颞下颌关节慢性炎症性疼痛大鼠丘脑的 CircRNA 表达谱分析

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-20 DOI:10.1016/j.gene.2024.149024
Haixia Deng , Pan Zhou , Jing Wang , Jie Zeng , Cong Yu
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引用次数: 0

摘要

由颞下颌关节紊乱症(TMDs)引起的口面部疼痛(OFP)十分普遍,约占总人口的 4.6%。颞下颌关节骨关节炎(TMJOA)是TMD的一种特殊类型,是一种常见的退行性疾病,严重影响患者的生活质量。丘脑是口面部疼痛传导通路的中继站,丘脑中差异表达的环状 RNA(DEcircRNA)可能在 TMJOA 的炎症和炎症性疼痛进展中起着至关重要的作用,并可作为潜在的靶标。本研究旨在探讨 circRNA 在颞下颌关节疼痛丘脑中的表达谱。我们通过高通量测序(HT-seq)获得了颞下颌关节疼痛模型大鼠丘脑中的 circRNA 表达谱,并使用反转录实时聚合酶链反应(RT-qPCR)进一步验证了它们的表达,随后对表达数据进行了生物信息学分析。RNA-Seq共鉴定出425个circRNA(DESeq2 p值为0.0)具有显著差异表达,其中188个上调,237个下调。通过 RT-qPCR 验证后,我们利用 miRanda 软件预测了 miRNA 与所鉴定的 circRNAs 的结合位点,以进一步探索 DEcircRNAs 的功能。基因本体(GO)和京都基因组百科全书(KEGG)分析表明,DEcircRNAs主要富集在与突触发育、蛋白质信号转导和修饰、"昼夜节律娱乐"、"MAPK信号通路 "和 "谷氨酸能突触 "相关的通路和功能中。这些研究结果表明,丘脑中的 DEcircRNAs 在颞下颌关节缺失症的进展过程中起着重要作用,可作为基因治疗的候选分子靶点。
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CircRNA expression profiling of the rat thalamus in temporomandibular joint chronic inflammatory pain
Orofacial pain (OFP) induced by temporomandibular disorders (TMDs) is prevalent, affecting approximately 4.6 % of the population. One specific type of TMD is temporomandibular osteoarthritis (TMJOA), a common degenerative disease that significantly impacts patients’ quality of life. Differentially expressed circular RNAs (DEcircRNAs) in the thalamus, which serves as a relay station in the orofacial pain transmission pathway, may play a crucial role and serve as potential target markers for inflammation and the progression of inflammatory pain in TMJOA. The aim of this study was to investigate the expression profile of circRNAs in the thalamus of TMJOA. We obtained the circRNA expression profile from the thalamus of a rat model of TMJOA through high-throughput sequencing (HT-seq) and further validated their expression using reverse transcription real-time polymerase chain reaction (RT-qPCR), followed by bioinformatics analysis of the expression data. A total of 425 circRNAs (DESeq2 p- value < 0.05, |log2FoldChange| > 0.0) were identified as significantly differentially expressed by RNA-Seq, comprising 188 up-regulated and 237 down-regulated circRNAs. After validation via RT-qPCR, we employed miRanda software to predict the binding sites of miRNAs for the identified circRNAs to further explore the functions of DEcircRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that DEcircRNAs were primarily enriched in pathways and functions related to synapse development, protein signaling and modification, ’Circadian entertainment’, the ’MAPK signaling pathway’, and ’Glutamatergic synapse’. These findings suggest that DEcircRNAs in the thalamus play a significant role in the progression of TMJOA and may serve as promising candidate molecular targets for gene therapy.
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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