用于病原体检测的 CRISPR/Cas 和 Argonaute 驱动的横向流动分析。

IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Critical reviews in food science and nutrition Pub Date : 2024-10-21 DOI:10.1080/10408398.2024.2416473
Yaru Li, Lu Zhao, Long Ma, Yunfeng Bai, Feng Feng
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引用次数: 0

摘要

病原体污染是一个紧迫的全球性公共问题,尤其是最近爆发的食源性疾病,引起了全世界的极大关注。CRISPR/Cas 和 Argonaute 等可编程核酸酶具有可编程性和精确的目标序列特异性,有望成为核酸检测工具,并已用于病原体检测的开发。目前,荧光作为主要的信号输出方式,为传感分析提供了一种简单的响应模式。然而,荧光输出对大型仪器的依赖性和输出数据的正确分析限制了其在偏远地区的应用。侧流条带(LFS)作为一种新兴的柔性基质,具有使用方便、快速、可视化、成本低、便携等诸多优点。CRISPR/Cas 和 Argonaute 与 LFS(侧向流动检测)的结合,为病原体检测提供了一种全新的现场检测模式。在综述中,我们首先介绍了 CRISPR/Cas 和 Argonaute 这两种可编程核酸酶,然后介绍了 LFA 的结构、原理和优势。然后,介绍并总结了基于 CRISPR/Cas 和 Argonaute 的病毒、细菌、寄生虫和真菌的多样化工程检测模式。最后,讨论了现场诊断检测所面临的挑战和前景。
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CRISPR/Cas and Argonaute-powered lateral flow assay for pathogens detection.

Pathogens contamination is a pressing global public issue that has garnered significant attention worldwide, especially in light of recent outbreaks of foodborne illnesses. Programmable nucleases like CRISPR/Cas and Argonaute hold promise as tools for nucleic acid testing owning to programmability and the precise target sequence specificity, which has been utilized for the development pathogens detection. At present, fluorescence, as the main signal output method, provides a simple response mode for sensing analysis. However, the dependence of fluorescence output on large instruments and correct analysis of output data limited its use in remote areas. Lateral flow strips (LFS), emerging as a novel flexible substrate, offer a plethora of advantages, encompassing easy-to-use, rapidity, visualization, low-cost, portability, etc. The integration of CRISPR/Cas and Argonaute with LFS, lateral flow assay (LFA), rendered a new and on-site mode for pathogens detection. In the review, we introduced two programmable nucleases CRISPR/Cas and Argonaute, followed by the structure, principle and advantages of LFA. Then diversified engineering detection pattens for viruses, bacteria, parasites, and fungi based on CRISPR/Cas and Argonaute were introduced and summarized. Finally, the challenge and perspectives involved in on-site diagnostic assays were discussed.

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来源期刊
CiteScore
22.60
自引率
4.90%
发文量
600
审稿时长
7.5 months
期刊介绍: Critical Reviews in Food Science and Nutrition serves as an authoritative outlet for critical perspectives on contemporary technology, food science, and human nutrition. With a specific focus on issues of national significance, particularly for food scientists, nutritionists, and health professionals, the journal delves into nutrition, functional foods, food safety, and food science and technology. Research areas span diverse topics such as diet and disease, antioxidants, allergenicity, microbiological concerns, flavor chemistry, nutrient roles and bioavailability, pesticides, toxic chemicals and regulation, risk assessment, food safety, and emerging food products, ingredients, and technologies.
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