Ya-Qian Wu , Xiang-Yu Wang , Tian-Wen Bai , Ying-Long Fu
{"title":"用于检测紫花苜蓿(Medicago sativa)中 SO2 和 Hg2+,以及 Cd2+ 条件下 SO2 动态成像的双通道荧光探针","authors":"Ya-Qian Wu , Xiang-Yu Wang , Tian-Wen Bai , Ying-Long Fu","doi":"10.1016/j.aca.2024.343353","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>As an antioxidant, SO<sub>2</sub> plays a critical role in maintaining the plant's redox balance. At the same time, Hg<sup>2+</sup> as heavy metals, will accumulate in plants through soil, often disrupting physiological processes in plants. However, few studies have been reported to examine changes in Hg<sup>2+</sup> and SO<sub>2</sub> in plants. Here, we developed a dual-channel fluorescent probe NYD, which can differentiate between the detection of Hg<sup>2+</sup> and SO<sub>2</sub>.</div></div><div><h3>Results</h3><div>The probe exhibits high sensitivity (LOD, 44 nM for Hg<sup>2+</sup> and 26 nM for SO<sub>2</sub>). When Hg<sup>2+</sup> and HSO<sub>3</sub><sup>−</sup> deprotect and attack the double bond by nucleophilic addition, significant emission of fluorescence at 475 nm and 574 nm with blue and red fluorescence respectively. In addition, NYD has the capability to detect the levels of Hg<sup>2+</sup> and SO<sub>2</sub> in cells and alfalfa seedlings. Meanwhile, the findings indicated that alfalfa seedlings were exposed to Cd<sup>2+</sup>, which led to redox imbalance and increased endogenous SO<sub>2</sub> production.</div></div><div><h3>Significance</h3><div>We anticipate that this research will contribute to a better understanding of the functions of SO<sub>2</sub> under oxidative stress induced by Cd<sup>2+</sup>.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343353"},"PeriodicalIF":5.7000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dual-channel fluorescent probe for detecting SO2 and Hg2+, and dynamic imaging SO2 under Cd2+ in alfalfa (Medicago sativa)\",\"authors\":\"Ya-Qian Wu , Xiang-Yu Wang , Tian-Wen Bai , Ying-Long Fu\",\"doi\":\"10.1016/j.aca.2024.343353\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>As an antioxidant, SO<sub>2</sub> plays a critical role in maintaining the plant's redox balance. At the same time, Hg<sup>2+</sup> as heavy metals, will accumulate in plants through soil, often disrupting physiological processes in plants. However, few studies have been reported to examine changes in Hg<sup>2+</sup> and SO<sub>2</sub> in plants. Here, we developed a dual-channel fluorescent probe NYD, which can differentiate between the detection of Hg<sup>2+</sup> and SO<sub>2</sub>.</div></div><div><h3>Results</h3><div>The probe exhibits high sensitivity (LOD, 44 nM for Hg<sup>2+</sup> and 26 nM for SO<sub>2</sub>). When Hg<sup>2+</sup> and HSO<sub>3</sub><sup>−</sup> deprotect and attack the double bond by nucleophilic addition, significant emission of fluorescence at 475 nm and 574 nm with blue and red fluorescence respectively. In addition, NYD has the capability to detect the levels of Hg<sup>2+</sup> and SO<sub>2</sub> in cells and alfalfa seedlings. Meanwhile, the findings indicated that alfalfa seedlings were exposed to Cd<sup>2+</sup>, which led to redox imbalance and increased endogenous SO<sub>2</sub> production.</div></div><div><h3>Significance</h3><div>We anticipate that this research will contribute to a better understanding of the functions of SO<sub>2</sub> under oxidative stress induced by Cd<sup>2+</sup>.</div></div>\",\"PeriodicalId\":240,\"journal\":{\"name\":\"Analytica Chimica Acta\",\"volume\":\"1332 \",\"pages\":\"Article 343353\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-10-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003267024011541\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003267024011541","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Dual-channel fluorescent probe for detecting SO2 and Hg2+, and dynamic imaging SO2 under Cd2+ in alfalfa (Medicago sativa)
Background
As an antioxidant, SO2 plays a critical role in maintaining the plant's redox balance. At the same time, Hg2+ as heavy metals, will accumulate in plants through soil, often disrupting physiological processes in plants. However, few studies have been reported to examine changes in Hg2+ and SO2 in plants. Here, we developed a dual-channel fluorescent probe NYD, which can differentiate between the detection of Hg2+ and SO2.
Results
The probe exhibits high sensitivity (LOD, 44 nM for Hg2+ and 26 nM for SO2). When Hg2+ and HSO3− deprotect and attack the double bond by nucleophilic addition, significant emission of fluorescence at 475 nm and 574 nm with blue and red fluorescence respectively. In addition, NYD has the capability to detect the levels of Hg2+ and SO2 in cells and alfalfa seedlings. Meanwhile, the findings indicated that alfalfa seedlings were exposed to Cd2+, which led to redox imbalance and increased endogenous SO2 production.
Significance
We anticipate that this research will contribute to a better understanding of the functions of SO2 under oxidative stress induced by Cd2+.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.