根据孕前母体营养状况和分娩方式确定新生儿肠道微生物群的特征。

IF 2.1 3区 医学 Q2 OBSTETRICS & GYNECOLOGY Archives of Gynecology and Obstetrics Pub Date : 2024-10-10 DOI:10.1007/s00404-024-07772-z
Gabriele Luiza Caprara, Otávio von Ameln Lovison, Andreza Francisco Martins, Juliana Rombaldi Bernardi, Marcelo Zubaran Goldani
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引用次数: 0

摘要

目的:本研究旨在根据母体孕前的营养状况和分娩方式确定新生儿肠道微生物群的组成特征:方法:由于当时无法采集胎粪样本,因此在产后 24 至 48 小时之间从新生儿肛门粘膜采集生物样本。并发放了一份一般数据收集问卷。通过对 16S 基因的 v3-v4 超变区进行新一代测序,对样本的微生物组进行了分析。使用观察丰富度和香农多样性指数指标进行α多样性分析,使用加权Unifrac非度量多维尺度进行β多样性分析,使用负二项式Wald检验和最大似然估计广义线性模型系数进行丰度差异分析:结果:与体重指数(BMI)适中的母亲的新生儿相比,肥胖母亲的新生儿表现出较低的α多样性。我们观察到新生儿粪便样本中微生物群落的组成存在差异,其中既有来自超重/肥胖母亲的样本,也有来自孕前体重指数适当母亲的样本。我们观察到分娩方式与新生儿微生物群之间存在明显的相关性。我们发现,无论分娩方式如何,新生儿粪便中微生物群落的整体组成都存在差异:我们的研究结果表明,剖腹产新生儿的微生物群与阴道分娩新生儿的微生物群存在差异,超重/肥胖母亲的新生儿与孕前体重指数适当的母亲的新生儿也存在差异。
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Characterization of newborn gut microbiota according to the pre-gestational maternal nutritional status and delivery mode.

Purpose: The aim of this study is to characterize the composition of the newborn gut microbiota based on the maternal pre-pregnancy nutritional status and the delivery mode.

Methods: A biological sample was collected from the anal mucosa of the newborns between 24 and 48 h after delivery, as it was not possible to collect a meconium sample at that time. A general data collection questionnaire was administered. The microbiome of the samples was analyzed by next-generation sequencing of the hypervariable regions v3-v4 of the 16S gene. Alpha diversity analyses were performed using the Observed Richness and Shannon diversity index metrics and Beta diversity analyses were conducted using Nonmetric multidimensional scaling with Weighted Unifrac, Differential abundance analysis was performed using a Negative Binomial Wald Test with maximum likelihood estimation for coefficients of Generalized Linear Models.

Results: Newborns of obese mothers exhibited lower alpha diversity compared to newborns of mothers with adequate BMI (body mass index). We observed variation in the composition of the microbial community in newborn stool samples, both from mothers with overweight/obesity and those with adequate pre-pregnancy BMI. We observed a visible correlation between the mode of delivery and the newborn's microbiota. We found variation in the overall composition of the microbial community in the stools of newborns, regardless of the delivery mode.

Conclusions: The results of our study demonstrate differences in the microbiota of neonates born via cesarean section compared to those born vaginally as well as differences in newborns of mothers with overweight/obesity compared to those with an adequate pre-pregnancy BMI.

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来源期刊
CiteScore
4.70
自引率
15.40%
发文量
493
审稿时长
1 months
期刊介绍: Founded in 1870 as "Archiv für Gynaekologie", Archives of Gynecology and Obstetrics has a long and outstanding tradition. Since 1922 the journal has been the Organ of the Deutsche Gesellschaft für Gynäkologie und Geburtshilfe. "The Archives of Gynecology and Obstetrics" is circulated in over 40 countries world wide and is indexed in "PubMed/Medline" and "Science Citation Index Expanded/Journal Citation Report". The journal publishes invited and submitted reviews; peer-reviewed original articles about clinical topics and basic research as well as news and views and guidelines and position statements from all sub-specialties in gynecology and obstetrics.
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