Sporosarcina hypophthalmichthys sp.

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-10-13 DOI:10.1002/jobm.202400226
Meghali Bharti, Monika Sharma, Stanzin Choksket, Himani Khurana, Sneha Siwach, Sonakshi Modeel, Suresh Korpole, Ram Krishan Negi
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引用次数: 0

摘要

从印度喜马偕尔邦 Gobindsagar 水库中的鲢鱼(Hypophthalmichthys molitrix)肠道中分离出了一株杆状、运动、革兰氏染色阳性细菌 RKN2T。该菌株与 Sporosarcina koreensis F73T(98.51%)、Sporosarcina luteola Y1T(98.4%)和 Sporosarcina aquimarina SW28T(98.36%)的序列相似度最高,16S rRNA 基因系统进化证实 RKN2T 菌株属于 Sporosarcina 属。RKN2T 与 Sporosarcina koreensis F73T、Sporosarcina luteola Y1T 和 Sporosarcina aquimarina SW28T 的数字 DNA-DNA 杂交值分别为 21.7%、20.6% 和 19.2%,平均核苷酸同一性值分别为 76.42%、80.16% 和 76.51%。菌株 RKN2T 的基因组分析表明其具有多种生物特性,包括硝酸盐还原性、产生碳水化合物活性酶的基因、抗菌化合物以及潜在的芳香化合物和重金属代谢。RKN2T 基因组的 G+C 组成为 52.7%。该菌株可在 10°C 至 40°C(最适温度为 28°C-30°C)、NaCl 浓度高达 6.0%(w/v)和 6.0-8.0 (最适温度为 6.5-7.5)的 pH 值范围内生长。MK-7是主要的呼吸醌,细胞壁肽聚糖为A-4型,主要脂肪酸为前-C15:0、异-C15:0和前-C17:0,主要氨基酸为Lys-Glu。二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺是 RKN2T 菌株的三种主要极性脂质。根据多相学方法,该菌株是孢子瓢虫属(Sporosarcina)的一个新物种,并将 RKN2T 菌株命名为 Sporosarcina hypophthalmichthys sp.RKN2T 为模式菌株(= MCC 4365T = JCM34522T = CCM9112T)。
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Sporosarcina hypophthalmichthys sp. nov. Isolated From Gastrointestinal Tract of Fish Hypophthalmichthys molitrix (Valenciennes, 1844).

A rod-shaped, motile, Gram-stain-positive bacterial strain RKN2T, was isolated from gut of silver carp (Hypophthalmichthys molitrix) residing in Gobindsagar reservoir, Himachal Pradesh, India. Having the greatest sequence similarity to Sporosarcina koreensis F73T (98.51%), Sporosarcina luteola Y1T (98.4%) and Sporosarcina aquimarina SW28T (98.36%), the 16S rRNA gene phylogeny confirmed the belonging of strain RKN2T to genus Sporosarcina. Digital DNA-DNA hybridization values were 21.7%, 20.6%, and 19.2%, and average nucleotide identity values were 76.42%, 80.16%, 76.51%, of strain RKN2T with Sporosarcina koreensis F73T, Sporosarcina luteola Y1T, and Sporosarcina aquimarina SW28T, respectively. The genomic analysis of strain RKN2T showed various biological properties including nitrate reduction, genes responsible for carbohydrate-active enzymes production, antimicrobial compounds, as well as potential metabolism of aromatic compounds and heavy metals. G+C composition of RKN2T genome was 52.7%. This strain can grow in temperatures between 10°C and 40°C (optimum, 28°C-30°C), NaCl concentrations up to 6.0% (w/v), and 6.0-8.0 (optimum, 6.5-7.5) pH range. MK-7 was the dominant respiratory quinone, A-4 type cell wall peptidoglycan was present with anteiso-C15:0, iso-C15: 0, and anteiso-C17:0 being the major fatty acids and Lys-Glu being main amino acids. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were the strain RKN2T's three main polar lipids. The strain is a novel species under genus Sporosarcina based on polyphasic approach and the name Sporosarcina hypophthalmichthys sp. nov. is given for strain RKN2T. RKN2T is a type strain (= MCC 4365T = JCM34522T = CCM9112T).

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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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