{"title":"树蕨类植物近亲繁殖和变异负荷的基因组特征。","authors":"Huiqin Yi, Jing Wang, Shiyong Dong, Ming Kang","doi":"10.1111/tpj.17064","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Ferns (Pteridophyta), as the second largest group of vascular plants, play important roles in ecosystem functioning. Homosporous ferns exhibit a remarkable range of mating systems, from extreme inbreeding to obligate outcrossing, which may have significant evolutionary and ecological implications. Despite their significance, the impact of genome-wide inbreeding on genetic diversity and mutation load within the fern lineage remain largely unexplored. In this study, we utilized whole-genome sequencing to investigate the genomic signatures of inbreeding and genetic load in three <i>Alsophila</i> tree fern species. Our analysis revealed extremely high inbreeding in <i>A. spinulosa</i>, in contrast to the predominantly outcrossing observed in <i>A. costularis</i> and <i>A. latebrosa</i>. This difference likely reflects divergent mating systems and demographic histories. Consistent with its extreme inbreeding propensity, <i>A. spinulosa</i> exhibits reduced genetic diversity and a pronounced decline in effective population size. Comparison of genetic load revealed an overall reduction in deleterious mutations in the highly inbred <i>A. spinulosa</i>, highlighting that long-term inbreeding may have contributed to the purging of strongly deleterious mutations, thereby prolonging the survival of <i>A. spinulosa</i>. Despite this, however, <i>A. spinulosa</i> carries a substantive realized genetic load that may potentially instigate future fitness decline. Our findings illuminate the complex evolutionary interplay between inbreeding and mutation load in homosporous ferns, yielding insights with important implications for the conservation and management of these species.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 4","pages":"1522-1535"},"PeriodicalIF":6.2000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic signatures of inbreeding and mutation load in tree ferns\",\"authors\":\"Huiqin Yi, Jing Wang, Shiyong Dong, Ming Kang\",\"doi\":\"10.1111/tpj.17064\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Ferns (Pteridophyta), as the second largest group of vascular plants, play important roles in ecosystem functioning. Homosporous ferns exhibit a remarkable range of mating systems, from extreme inbreeding to obligate outcrossing, which may have significant evolutionary and ecological implications. Despite their significance, the impact of genome-wide inbreeding on genetic diversity and mutation load within the fern lineage remain largely unexplored. In this study, we utilized whole-genome sequencing to investigate the genomic signatures of inbreeding and genetic load in three <i>Alsophila</i> tree fern species. Our analysis revealed extremely high inbreeding in <i>A. spinulosa</i>, in contrast to the predominantly outcrossing observed in <i>A. costularis</i> and <i>A. latebrosa</i>. This difference likely reflects divergent mating systems and demographic histories. Consistent with its extreme inbreeding propensity, <i>A. spinulosa</i> exhibits reduced genetic diversity and a pronounced decline in effective population size. Comparison of genetic load revealed an overall reduction in deleterious mutations in the highly inbred <i>A. spinulosa</i>, highlighting that long-term inbreeding may have contributed to the purging of strongly deleterious mutations, thereby prolonging the survival of <i>A. spinulosa</i>. Despite this, however, <i>A. spinulosa</i> carries a substantive realized genetic load that may potentially instigate future fitness decline. Our findings illuminate the complex evolutionary interplay between inbreeding and mutation load in homosporous ferns, yielding insights with important implications for the conservation and management of these species.</p>\\n </div>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\"120 4\",\"pages\":\"1522-1535\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17064\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17064","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
蕨类植物(翼手目)是第二大维管束植物类群,在生态系统功能中发挥着重要作用。同孢蕨类植物的交配系统种类繁多,从极端近交到强制外交,这可能会对进化和生态产生重大影响。尽管其意义重大,但全基因组近交对蕨类植物系遗传多样性和变异负荷的影响在很大程度上仍未得到探讨。在这项研究中,我们利用全基因组测序技术研究了三种蕨类植物近交和遗传负荷的基因组特征。我们的分析发现,A. spinulosa 的近交率极高,而在 A. costularis 和 A. latebrosa 中观察到的则主要是外交。这种差异可能反映了不同的交配系统和人口历史。与极度近交倾向相一致的是,A. spinulosa 的遗传多样性减少,有效种群数量明显下降。遗传负荷的比较显示,在高度近亲繁殖的桫椤中,有害突变总体上有所减少,这表明长期近亲繁殖可能有助于清除强有害突变,从而延长了桫椤的生存期。然而,尽管如此,桫椤仍携带着大量已实现的遗传负荷,有可能导致未来的适应性下降。我们的发现揭示了同孢蕨类植物近亲繁殖与突变负荷之间复杂的进化相互作用,对这些物种的保护和管理具有重要意义。
Genomic signatures of inbreeding and mutation load in tree ferns
Ferns (Pteridophyta), as the second largest group of vascular plants, play important roles in ecosystem functioning. Homosporous ferns exhibit a remarkable range of mating systems, from extreme inbreeding to obligate outcrossing, which may have significant evolutionary and ecological implications. Despite their significance, the impact of genome-wide inbreeding on genetic diversity and mutation load within the fern lineage remain largely unexplored. In this study, we utilized whole-genome sequencing to investigate the genomic signatures of inbreeding and genetic load in three Alsophila tree fern species. Our analysis revealed extremely high inbreeding in A. spinulosa, in contrast to the predominantly outcrossing observed in A. costularis and A. latebrosa. This difference likely reflects divergent mating systems and demographic histories. Consistent with its extreme inbreeding propensity, A. spinulosa exhibits reduced genetic diversity and a pronounced decline in effective population size. Comparison of genetic load revealed an overall reduction in deleterious mutations in the highly inbred A. spinulosa, highlighting that long-term inbreeding may have contributed to the purging of strongly deleterious mutations, thereby prolonging the survival of A. spinulosa. Despite this, however, A. spinulosa carries a substantive realized genetic load that may potentially instigate future fitness decline. Our findings illuminate the complex evolutionary interplay between inbreeding and mutation load in homosporous ferns, yielding insights with important implications for the conservation and management of these species.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.