Mírian Ívens Fagundes , Nathalia Coral Galvani , Ellen De-Pieri , Igor Ramos Lima , Maria Eduarda Anastácio Borges Corrêa , Lidiane Anastácio Cruz , Thiago Antônio Moretti de Andrade , Carlos Chávez-Olórtegui , Paulo Cesar Lock Silveira , Ricardo Andrez Machado-de-Ávila
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The animals underwent necrotic induction with 250 µg <em>B. jararaca</em> venom (BjV) and were treated with ultrasound (U) at 1 MHz frequency at an intensity of 0.8 W/cm² for 5 min, 30 mg/L GNPs, and anti-bothropic serum (AS) in the following combinations: saline solution (SS); BjV; BjV + AS; BjV + AS + U; BjV + GNPs + AS; BjV + GNPs + AS + U; BjV + GNPs; BjV + GNPs + U; and BjV + U. The necrotic area, histology, oxidative stress, oxidative damage, and anti-oxidant system were assessed to evaluate the effects of the treatments.</div></div><div><h3>Results</h3><div>Treatments that included GNPs, U, and/or AS demonstrated reductions in necrotic area, increases in angiogenesis and fibroblast means, decreases in inflammatory infiltrates, and improvements in collagen synthesis. Additionally, there was an increase in oxidants and oxidant damage within the gastrocnemius muscle, along with an increase in anti-oxidants. 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引用次数: 0
摘要
目的方法：将超声波与金纳米粒子（GNPs）结合使用，开发一种可增强由箭毒双蛛毒液引起的病变恢复的治疗方法：方法：将 108 只瑞士小鼠分为 9 组。方法：将 108 只瑞士小鼠分为 9 组，每组用 250 µg B. jararaca 毒液（BjV）诱导坏死，然后用频率为 1 MHz、强度为 0.8 W/cm² 的超声波（U）治疗 5 分钟。对坏死面积、组织学、氧化应激、氧化损伤和抗氧化系统进行评估，以评价处理的效果：结果：包括 GNPs、U 和/或 AS 在内的治疗可减少坏死面积，增加血管生成和成纤维细胞数量，减少炎症浸润，改善胶原合成。此外，腓肠肌内氧化剂和氧化剂损伤增加，抗氧化剂增加。此外，似乎还产生了全身效应，改善了心血管和肾脏的抗氧化系统：结论：使用 GNPs 和 U 可有效治疗由 B. jararaca 蛇毒引起的病变。
Ultrasound and Gold Nanoparticles Improve Tissue Repair for Muscle Injury Caused by Snake Venom
Objective
To develop a treatment that enhances recovery from envenomation-induced lesions caused by Bothrops jararaca venom by using ultrasound in combination with gold nanoparticles (GNPs).
Methods
A total of 108 Swiss mice were arranged into nine groups. The animals underwent necrotic induction with 250 µg B. jararaca venom (BjV) and were treated with ultrasound (U) at 1 MHz frequency at an intensity of 0.8 W/cm² for 5 min, 30 mg/L GNPs, and anti-bothropic serum (AS) in the following combinations: saline solution (SS); BjV; BjV + AS; BjV + AS + U; BjV + GNPs + AS; BjV + GNPs + AS + U; BjV + GNPs; BjV + GNPs + U; and BjV + U. The necrotic area, histology, oxidative stress, oxidative damage, and anti-oxidant system were assessed to evaluate the effects of the treatments.
Results
Treatments that included GNPs, U, and/or AS demonstrated reductions in necrotic area, increases in angiogenesis and fibroblast means, decreases in inflammatory infiltrates, and improvements in collagen synthesis. Additionally, there was an increase in oxidants and oxidant damage within the gastrocnemius muscle, along with an increase in anti-oxidants. Furthermore, systemic effects appear to have been achieved, improving the anti-oxidant system at the cardiovascular and renal levels.
Conclusion
The use of GNPs and U may be effective at treating lesions caused by B. jararaca snake venom.
期刊介绍：
Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.
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