从结构上洞察细菌同源重组过程中 DNA 分支迁移的机制。

Leonardo Talachia Rosa,Émeline Vernhes,Anne-Lise Soulet,Patrice Polard,Rémi Fronzes
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引用次数: 0

摘要

一些 DNA 螺旋酶通过在同源重组的不同途径中的分支迁移活动,在基因组的维护和可塑性方面发挥着核心和特殊的作用。RadA 是一种高度保守的细菌螺旋酶,参与所有细菌物种的 DNA 修复。在革兰氏阳性真菌中,它还在自然转化中发挥作用,而在革兰氏阴性细菌中,ComM 是典型的转化特异性螺旋酶。RadA 和 ComM 螺旋酶都形成六聚体环,并利用 ATP 水解作为能量来源,推动自身沿着 DNA 运行。在这项研究中,我们展示了 RadA 和 ComM 与 DNA 和 ATP 类似物相互作用的冷冻电镜结构。这些结构揭示了 RadA 中 ATP 水解与 DNA 结合之间的重要分子相互作用,以及 RadA 和 ComM 共享的 Lon 蛋白酶样结构域在这一过程中的作用。总之,这些结果为了解同源重组不同途径中 DNA 分支迁移的机制提供了新的分子见解。
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Structural insights into the mechanism of DNA branch migration during homologous recombination in bacteria.
Some DNA helicases play central and specific roles in genome maintenance and plasticity through their branch migration activity in different pathways of homologous recombination. RadA is a highly conserved bacterial helicase involved in DNA repair throughout all bacterial species. In Gram-positive Firmicutes, it also has a role in natural transformation, while in Gram-negative bacteria, ComM is the canonical transformation-specific helicase. Both RadA and ComM helicases form hexameric rings and use ATP hydrolysis as an energy source to propel themselves along DNA. In this study, we present the cryoEM structures of RadA and ComM interacting with DNA and ATP analogs. These structures reveal important molecular interactions that couple ATP hydrolysis and DNA binding in RadA, as well as the role of the Lon protease-like domain, shared by RadA and ComM, in this process. Taken together, these results provide new molecular insights into the mechanisms of DNA branch migration in different pathways of homologous recombination.
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