抗体库的噬菌体展示筛选：筛选程序。

Cold Spring Harbor protocols Pub Date : 2024-10-15 DOI:10.1101/pdb.prot108602

Haiyong Peng, Christoph Rader

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引用次数: 0

摘要

通过噬菌体展示挖掘天真抗体、免疫抗体和合成抗体库，已被广泛应用于多种物种单克隆抗体的从头生成和体外进化。噬菌体展示抗体库一旦建立，就可以根据所需的抗原结合特性，通过各种不同的策略进行筛选。在这里，我们介绍了用 pComb3 噬菌体家族的噬菌体展示载体生成的抗体库的筛选方法。该方法包括固定抗原、溶液中生物素化抗原和细胞表面抗原的筛选程序。虽然这些抗体库的典型格式是人类 Fab 或嵌合非人/人类 Fab，但本方案中提供的基本选择策略与各种格式的抗体库兼容。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Phage Display Selection of Antibody Libraries: Panning Procedures.

The mining of naive, immune, and synthetic antibody repertoires by phage display has been widely applied to the de novo generation and in vitro evolution of monoclonal antibodies from multiple species. Once built, phage display antibody libraries can be selected by a variety of different strategies tailored toward the desired antigen binding properties. Here, we describe the selection of antibody libraries generated in a phage display vector of the pComb3 phagemid family. The approach includes panning procedures for immobilized antigens, biotinylated antigens in solution, and cell surface antigens. Although the typical format of these antibody libraries is human Fab or chimeric nonhuman/human Fab, the basic selection strategies provided in this protocol are compatible with a variety of formats.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.

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