Zhaoyu Gong, Yaping Shi, Jian Liu, Ramkumar Sabesan, Ruikang K Wang
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引用次数: 0
摘要
光学视网膜造影术(ORG)是通过测量视网膜对光刺激的反应来评估视网膜功能的一种很有前途的非侵入性客观技术。光学相干断层扫描(OCT)因其三维成像能力、对光刺激引起的纳米级变化的高灵敏度和临床可用性,已成为实施视网膜视网膜造影术的理想工具。尽管 ORG 在实验室环境中已被证明是可行的,但研究级 OCT 系统缺乏令人满意的可用性和成本效益,因此在临床上并不可行。标准的临床光栅扫描 OCT 系统成像速度有限,无法满足测量快速 ORG 反应的要求。为了弥补这一差距,我们在光栅扫描 OCT 系统的基础上引入了闪烁 ORG 模式,该模式与标准临床 OCT 相似。该系统通过创新的两阶段扫描协议和 600 kHz 扫频光源克服了速度限制,实现了有限区域内的重复体积成像和精确视网膜活动测量。此外,光适应 ORG 策略无需暗适应,允许在光照条件下进行检查,从而提高了患者的依从性。我们通过测量五名健康受试者闪烁诱导的感光器反应,测试了这种新的 ORG 方法。结果表明,ORG 反应具有很高的可重复性,并且与闪烁频率和视网膜偏心率有关。这些发现,加上该系统的实用性、成本效益以及与现有技术集成的便捷性,凸显了其在临床应用方面的巨大潜力。
Light-adapted flicker-optoretinography based on raster-scan optical coherence tomography towards clinical translation.
Optoretinography (ORG) is a promising non-invasive and objective technique for assessing retinal function by measuring its response to light stimulation. Optical coherence tomography (OCT) has emerged as a promising tool for implementing ORG due to its three-dimensional imaging capabilities, high sensitivity to nanometer-scale changes induced by light stimulation, and clinical availability. Although ORG has proven feasible in laboratory settings, research-grade OCT systems lack satisfactory usability and cost-effectiveness to be clinically viable. Standard clinical raster-scan OCT systems, with their limited imaging speed, fall short of the requirements for measuring rapid ORG responses. To bridge this gap, we introduce a flicker-ORG modality based on a raster-scan OCT system that resembles standard clinical OCT. This system overcomes speed limitations through an innovative two-stage scanning protocol coupled with a 600 kHz swept source, enabling repeated volume imaging and precise retinal activity measurements over a finite area. Additionally, the light-adapted ORG strategy eliminates the need for dark adaptation, allowing examinations under photopic conditions and thus improving patient compliance. We tested this new ORG method by measuring flicker-induced photoreceptor responses in five healthy subjects. The results demonstrated high repeatability and revealed dependencies of the ORG response on flicker frequency and retinal eccentricity. These findings, combined with the system's utility, cost-effectiveness, and ease of integration into existing technologies, underscore its substantial potential for clinical application.
期刊介绍:
The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including:
Tissue optics and spectroscopy
Novel microscopies
Optical coherence tomography
Diffuse and fluorescence tomography
Photoacoustic and multimodal imaging
Molecular imaging and therapies
Nanophotonic biosensing
Optical biophysics/photobiology
Microfluidic optical devices
Vision research.