感染Ageratum conyzoides的中国木瓜卷叶病毒的分子鉴定和遗传多样性分析

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2024-10-01 DOI:10.5423/PPJ.NT.04.2024.0066
Liping Zhang, Shujie Wu, Meisheng Zhao, Hussein Ghanem, Gentu Wu, Mingjun Li, Ling Qing
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引用次数: 0

摘要

木瓜卷叶病毒(PaLCuCNV)是一种危害性植物病原体,会给作物造成重大损失。研究人员从Ageratum conyzoides中获得了3个PaLCuCNV分离株的完整基因组,并将其与GenBank中的68个参考分离株结合起来,利用专门的计算工具进行了全面的遗传多样性分析。序列比对结果显示,71 个 PaLCuCNV 分离物的核苷酸序列相似度从 85.3% 到 99.9% 不等。通过系统进化分析,71个PaLCuCNV序列被聚类为5个组,没有观察到基因分化与宿主物种或地理起源之间有明显的相关性。此外,在所有 PaLCuCNV 分离物中发现了 13 个重组事件。遗传多样性分析表明,PaLCuCNV 群体在不断扩大和进化,这得到了中性模型的支持。此外,在不同的病毒种群之间观察到了明显的遗传分化,这主要归因于遗传漂变。总之,我们的研究结果为 PaLCuCNV 的检测、遗传变异和进化动态提供了有价值的见解。
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Molecular Identification and Genetic Diversity Analysis of Papaya Leaf Curl China Virus Infecting Ageratum conyzoides.

Papaya leaf curl China virus (PaLCuCNV) is a damaging plant pathogen causing substantial losses to crop. The complete genomes of three PaLCuCNV isolates from Ageratum conyzoides were obtained and combined with the 68 reference isolates in GenBank for comprehensive genetic diversity analyses using specialized computational tools. Sequence alignment revealed nucleotide sequence similarity ranging from 85.3% to 99.9% among 71 PaLCuCNV isolates. Employing phylogenetic analysis, 71 PaLCuCNV sequences were clustered into five groups, with no significant correlation observed between genetic differentiation and either host species or geographical origin. Additionally, 13 recombination events across all PaLCuCNV isolates were identified. Genetic diversity analysis indicated the ongoing expansion and evolution of PaLCuCNV populations, supported by a neutral model. Moreover, significant genetic differentiation was observed among distinct viral populations, primarily attributed to genetic drift. Overall, our findings provide valuable insights into the detection, genetic variation, and evolutionary dynamics of PaLCuCNV.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
期刊最新文献
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