Lei Tian, Shadman Khan, Amid Shakeri, Kyle Jackson, Ahmed T. Saif, Fereshteh Bayat, Leon He, Jimmy Gu, Yingfu Li, Tohid F. Didar, Zeinab Hosseinidoust
{"title":"具有分层三维纳米网状网络的病毒组装生物功能微阵列","authors":"Lei Tian, Shadman Khan, Amid Shakeri, Kyle Jackson, Ahmed T. Saif, Fereshteh Bayat, Leon He, Jimmy Gu, Yingfu Li, Tohid F. Didar, Zeinab Hosseinidoust","doi":"10.1002/adfm.202414375","DOIUrl":null,"url":null,"abstract":"Three-dimensional (3D) hierarchical wrinkled materials built with biological entities have so far remained exclusive to nature. Herein, multiscale functional ultraporous 3D bio-networks of bioprinted phage-built wrinkled microarrays are created through establishing a universal heat- and solvent-independent substrate-shrinkage method induced by high-pressure carbon dioxide (HPCD). This method results in diverse wrinkled patterns on soft materials and is particularly powerful for solvent- and heat-sensitive biomaterials, for which other methods have failed. The phage nanofilaments (7 nm width) self-assemble into orderly-aligned submicron bundles (100 nm width), which crimp into tunable microscale wrinkles (0.7–5.0 µm width) on size-controllable micro-arrays (200–600 µm width) exhibiting a four-level hierarchical nano-reticular structure. The HPCD method also protects the bioactivity of biorecognition molecules loaded into the microarrays, leading to the design of bacteria-sensing chips, made with in-house deoxyribozyme-loaded 3D phage microarrays. The developed bacteria-sensing chips achieve a limit of detection that is 100 × more sensitive with greater reproducibility compared to two-dimensional (2D) microdot arrays and correctly identify <i>Legionella pneumophila</i> in contaminated water samples collected from industrial cooling towers, highlighting phage-built wrinkled networks as a platform for bottom-up assembly of biological building blocks into biofunctional material.","PeriodicalId":112,"journal":{"name":"Advanced Functional Materials","volume":null,"pages":null},"PeriodicalIF":18.5000,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Virus-Assembled Biofunctional Microarrays with Hierarchical 3D Nano-Reticular Network\",\"authors\":\"Lei Tian, Shadman Khan, Amid Shakeri, Kyle Jackson, Ahmed T. Saif, Fereshteh Bayat, Leon He, Jimmy Gu, Yingfu Li, Tohid F. 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The HPCD method also protects the bioactivity of biorecognition molecules loaded into the microarrays, leading to the design of bacteria-sensing chips, made with in-house deoxyribozyme-loaded 3D phage microarrays. 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Virus-Assembled Biofunctional Microarrays with Hierarchical 3D Nano-Reticular Network
Three-dimensional (3D) hierarchical wrinkled materials built with biological entities have so far remained exclusive to nature. Herein, multiscale functional ultraporous 3D bio-networks of bioprinted phage-built wrinkled microarrays are created through establishing a universal heat- and solvent-independent substrate-shrinkage method induced by high-pressure carbon dioxide (HPCD). This method results in diverse wrinkled patterns on soft materials and is particularly powerful for solvent- and heat-sensitive biomaterials, for which other methods have failed. The phage nanofilaments (7 nm width) self-assemble into orderly-aligned submicron bundles (100 nm width), which crimp into tunable microscale wrinkles (0.7–5.0 µm width) on size-controllable micro-arrays (200–600 µm width) exhibiting a four-level hierarchical nano-reticular structure. The HPCD method also protects the bioactivity of biorecognition molecules loaded into the microarrays, leading to the design of bacteria-sensing chips, made with in-house deoxyribozyme-loaded 3D phage microarrays. The developed bacteria-sensing chips achieve a limit of detection that is 100 × more sensitive with greater reproducibility compared to two-dimensional (2D) microdot arrays and correctly identify Legionella pneumophila in contaminated water samples collected from industrial cooling towers, highlighting phage-built wrinkled networks as a platform for bottom-up assembly of biological building blocks into biofunctional material.
期刊介绍:
Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week.
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