{"title":"通过盐退火† 在 PDADMAC-NaPSS 盐基塑料中实现溶菌酶功能化","authors":"Jiaying Li, Lijie Li and Saskia Lindhoud","doi":"10.1039/D4RA04986A","DOIUrl":null,"url":null,"abstract":"<p >Hot-pressed saloplastics are dense and transparent polyelectrolyte complex materials governed by ionic crosslinking. Such plastics have several advantages, for example, salt water processibility and recyclability. Here, we demonstrate a simple but effective post-treatment method to incorporate lysozyme as a biocatalytic component into the hot-pressed saloplastics. Changes in salt concentration can be used for annealing and curing the saloplastics, where the temporary opening allows for lysozyme loading. This process was carefully examined by two different routes and the salt concentrations and incubation times were varied systematically. Optimised saloplastics showed an enzymatic activity against <em>Micrococcus lysodeikticus</em> of 4.44 ± 0.37 U cm<small><sup>−2</sup></small> and remained partially active (∼72% activity preserved) after 7 days. This approach opens new routes to incorporate enzymes or other biological functionality into saloplastics which is difficult to achieve for conventional plastics.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/ra/d4ra04986a?page=search","citationCount":"0","resultStr":"{\"title\":\"Achieving lysozyme functionalization in PDADMAC–NaPSS saloplastics through salt annealing†\",\"authors\":\"Jiaying Li, Lijie Li and Saskia Lindhoud\",\"doi\":\"10.1039/D4RA04986A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Hot-pressed saloplastics are dense and transparent polyelectrolyte complex materials governed by ionic crosslinking. Such plastics have several advantages, for example, salt water processibility and recyclability. Here, we demonstrate a simple but effective post-treatment method to incorporate lysozyme as a biocatalytic component into the hot-pressed saloplastics. Changes in salt concentration can be used for annealing and curing the saloplastics, where the temporary opening allows for lysozyme loading. This process was carefully examined by two different routes and the salt concentrations and incubation times were varied systematically. Optimised saloplastics showed an enzymatic activity against <em>Micrococcus lysodeikticus</em> of 4.44 ± 0.37 U cm<small><sup>−2</sup></small> and remained partially active (∼72% activity preserved) after 7 days. This approach opens new routes to incorporate enzymes or other biological functionality into saloplastics which is difficult to achieve for conventional plastics.</p>\",\"PeriodicalId\":102,\"journal\":{\"name\":\"RSC Advances\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2024/ra/d4ra04986a?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Advances\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/ra/d4ra04986a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Advances","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/ra/d4ra04986a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
热压盐塑料是由离子交联而成的致密透明的聚电解质复合材料。这种塑料具有多种优点,例如盐水加工性和可回收性。在这里,我们展示了一种简单而有效的后处理方法,将溶菌酶作为生物催化成分加入热压盐塑料中。盐浓度的变化可用于盐基塑料的退火和固化,临时开口可使溶菌酶装入。我们通过两种不同的方法对这一过程进行了仔细研究,并系统地改变了盐浓度和培养时间。优化后的盐基塑料对溶血微球菌的酶活性为 4.44 ± 0.37 U cm-2,7 天后仍保持部分活性(活性保持率为 72%)。这种方法为在盐基塑料中加入酶或其他生物功能开辟了新途径,而传统塑料很难做到这一点。
Achieving lysozyme functionalization in PDADMAC–NaPSS saloplastics through salt annealing†
Hot-pressed saloplastics are dense and transparent polyelectrolyte complex materials governed by ionic crosslinking. Such plastics have several advantages, for example, salt water processibility and recyclability. Here, we demonstrate a simple but effective post-treatment method to incorporate lysozyme as a biocatalytic component into the hot-pressed saloplastics. Changes in salt concentration can be used for annealing and curing the saloplastics, where the temporary opening allows for lysozyme loading. This process was carefully examined by two different routes and the salt concentrations and incubation times were varied systematically. Optimised saloplastics showed an enzymatic activity against Micrococcus lysodeikticus of 4.44 ± 0.37 U cm−2 and remained partially active (∼72% activity preserved) after 7 days. This approach opens new routes to incorporate enzymes or other biological functionality into saloplastics which is difficult to achieve for conventional plastics.
期刊介绍:
An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.