表皮复合寄生虫的基因组编辑:现状、挑战和未来的可能性》。

IF 3.7 4区 生物学 Q2 GENETICS & HEREDITY CRISPR Journal Pub Date : 2024-10-10 DOI:10.1089/crispr.2024.0032
Ethel Webi, Hussein M Abkallo, George Obiero, Paul Ndegwa, Shengsong Xie, Shuhong Zhao, Vishvanath Nene, Lucilla Steinaa
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引用次数: 0

摘要

成簇的有规则间隔短回文重复序列(CRISPR)-CRISPR相关蛋白(Cas)技术已经彻底改变了各种生物系统的基因组编辑,其中包括类囊虫门。本综述介绍了 CRISPR-Cas9 编辑技术的现状、挑战以及在疟原虫、弓形虫、Theileria、巴贝丝菌和隐孢子虫等类群寄生虫中的应用。文章讨论了在这些寄生虫中成功实施的基于CRISPR-Cas9的技术,重点介绍了从精确基因修饰到全基因组筛选所取得的阶段性成果。此外,综述还讨论了阻碍高效基因组编辑的挑战,包括寄生虫复杂的生命周期、多细胞内阶段以及缺乏强大的遗传工具。它还进一步探讨了基因组编辑的伦理和政策考量,以及 CRISPR-Cas 在类凋亡寄生虫中应用的未来前景。
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Genome Editing in Apicomplexan Parasites: Current Status, Challenges, and Future Possibilities.

Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) technology has revolutionized genome editing across various biological systems, including the Apicomplexa phylum. This review describes the status, challenges, and applications of CRISPR-Cas9 editing technology in apicomplexan parasites, such as Plasmodium, Toxoplasma, Theileria, Babesia, and Cryptosporidium. The discussion encompasses successfully implemented CRISPR-Cas9-based techniques in these parasites, highlighting the achieved milestones, from precise gene modifications to genome-wide screening. In addition, the review addresses the challenges hampering efficient genome editing, including the parasites' complex life cycles, multiple intracellular stages, and the lack of robust genetic tools. It further explores the ethical and policy considerations surrounding genome editing and the future perspectives of CRISPR-Cas applications in apicomplexan parasites.

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来源期刊
CRISPR Journal
CRISPR Journal Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.30
自引率
2.70%
发文量
76
期刊介绍: In recognition of this extraordinary scientific and technological era, Mary Ann Liebert, Inc., publishers recently announced the creation of The CRISPR Journal -- an international, multidisciplinary peer-reviewed journal publishing outstanding research on the myriad applications and underlying technology of CRISPR. Debuting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a high-profile venue for groundbreaking research, as well as lively and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the Mary Ann Liebert, Inc. portfolio, which includes GEN (Genetic Engineering & Biotechnology News) and more than 80 leading peer-reviewed journals.
期刊最新文献
Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods. CRISPR-GRIT: Guide RNAs with Integrated Repair Templates Enable Precise Multiplexed Genome Editing in the Diploid Fungal Pathogen Candida albicans. Genome Editing in Apicomplexan Parasites: Current Status, Challenges, and Future Possibilities. Mutation-Specific CRISPR Targeting with SaCas9 and AsCas12a Restores Therapeutic Sensitivity in Treatment-Resistant Melanoma. Characterization of Research Support of Genome Editing Technologies and Transition to Clinical Trials.
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