Yuanquan Ran , Lanlan Yang , Xiaoyan Jia , Huan Zhao , Qiong Hu , Bing Yang , Dongxin Tang , Minyi Tian
{"title":"三叶金雀花种子的植物化学成分和对非小细胞肺癌 A549 细胞的抗癌作用","authors":"Yuanquan Ran , Lanlan Yang , Xiaoyan Jia , Huan Zhao , Qiong Hu , Bing Yang , Dongxin Tang , Minyi Tian","doi":"10.1016/j.arabjc.2024.106020","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Akebia trifoliata</em> is a widely distributed medicine and food homology plant. Its fruit is used to treat tumors and the seed’s weight proportion exceeds 50%. However, the anti-tumor effect of <em>A. trifoliata</em> seed remains poorly studied.</div></div><div><h3>Methods</h3><div>UHPLC-Q-Orbitrap-MS was used to identify the chemical components of <em>A. trifoliata</em> seed water extract (WE) and ethanolic extract (EE), and their cytotoxicity was evaluated using MTT assay. Further, colony formation and cell cycle assays examined the anti-proliferative effect of <em>A. trifoliata</em> seed EE. Next, morphology observation, AO/EB staining, Hoechst 33,258 staining, Annexin V-PE/7-AAD staining, and JC-1 assays were employed to detect apoptosis. The influence of <em>A. trifoliata</em> seed EE on A549 cell metastasis was assessed by wound healing and transwell invasion tests. In addition, western blotting and network pharmacology were applied to further analyze its anticancer mechanism.</div></div><div><h3>Results</h3><div>Eighty-two components were characterized from the <em>A. trifoliata</em> seed WE and EE. For anticancer activity, <em>A. trifoliata</em> seed EE exhibited high cytotoxicity against cancer cells A549 (IC<sub>50</sub>: 52.38 ± 1.04 μg/mL) and NCI-H1299 (IC<sub>50</sub>: 70.29 ± 0.58 μg/mL) and revealed low cytotoxicity against non-cancer cells MRC-5 (IC<sub>50</sub>: 101.28 ± 2.84 μg/mL) and L929 (IC<sub>50</sub>: 113.44 ± 0.55 μg/mL). <em>A. trifoliata</em> seed EE suppressed A549 cell proliferation by inducing S-phase arrest <em>via</em> down-regulation of CDK2 and p21 as well as up-regulation of cyclin E1 and E2. It increased the Bax/Bcl-2 ratio, reduced mitochondrial membrane potential (ΔΨm), activated caspase-9 and caspase-3, cleaved PARP, and triggered apoptosis through the mitochondrial pathway. Besides, it repressed migratory and invasive ability by reducing MMP-2, MMP-9, and N-cadherin levels.</div></div><div><h3>Conclusion</h3><div><em>A. trifoliata</em> seed EE exhibits outstanding anticancer properties and has the exploitation potential as an anticancer drug in the pharmaceutical industry.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106020"},"PeriodicalIF":5.3000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical composition and anticancer effect of Akebia trifoliata seed in non-small cell lung cancer A549 cells\",\"authors\":\"Yuanquan Ran , Lanlan Yang , Xiaoyan Jia , Huan Zhao , Qiong Hu , Bing Yang , Dongxin Tang , Minyi Tian\",\"doi\":\"10.1016/j.arabjc.2024.106020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div><em>Akebia trifoliata</em> is a widely distributed medicine and food homology plant. Its fruit is used to treat tumors and the seed’s weight proportion exceeds 50%. However, the anti-tumor effect of <em>A. trifoliata</em> seed remains poorly studied.</div></div><div><h3>Methods</h3><div>UHPLC-Q-Orbitrap-MS was used to identify the chemical components of <em>A. trifoliata</em> seed water extract (WE) and ethanolic extract (EE), and their cytotoxicity was evaluated using MTT assay. Further, colony formation and cell cycle assays examined the anti-proliferative effect of <em>A. trifoliata</em> seed EE. Next, morphology observation, AO/EB staining, Hoechst 33,258 staining, Annexin V-PE/7-AAD staining, and JC-1 assays were employed to detect apoptosis. The influence of <em>A. trifoliata</em> seed EE on A549 cell metastasis was assessed by wound healing and transwell invasion tests. In addition, western blotting and network pharmacology were applied to further analyze its anticancer mechanism.</div></div><div><h3>Results</h3><div>Eighty-two components were characterized from the <em>A. trifoliata</em> seed WE and EE. For anticancer activity, <em>A. trifoliata</em> seed EE exhibited high cytotoxicity against cancer cells A549 (IC<sub>50</sub>: 52.38 ± 1.04 μg/mL) and NCI-H1299 (IC<sub>50</sub>: 70.29 ± 0.58 μg/mL) and revealed low cytotoxicity against non-cancer cells MRC-5 (IC<sub>50</sub>: 101.28 ± 2.84 μg/mL) and L929 (IC<sub>50</sub>: 113.44 ± 0.55 μg/mL). <em>A. trifoliata</em> seed EE suppressed A549 cell proliferation by inducing S-phase arrest <em>via</em> down-regulation of CDK2 and p21 as well as up-regulation of cyclin E1 and E2. It increased the Bax/Bcl-2 ratio, reduced mitochondrial membrane potential (ΔΨm), activated caspase-9 and caspase-3, cleaved PARP, and triggered apoptosis through the mitochondrial pathway. Besides, it repressed migratory and invasive ability by reducing MMP-2, MMP-9, and N-cadherin levels.</div></div><div><h3>Conclusion</h3><div><em>A. trifoliata</em> seed EE exhibits outstanding anticancer properties and has the exploitation potential as an anticancer drug in the pharmaceutical industry.</div></div>\",\"PeriodicalId\":249,\"journal\":{\"name\":\"Arabian Journal of Chemistry\",\"volume\":\"17 12\",\"pages\":\"Article 106020\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Arabian Journal of Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1878535224004222\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arabian Journal of Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878535224004222","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Phytochemical composition and anticancer effect of Akebia trifoliata seed in non-small cell lung cancer A549 cells
Background
Akebia trifoliata is a widely distributed medicine and food homology plant. Its fruit is used to treat tumors and the seed’s weight proportion exceeds 50%. However, the anti-tumor effect of A. trifoliata seed remains poorly studied.
Methods
UHPLC-Q-Orbitrap-MS was used to identify the chemical components of A. trifoliata seed water extract (WE) and ethanolic extract (EE), and their cytotoxicity was evaluated using MTT assay. Further, colony formation and cell cycle assays examined the anti-proliferative effect of A. trifoliata seed EE. Next, morphology observation, AO/EB staining, Hoechst 33,258 staining, Annexin V-PE/7-AAD staining, and JC-1 assays were employed to detect apoptosis. The influence of A. trifoliata seed EE on A549 cell metastasis was assessed by wound healing and transwell invasion tests. In addition, western blotting and network pharmacology were applied to further analyze its anticancer mechanism.
Results
Eighty-two components were characterized from the A. trifoliata seed WE and EE. For anticancer activity, A. trifoliata seed EE exhibited high cytotoxicity against cancer cells A549 (IC50: 52.38 ± 1.04 μg/mL) and NCI-H1299 (IC50: 70.29 ± 0.58 μg/mL) and revealed low cytotoxicity against non-cancer cells MRC-5 (IC50: 101.28 ± 2.84 μg/mL) and L929 (IC50: 113.44 ± 0.55 μg/mL). A. trifoliata seed EE suppressed A549 cell proliferation by inducing S-phase arrest via down-regulation of CDK2 and p21 as well as up-regulation of cyclin E1 and E2. It increased the Bax/Bcl-2 ratio, reduced mitochondrial membrane potential (ΔΨm), activated caspase-9 and caspase-3, cleaved PARP, and triggered apoptosis through the mitochondrial pathway. Besides, it repressed migratory and invasive ability by reducing MMP-2, MMP-9, and N-cadherin levels.
Conclusion
A. trifoliata seed EE exhibits outstanding anticancer properties and has the exploitation potential as an anticancer drug in the pharmaceutical industry.
期刊介绍：
The Arabian Journal of Chemistry is an English language, peer-reviewed scholarly publication in the area of chemistry. The Arabian Journal of Chemistry publishes original papers, reviews and short reports on, but not limited to: inorganic, physical, organic, analytical and biochemistry.
The Arabian Journal of Chemistry is issued by the Arab Union of Chemists and is published by King Saud University together with the Saudi Chemical Society in collaboration with Elsevier and is edited by an international group of eminent researchers.
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