狗脑脊液中葡萄糖和蛋白质的尿液浸渍棒测定精度。

IF 1.6 2区 农林科学 Q2 VETERINARY SCIENCES Javma-journal of The American Veterinary Medical Association Pub Date : 2024-10-16 DOI:10.2460/javma.24.05.0345
Aryanne R Ottoboni, Luana S Ribeiro, Gladsthon D Sousa Filho, Paulo A O Gonçalves, David H Matta, Adilson D Damasceno, Danieli B Martins, Aude Castel, Bruno B J Torres
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引用次数: 0

摘要

目的与标准方法相比,评估用尿液检测棒(UD)评估犬脑脊液样本中蛋白质和葡萄糖浓度的精确度:方法:使用 UD 和生物化学方法(分别为焦棓酚红法和葡萄糖氧化酶反应法)测量 22 份患有影响中枢神经系统的神经系统疾病的犬脑脊液样本中的蛋白质和葡萄糖。结果被转换成分数,以便对不同方法进行比较。计算方法之间的差异比例及其 CI。针对 CSF 蛋白(15 和 30 mg/dL)和葡萄糖(40 和 100 mg/dL)的两个临界值,确定了 UD 的灵敏度(Se)、特异度(Sp)、阳性预测值(PPV)、阴性预测值(NPV)和准确度(Ac):对于 CSF 蛋白质(22 份错误样本中的 14 份),不同方法之间的差异比例为 64%(95% CI,44% 至 84%),其中 92.9%(14 份样本中的 13 份)的 UD 得分低于生化方法。对于 CSF 葡萄糖,73%(22 份错误样本中的 16 份;95% CI,54% 至 91%)的方法之间存在差异,其中 87.5%(16 份样本中的 14 份)的 UD 得分高于生化方法。当蛋白质(Se,78.9%;Sp,66.7%;PPV,93.7%;NPV,33.3%;Ac,77.3%)和葡萄糖(Se,89.5%;Sp,33.3%;PPV,89.5%;NPV,33.3%;Ac,81.8%)浓度的临界值为 15 毫克/分升时,尿液量尺的结果更好:结论:尿液检测器在估计犬脑脊液蛋白质和葡萄糖浓度方面并不可靠:临床意义:尿液分析仪低估了犬脑脊液中的蛋白质水平,高估了葡萄糖水平,这可能会对诊断产生重大影响,因此不应将其用作床旁检测。
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Accuracy of urinary dipstick for glucose and protein determination in canine cerebrospinal fluid.

Objective: To evaluate the precision of urinary dipstick (UD) to assess protein and glucose concentrations in canine CSF samples compared to the standard methods.

Methods: Cerebrospinal fluid protein and glucose were measured in 22 samples from dogs with neurological diseases affecting the CNS using UD and biochemistry (pyrogallol red and glucose oxidase reaction, respectively). Results were converted into scores to allow comparison between methods. The proportion of divergence between methods and its CI were calculated. The sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), and accuracy (Ac) of UD were determined for 2 cutoff levels of CSF protein (15 and 30 mg/dL) and glucose (40 and 100 mg/dL).

Results: The proportion of divergence between methods was 64% (95% CI, 44% to 84%) for CSF protein (representing 14 of 22 erroneous samples), of which 92.9% (13 of 14) had a UD score lower than biochemistry. For CSF glucose, 73% (16 of 22 erroneous samples; 95% CI, 54% to 91%) had divergence between methods, of which 87.5% (14 of 16) had a UD score higher than biochemistry. Urinary dipstick had better results when the cutoff level was 15 mg/dL for protein (Se, 78.9%; Sp, 66.7%; PPV, 93.7%; NPV, 33.3%; Ac, 77.3%) and 40 mg/dL for glucose (Se, 89.5%; Sp, 33.3%; PPV, 89.5%; NPV, 33.3%; Ac, 81.8%) concentrations.

Conclusions: Urinary dipstick was unreliable in estimating canine CSF protein and glucose concentrations.

Clinical relevance: The UD underestimated protein and overestimated glucose levels in the canine CSF, which could have a significant diagnostic impact and should discourage its use as a bedside test.

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来源期刊
CiteScore
1.60
自引率
15.80%
发文量
539
审稿时长
6-16 weeks
期刊介绍: Published twice monthly, this peer-reviewed, general scientific journal provides reports of clinical research, feature articles and regular columns of interest to veterinarians in private and public practice. The News and Classified Ad sections are posted online 10 days to two weeks before they are delivered in print.
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Electrointestinography, ultrasonographic contractility, and borborygmi of the cecum and colon are not altered by a single episode of hand walking exercise in healthy horses. Successful complete vaginectomy in dorsal recumbency: description of surgical technique without repositioning combining celiotomy and episiotomy in three dogs. Authors speak …. Chicago clinic puts students' knowledge into practice. Collaborate, Educate, Advocate: Join us at the 2025 Veterinary Leadership Conference.
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