended-cephalosporins in feedlot lambs: A two-year two-population study
Dissemination of antimicrobial resistance in food animals is a One Health concern, but sheep production has been overlooked. This study aimed to explore the dissemination of Escherichia coli resistant to extended-spectrum cephalosporins (ESC) in feedlot lambs. Two pens were sampled on two occasions, and carcasses and other mammals living around were also tested. E. coli were recovered and antibiotic resistance determined. blaCTX-M/CMY genes and their genetic localization were characterized. Whole genome sequencing (WGS) was performed to confirm clonal relationship. The most prevalent ESC-resistance genes in the 108 E. coli isolates were blaCTX-M-55 (53.7 %), blaCTX-M-2 (14.8 %) and blaCMY-2 (13.9 %). Most blaCTX-M-55 genes were found on the chromosome, but IncA/C, IncHI1, IncHI2 and IncF plasmids were also identified. Genetic diversity was observed even though ST6448 was by far the most frequent ST. WGS analysis showed high similarity among isolates recovered from feedlot lambs, animals in the surroundings and lambs’ carcasses, proving the clonal and plasmidic dissemination.
期刊介绍：
Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine .
The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.
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