角膜炎患者和非角膜炎患者泪液中的凋亡、基质降解和抗氧化生物标志物水平:一项横断面研究。

IF 4.1 3区 医学 Q1 OPHTHALMOLOGY Contact Lens & Anterior Eye Pub Date : 2024-10-17 DOI:10.1016/j.clae.2024.102315
Anna Maria Roszkowska, Umberto Camellin, Francesco Franchina, Danilo Alunni-Fegatelli, Giuseppe Lombardo, Sebastiano Serrao, Rita Mencucci, Annarita Vestri, Marco Lombardo
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引用次数: 0

摘要

目的:评估泪液中一组凋亡、基质降解和抗氧化生物标志物的水平,包括金属蛋白酶9(MMP9)、高流动性组框1(HMGB1)和细胞外超氧化物歧化酶3(SOD3):使用夹心-ELISA 商用试剂盒检测合格参与者泪液中三种泪液生物标志物的表达。进行线性逻辑回归分析,以确定泪液生物标志物是否与临床表现的角膜炎相关。采用 10 倍交叉验证进行 ROC 曲线分析,以评估模型的预测准确性:本研究共招募了 81 名年龄在 30-48 岁之间的参与者,其中 48 人为角膜炎患者,33 人为年龄匹配的健康受试者。三种泪液生物标志物水平的线性组合(AUC = 0.811;CI 95 %:0.712-0.911)准确地显示了角膜炎的存在;较高水平的 MMP9(奇数比:1.069;CI 95 %:1.029-1.130)和HMGB1(OR:1.011;CI 95 %:1.003-1.022)以及较低水平的SOD3(OR:0.994;CI 95 %:0.989-0.997)与较高的角膜炎概率显著相关:对一组泪液中的 MMP9、HMGB1 和 SOD3 生物标志物水平进行多变量分析,证实了角膜炎患者眼表的慢性炎症状态。
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Tear levels of apoptotic, matrix-degrading and antioxidant biomarkers in patients with and without keratoconus: A cross sectional study.

Purpose: To assess the tear levels of a set of apoptotic, matrix-degrading and antioxidant biomarkers, including Metalloproteinase 9 (MMP9), High Mobility Group Box 1 (HMGB1) and Superoxide Dismutase 3-Extracellular (SOD3).

Methods: Sandwich-ELISA commercial kits were used to test the expression of the three tear biomarkers in the lacrimal fluid of eligible participants. Linear logistic regression analysis was performed todetermine whether the set of tear biomarkers could be associated with clinically manifest keratoconus. ROC curve analysis using 10-fold cross-validation was performedto evaluate the prediction accuracy of the model.

Results: Eighty-one participants aged 30-48 years old were enrolled in this study; 48 were patients with keratoconus and 33 were age-matched healthy subjects. The linear combination of the three tear biomarkers levels (AUC = 0.811; CI 95 %: 0.712-0.911) accurately indicated the existence of keratoconus; higher levels of MMP9 (Odd Ratio: 1.069; CI 95 %: 1.029-1.130) and HMGB1 (OR: 1.011; CI 95 %: 1.003-1.022) and lower levels of SOD3 (OR: 0.994; CI 95 %: 0.989-0.997) were significantly associated with a higher probability of keratoconus.

Conclusion: Multivariable analysis of the set of tear levels of MMP9, HMGB1 and SOD3 biomarkers confirmed a chronic state of inflammation in the ocular surface of patients with keratoconus.

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来源期刊
CiteScore
7.60
自引率
18.80%
发文量
198
审稿时长
55 days
期刊介绍: Contact Lens & Anterior Eye is a research-based journal covering all aspects of contact lens theory and practice, including original articles on invention and innovations, as well as the regular features of: Case Reports; Literary Reviews; Editorials; Instrumentation and Techniques and Dates of Professional Meetings.
期刊最新文献
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