用于高选择性和高灵敏度检测真实水样中 Hg2+ 的反应性荧光和比色探针。

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-10-19 DOI:10.1007/s10895-024-03992-3
Jiarui Yang, Kaiqiang Zhang, Yong Zhao, Yanxi Song, Yihan Wu, Hongqi Li
{"title":"用于高选择性和高灵敏度检测真实水样中 Hg2+ 的反应性荧光和比色探针。","authors":"Jiarui Yang, Kaiqiang Zhang, Yong Zhao, Yanxi Song, Yihan Wu, Hongqi Li","doi":"10.1007/s10895-024-03992-3","DOIUrl":null,"url":null,"abstract":"<p><p>Construction of efficient chemosensors for highly specific and sensitive detection of mercury ions remains a great challenge. In this work a highly selective and sensitive probe CY was designed and synthesized by using coumarin fluorophore as the matrix and thioacetal moiety as the reactive recognition site for Hg<sup>2+</sup>. By virtue of the thiophilicity of Hg<sup>2+</sup>, probe CL could be hydrolyzed to deprotect and the thioacetal was transformed to the acyl group after the addition of Hg<sup>2+</sup>, the blue-green fluorescence was quenched and meanwhile the solution changed from light green to yellow. The detection limit of probe CY for Hg<sup>2+</sup> was as low as 6.8 nM, and it could completely react with Hg<sup>2+</sup> within 3 min. Moreover, probe CY exhibited good resistance against interference from competitive metal ions and biothiols, high stability in pH 1-11 and applicability for fluorogenic and chromogenic dual-modal detection of Hg<sup>2+</sup> in real water samples over a broad range of pH 5-10.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Reactive Fluorescent and Colorimetric Probe for Highly Selective and Sensitive Detection of Hg<sup>2+</sup> in Real Water Samples.\",\"authors\":\"Jiarui Yang, Kaiqiang Zhang, Yong Zhao, Yanxi Song, Yihan Wu, Hongqi Li\",\"doi\":\"10.1007/s10895-024-03992-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Construction of efficient chemosensors for highly specific and sensitive detection of mercury ions remains a great challenge. In this work a highly selective and sensitive probe CY was designed and synthesized by using coumarin fluorophore as the matrix and thioacetal moiety as the reactive recognition site for Hg<sup>2+</sup>. By virtue of the thiophilicity of Hg<sup>2+</sup>, probe CL could be hydrolyzed to deprotect and the thioacetal was transformed to the acyl group after the addition of Hg<sup>2+</sup>, the blue-green fluorescence was quenched and meanwhile the solution changed from light green to yellow. The detection limit of probe CY for Hg<sup>2+</sup> was as low as 6.8 nM, and it could completely react with Hg<sup>2+</sup> within 3 min. Moreover, probe CY exhibited good resistance against interference from competitive metal ions and biothiols, high stability in pH 1-11 and applicability for fluorogenic and chromogenic dual-modal detection of Hg<sup>2+</sup> in real water samples over a broad range of pH 5-10.</p>\",\"PeriodicalId\":15800,\"journal\":{\"name\":\"Journal of Fluorescence\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-10-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fluorescence\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s10895-024-03992-3\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-024-03992-3","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

摘要

构建高效的化学传感器来高度特异和灵敏地检测汞离子仍然是一项巨大的挑战。本研究以香豆素荧光团为基质,以硫代乙缩醛为 Hg2+ 的反应识别位点,设计并合成了一种高选择性、高灵敏度的探针 CY。由于 Hg2+ 的亲硫性,探针 CL 在加入 Hg2+ 后可水解脱保护,硫代缩醛转化为酰基,蓝绿色荧光被淬灭,同时溶液由浅绿色变为黄色。探针 CY 对 Hg2+ 的检测限低至 6.8 nM,并能在 3 分钟内与 Hg2+ 完全反应。此外,探针 CY 对竞争性金属离子和生物硫醇具有良好的抗干扰性,在 pH 值为 1-11 的条件下具有很高的稳定性,可在 pH 值为 5-10 的大范围内用于真实水样中 Hg2+ 的荧光和显色双模式检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Reactive Fluorescent and Colorimetric Probe for Highly Selective and Sensitive Detection of Hg2+ in Real Water Samples.

Construction of efficient chemosensors for highly specific and sensitive detection of mercury ions remains a great challenge. In this work a highly selective and sensitive probe CY was designed and synthesized by using coumarin fluorophore as the matrix and thioacetal moiety as the reactive recognition site for Hg2+. By virtue of the thiophilicity of Hg2+, probe CL could be hydrolyzed to deprotect and the thioacetal was transformed to the acyl group after the addition of Hg2+, the blue-green fluorescence was quenched and meanwhile the solution changed from light green to yellow. The detection limit of probe CY for Hg2+ was as low as 6.8 nM, and it could completely react with Hg2+ within 3 min. Moreover, probe CY exhibited good resistance against interference from competitive metal ions and biothiols, high stability in pH 1-11 and applicability for fluorogenic and chromogenic dual-modal detection of Hg2+ in real water samples over a broad range of pH 5-10.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
期刊最新文献
Correction: Influence of Viscosity and Temperature on Rotational Dynamics of 3-(1H-benzo[d]Imidazol-2-yl)-7-(Diethyl Amino)-2H-chromen-2-one (Polar Laser dye) in Highly Viscous Solvent: Low and High Viscous Range. Feasibility of Simultaneous Artificial Intelligence-Assisted and NIR Fluorescence Navigation for Anatomical Recognition in Laparoscopic Colorectal Surgery. Multifunctional Copper Nanoclusters for the Detection of pH and Berberine Hydrochloride. Preliminary Development of DNA Aptamer Quantum Dot-Based Competitive Lateral Flow Assays for Saxitoxin and Tetrodotoxin. Highly Potent Fluorenone Azine-based ESIPT Active Fluorophores for Cellular Viscosity Detection and Bioimaging Applications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1