{"title":"通过调节 m6A 清除剂的 RNA 结合亲和力，实现高度序列特异性、时间可控的 m6A 去甲基化","authors":"Kenko Otonari , Yuri Asami , Kosuke Ogata , Yasushi Ishihama , Shiroh Futaki , Miki Imanishi","doi":"10.1039/d4cc04070h","DOIUrl":null,"url":null,"abstract":"<div><div>Recent advancements in tools using programmable RNA binding proteins and m<sup>6</sup>A-erasers enable sequence-selective and timing-controllable m<sup>6</sup>A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m<sup>6</sup>A-erasers. The modulated m<sup>6</sup>A-erasers achieved sequence-specific and timing-controllable m<sup>6</sup>A demethylation with minimal off-target activity.</div></div>","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":"61 1","pages":"Pages 69-72"},"PeriodicalIF":4.3000,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Highly sequence-specific, timing-controllable m6A demethylation by modulating RNA-binding affinity of m6A erasers†\",\"authors\":\"Kenko Otonari , Yuri Asami , Kosuke Ogata , Yasushi Ishihama , Shiroh Futaki , Miki Imanishi\",\"doi\":\"10.1039/d4cc04070h\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Recent advancements in tools using programmable RNA binding proteins and m<sup>6</sup>A-erasers enable sequence-selective and timing-controllable m<sup>6</sup>A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m<sup>6</sup>A-erasers. The modulated m<sup>6</sup>A-erasers achieved sequence-specific and timing-controllable m<sup>6</sup>A demethylation with minimal off-target activity.</div></div>\",\"PeriodicalId\":67,\"journal\":{\"name\":\"Chemical Communications\",\"volume\":\"61 1\",\"pages\":\"Pages 69-72\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-12-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Communications\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/org/science/article/pii/S1359734524025345\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Communications","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/org/science/article/pii/S1359734524025345","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/22 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Highly sequence-specific, timing-controllable m6A demethylation by modulating RNA-binding affinity of m6A erasers†
Recent advancements in tools using programmable RNA binding proteins and m6A-erasers enable sequence-selective and timing-controllable m6A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m6A-erasers. The modulated m6A-erasers achieved sequence-specific and timing-controllable m6A demethylation with minimal off-target activity.
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ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.
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