Lei Li, Lezhu Su, Jun Gao, Shiyu Liu, Shijie Yuan, Nan Zhou, Zhi Zhou, Dongdong Wang, Yan Zhou, Xiaohu Dai
{"title":"在微生物电解池中强化生物膜对废弃活性污泥发酵液的沼气提升作用","authors":"Lei Li, Lezhu Su, Jun Gao, Shiyu Liu, Shijie Yuan, Nan Zhou, Zhi Zhou, Dongdong Wang, Yan Zhou, Xiaohu Dai","doi":"10.1016/j.watres.2024.122675","DOIUrl":null,"url":null,"abstract":"This study demonstrated that metal-organic frameworks (MOFs)-derived Fe-NC cathode improved both methane yield and methane content in a microbial electrolysis cell-coupled anaerobic digestion (MEC-AD) system treating waste activated sludge (WAS) fermentation liquor. Results revealed that Fe-NC maintained a meso-macroporous structure with a large specific surface area of 1381 m<sup>2</sup>/g and superior electrochemical properties. Its calculated specific capacitance and electron transfer resistance were 5.7 and 0.4 times of the carbon felt (CF) group. The bacterial and archaeal gene loads of Fe-NC biofilm after multiple acclimation cycles were 5.69E+10 and 1.86E+9 copies/cm<sup>2</sup> and <em>Proteiniphilum</em> and <em>Methanobacterium</em> were the most enriched syntrophs from stage Ⅰ to stage Ⅱ acclimation. Corresponding maximum methane yield and content achieved were 0.31 m<sup>3</sup> CH<sub>4</sub>/kg COD and 92.8%, and its CO<sub>2</sub>-dependent methane production was improved by 107.6%. Mechanistic investigations showed that Fe-NC biofilm improved enzyme-associated CO<sub>2</sub> reduction pathway companying by promoting the intra- and extracellular electron transfer as well as ATP synthesis, therefore favoring methanogenic energetic metabolism. More importantly, an enhanced proton-coupled electron transfer (PCET) process was proposed within Fe-NC biofilm, providing a synergistic advantage over unbalanced conventional sole electron/proton transfer. This work provides an effective strategy to strengthen the waste-to-energy and biogas upgrading technology, potentially bringing economic benefits to wastewater treatment.","PeriodicalId":443,"journal":{"name":"Water Research","volume":null,"pages":null},"PeriodicalIF":11.4000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced Biofilm-dependent Biogas Upgrading from Waste Activated Sludge Fermentation Liquor in Microbial Electrolysis Cells\",\"authors\":\"Lei Li, Lezhu Su, Jun Gao, Shiyu Liu, Shijie Yuan, Nan Zhou, Zhi Zhou, Dongdong Wang, Yan Zhou, Xiaohu Dai\",\"doi\":\"10.1016/j.watres.2024.122675\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study demonstrated that metal-organic frameworks (MOFs)-derived Fe-NC cathode improved both methane yield and methane content in a microbial electrolysis cell-coupled anaerobic digestion (MEC-AD) system treating waste activated sludge (WAS) fermentation liquor. Results revealed that Fe-NC maintained a meso-macroporous structure with a large specific surface area of 1381 m<sup>2</sup>/g and superior electrochemical properties. Its calculated specific capacitance and electron transfer resistance were 5.7 and 0.4 times of the carbon felt (CF) group. The bacterial and archaeal gene loads of Fe-NC biofilm after multiple acclimation cycles were 5.69E+10 and 1.86E+9 copies/cm<sup>2</sup> and <em>Proteiniphilum</em> and <em>Methanobacterium</em> were the most enriched syntrophs from stage Ⅰ to stage Ⅱ acclimation. Corresponding maximum methane yield and content achieved were 0.31 m<sup>3</sup> CH<sub>4</sub>/kg COD and 92.8%, and its CO<sub>2</sub>-dependent methane production was improved by 107.6%. Mechanistic investigations showed that Fe-NC biofilm improved enzyme-associated CO<sub>2</sub> reduction pathway companying by promoting the intra- and extracellular electron transfer as well as ATP synthesis, therefore favoring methanogenic energetic metabolism. More importantly, an enhanced proton-coupled electron transfer (PCET) process was proposed within Fe-NC biofilm, providing a synergistic advantage over unbalanced conventional sole electron/proton transfer. This work provides an effective strategy to strengthen the waste-to-energy and biogas upgrading technology, potentially bringing economic benefits to wastewater treatment.\",\"PeriodicalId\":443,\"journal\":{\"name\":\"Water Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":11.4000,\"publicationDate\":\"2024-10-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water Research\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.watres.2024.122675\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.watres.2024.122675","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Enhanced Biofilm-dependent Biogas Upgrading from Waste Activated Sludge Fermentation Liquor in Microbial Electrolysis Cells
This study demonstrated that metal-organic frameworks (MOFs)-derived Fe-NC cathode improved both methane yield and methane content in a microbial electrolysis cell-coupled anaerobic digestion (MEC-AD) system treating waste activated sludge (WAS) fermentation liquor. Results revealed that Fe-NC maintained a meso-macroporous structure with a large specific surface area of 1381 m2/g and superior electrochemical properties. Its calculated specific capacitance and electron transfer resistance were 5.7 and 0.4 times of the carbon felt (CF) group. The bacterial and archaeal gene loads of Fe-NC biofilm after multiple acclimation cycles were 5.69E+10 and 1.86E+9 copies/cm2 and Proteiniphilum and Methanobacterium were the most enriched syntrophs from stage Ⅰ to stage Ⅱ acclimation. Corresponding maximum methane yield and content achieved were 0.31 m3 CH4/kg COD and 92.8%, and its CO2-dependent methane production was improved by 107.6%. Mechanistic investigations showed that Fe-NC biofilm improved enzyme-associated CO2 reduction pathway companying by promoting the intra- and extracellular electron transfer as well as ATP synthesis, therefore favoring methanogenic energetic metabolism. More importantly, an enhanced proton-coupled electron transfer (PCET) process was proposed within Fe-NC biofilm, providing a synergistic advantage over unbalanced conventional sole electron/proton transfer. This work provides an effective strategy to strengthen the waste-to-energy and biogas upgrading technology, potentially bringing economic benefits to wastewater treatment.
期刊介绍:
Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include:
•Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management;
•Urban hydrology including sewer systems, stormwater management, and green infrastructure;
•Drinking water treatment and distribution;
•Potable and non-potable water reuse;
•Sanitation, public health, and risk assessment;
•Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions;
•Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment;
•Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution;
•Environmental restoration, linked to surface water, groundwater and groundwater remediation;
•Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts;
•Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle;
•Socio-economic, policy, and regulations studies.