{"title":"基于银纳米颗粒的牙周韧带成纤维细胞的成骨代谢分化对正畸牙齿移动的影响","authors":"Juan Fu, Kun Meng, Qingmin Yuan","doi":"10.1007/s12013-024-01580-7","DOIUrl":null,"url":null,"abstract":"<p><p>It was to clarify the effects of silver nanoparticles (AgNPs) on biological functions of human periodontal ligament fibroblasts (hPDLFs).</p><p><strong>Methods: </strong>AgNPs were synthesized using a tannic acid reduction method and characterized accordingly. Fifteen Sprague-Dawley rats were randomly assigned to Normal group, Group A (orthodontic tooth movement after alveolar bone defect repair with a blood clot), and Group B (orthodontic tooth movement after alveolar bone defect repair with AgNPs), with five rats in each group. Morphological changes in periodontal tissues were visualized. hPDLFs were treated with 0 μM (Ctrl), 25 μM (L-AgNPs), 50 μM (M-AgNPs), and 100 μM (H-AgNPs) AgNPs to assess cell proliferation via the MTT assay, calcification via alizarin red staining, and osteogenic differentiation and genes/proteins' expression associated with the I3K/Akt signaling pathway through quantitative polymerase chain reaction and Western blot.</p><p><strong>Results: </strong>AgNP diameter was approximately 20 nm. Relative to the normal group, both Group A and Group B exhibited increased widths of the periodontal ligament (PDL) while displaying a decrease in cell counts within the PDL (P < 0.05). Furthermore, the L-AgNPs, M-AgNPs, and H-AgNPs groups exhibited a notable elevation in the number of calcified nodules in hPDLFs, along with elevated alkaline phosphatase, Runx2, osteocalcin, osterix, type I collagen, phosphorylated phosphoinositide 3-kinase, and phosphorylated protein kinase B versus Ctrl (P < 0.05).</p><p><strong>Conclusion: </strong>AgNPs are beneficial in enhancing the biological functions of the PDL, promoting the repair and regeneration of periodontal tissues, indicating their potential clinical value in orthodontic treatments.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Osteogenic Metabolic Differentiation of Silver Nanoparticles-based Periodontal Ligament Fibroblasts on Orthodontic Tooth Movement.\",\"authors\":\"Juan Fu, Kun Meng, Qingmin Yuan\",\"doi\":\"10.1007/s12013-024-01580-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>It was to clarify the effects of silver nanoparticles (AgNPs) on biological functions of human periodontal ligament fibroblasts (hPDLFs).</p><p><strong>Methods: </strong>AgNPs were synthesized using a tannic acid reduction method and characterized accordingly. Fifteen Sprague-Dawley rats were randomly assigned to Normal group, Group A (orthodontic tooth movement after alveolar bone defect repair with a blood clot), and Group B (orthodontic tooth movement after alveolar bone defect repair with AgNPs), with five rats in each group. Morphological changes in periodontal tissues were visualized. hPDLFs were treated with 0 μM (Ctrl), 25 μM (L-AgNPs), 50 μM (M-AgNPs), and 100 μM (H-AgNPs) AgNPs to assess cell proliferation via the MTT assay, calcification via alizarin red staining, and osteogenic differentiation and genes/proteins' expression associated with the I3K/Akt signaling pathway through quantitative polymerase chain reaction and Western blot.</p><p><strong>Results: </strong>AgNP diameter was approximately 20 nm. Relative to the normal group, both Group A and Group B exhibited increased widths of the periodontal ligament (PDL) while displaying a decrease in cell counts within the PDL (P < 0.05). Furthermore, the L-AgNPs, M-AgNPs, and H-AgNPs groups exhibited a notable elevation in the number of calcified nodules in hPDLFs, along with elevated alkaline phosphatase, Runx2, osteocalcin, osterix, type I collagen, phosphorylated phosphoinositide 3-kinase, and phosphorylated protein kinase B versus Ctrl (P < 0.05).</p><p><strong>Conclusion: </strong>AgNPs are beneficial in enhancing the biological functions of the PDL, promoting the repair and regeneration of periodontal tissues, indicating their potential clinical value in orthodontic treatments.</p>\",\"PeriodicalId\":510,\"journal\":{\"name\":\"Cell Biochemistry and Biophysics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell Biochemistry and Biophysics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12013-024-01580-7\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Biochemistry and Biophysics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12013-024-01580-7","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的是阐明银纳米粒子（AgNPs）对人类牙周韧带成纤维细胞（hPDLFs）生物功能的影响：方法：采用鞣酸还原法合成 AgNPs，并对其进行表征。将 15 只 Sprague-Dawley 大鼠随机分为正常组、A 组（用血凝块修复牙槽骨缺损后正畸牙齿移动）和 B 组（用 AgNPs 修复牙槽骨缺损后正畸牙齿移动），每组 5 只。观察牙周组织的形态变化。用 0 μM（Ctrl）、25 μM（L-AgNPs）、50 μM（M-AgNPs）和 100 μM（H-AgNPs）AgNPs 处理 hPDLFs，通过 MTT 试验评估细胞增殖情况，通过茜素红染色评估钙化情况，通过定量聚合酶链反应和 Western 印迹评估成骨分化情况以及与 I3K/Akt 信号通路相关的基因/蛋白质表达情况：AgNP直径约为20 nm。与正常组相比，A 组和 B 组的牙周韧带（PDL）宽度均有所增加，而 PDL 内的细胞数量则有所减少（P 结论：A 组和 B 组的牙周韧带宽度均有所增加，而 PDL 内的细胞数量则有所减少：AgNPs 有利于增强牙周韧带的生物功能，促进牙周组织的修复和再生，显示了其在正畸治疗中的潜在临床价值。
Effect of Osteogenic Metabolic Differentiation of Silver Nanoparticles-based Periodontal Ligament Fibroblasts on Orthodontic Tooth Movement.
It was to clarify the effects of silver nanoparticles (AgNPs) on biological functions of human periodontal ligament fibroblasts (hPDLFs).
Methods: AgNPs were synthesized using a tannic acid reduction method and characterized accordingly. Fifteen Sprague-Dawley rats were randomly assigned to Normal group, Group A (orthodontic tooth movement after alveolar bone defect repair with a blood clot), and Group B (orthodontic tooth movement after alveolar bone defect repair with AgNPs), with five rats in each group. Morphological changes in periodontal tissues were visualized. hPDLFs were treated with 0 μM (Ctrl), 25 μM (L-AgNPs), 50 μM (M-AgNPs), and 100 μM (H-AgNPs) AgNPs to assess cell proliferation via the MTT assay, calcification via alizarin red staining, and osteogenic differentiation and genes/proteins' expression associated with the I3K/Akt signaling pathway through quantitative polymerase chain reaction and Western blot.
Results: AgNP diameter was approximately 20 nm. Relative to the normal group, both Group A and Group B exhibited increased widths of the periodontal ligament (PDL) while displaying a decrease in cell counts within the PDL (P < 0.05). Furthermore, the L-AgNPs, M-AgNPs, and H-AgNPs groups exhibited a notable elevation in the number of calcified nodules in hPDLFs, along with elevated alkaline phosphatase, Runx2, osteocalcin, osterix, type I collagen, phosphorylated phosphoinositide 3-kinase, and phosphorylated protein kinase B versus Ctrl (P < 0.05).
Conclusion: AgNPs are beneficial in enhancing the biological functions of the PDL, promoting the repair and regeneration of periodontal tissues, indicating their potential clinical value in orthodontic treatments.
期刊介绍：
Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems
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