黄连水提取物的血液相容性和血液流变学活性:对体外糖化红细胞的影响

IF 2.2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Herbal Medicine Pub Date : 2024-09-19 DOI:10.1016/j.hermed.2024.100945
Hermano Mascaro Grosso , Patricia Buszniez , Bibiana D. Riquelme
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引用次数: 0

摘要

导言Phyllanthus sellowianus (Klotzsch) Müll Arg.是一种阿根廷和巴西本地植物,用于治疗糖尿病。在糖尿病等血管疾病中,红细胞会形成异常聚集,无法解离,这与高血糖水平有关。本研究旨在评估黄皮属植物水提取物的血液相容性和血液流变学活性,以及利用高血糖引起的体外糖化红细胞评估其抗糖尿病活性。以生理盐水为溶剂,对采集的树叶和树皮进行浸泡、浸渍、消化和烹饪提取。萃取物的渗透压和 pH 值经过调整,以便与人体细胞充分互动。使用健康捐献者(n=3)的红细胞(RBCs)并与葡萄糖溶液培养,以体外模拟高血糖效应。将红细胞与水提取物培养,以评估其血液相容性。此外,还将糖化的红细胞与提取物培养,以分析它们的抗糖尿病特性。红细胞流变仪和光学芯片聚集仪用于血液流变学评估。此外,在某些情况下,黄皮提取物逆转了体外糖化效应,血液流变学参数接近控制值。这些结果还提供了相关信息,有助于了解这些提取物或其成分在植物药中用作抗糖尿病药物的作用机制。
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Hemocompatibility and hemorheological activity of aqueous extracts from Phyllanthus sellowianus: effects on in vitro glycated erythrocytes

Introduction

Phyllanthus sellowianus (Klotzsch) Müll Arg. is an Argentinian and Brazilian native plant used to treat diabetes. In vascular diseases like diabetes, erythrocytes form anomalous aggregates resistant to dissociation, associated with high glucose levels. This work aims to evaluate the hemocompatibility and hemorheological activity of aqueous extracts from P. sellowianus and its anti-diabetic activity using in vitro glycated erythrocytes as occur by hyperglycemia.

Methods

For this work, P. sellowianus specimens were collected on the Nogoyá River coast, Argentina, and morphoanatomical identified. Infusion, maceration, digestion, and cooking extracts were prepared with collected leaves and bark using saline as solvent. The osmolality and pH of the extracts were adjusted to an adequate interaction with human cells. Red blood cells (RBCs) from healthy donors (n=3) were used and incubated with glucose solution for in vitro simulation of the hyperglycemia effects. RBCs were incubated with the aqueous extracts to assess their hemocompatibility. Also, glycated RBCs were incubated with the extracts to analyze their anti-diabetic properties. Erythrocyte Rheometer and Optical Chip Aggregameter were used for the hemorheological evaluation.

Results

Assays with the different P. sellowianus extracts modified the viscoelasticity and aggregation of RBCs. Moreover, in some cases, P. sellowianus extracts reversed the in vitro glycation effect and hemorheological parameters got nearer control values.

Conclusion

These results would be helpful for the development of pharmaceutical formulations using this species or its phytocomponents. They also give relevant information to understand the action mechanisms through which these extracts or their components can be used as anti-diabetics in Phytomedicine.
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来源期刊
Journal of Herbal Medicine
Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
3.90
自引率
0.00%
发文量
94
期刊介绍: The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.
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