外多糖不利于具有截短脂多糖核心的裂殖单胞菌 HH103 突变体的共生性能。

IF 4.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Journal Pub Date : 2024-10-25 DOI:10.1042/bcj20240599
Francisco Fuentes-Romero,Marcello Mercogliano,Stefania De Chiara,Cynthia Alías-Villegas,Pilar Navarro-Gómez,Sebastián Acosta-Jurado,Alba Silipo,Carlos Medina,Miguel-Ángel Rodríguez-Carvajal,Marta S Dardanelli,José-Enrique Ruiz-Sainz,Francisco-Javier López-Baena,Antonio Molinaro,José-María Vinardell,Flaviana Di Lorenzo
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引用次数: 0

摘要

固氮根瘤菌与豆科植物的共生依赖于双方在整个相互作用过程中分子信号的复杂交换。在细菌方面,不同的表面多糖,如脂多糖(LPS)和外多糖(EPS),可能对相互作用的成功起着重要作用。在之前的工作中,我们研究了两个受 rkpK 和 lpsL 基因影响的 HH103 突变体,这两个基因分别负责产生葡萄糖醛酸和半乳糖醛酸。这两个突变体产生的 LPS 都发生了改变,此外,rkpK 突变体还缺乏 EPS。这些突变体在与 Glycine max 和 Vigna unguiculata 共生时受到的影响不同,lpsL 突变体比 rkpK 突变体受到的影响更大。在本研究中,我们进一步研究了 HH103 lpsL 和 rkpK 突变体的 LPS 结构和共生能力。结果表明,这两种菌株产生的 LPS 结构相同,都有一个不含尿酸的截短核心寡糖。我们发现,lpsL突变体与大肠杆菌(Macroptilium atropurpureum)和甘草(Glycyrrhiza uralensis)的共生性能比rkpK突变体差。在 HH103 lpsL 中引入 exoA 突变(可避免产生 EPS)后,它与 G. max、M. atropurpureum 和 G. uralensis 的共生性能提高到了 HH103 rkpK 的水平,这表明 EPS 的存在可能掩盖了前一突变体产生的截短 LPS。
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Exopolysaccharide is detrimental for the symbiotic performance of Sinorhizobium fredii HH103 mutants with a truncated lipopolysaccharide core.
The nitrogen-fixing rhizobia-legume symbiosis relies on a complex interchange of molecular signals between the two partners during the whole interaction. On the bacterial side, different surface polysaccharides, such as lipopolysaccharide (LPS) and exopolysaccharide (EPS), might play important roles for the success of the interaction. In a previous work we studied two Sinorhizobium fredii HH103 mutants affected in the rkpK and lpsL genes, which are responsible for the production of glucuronic acid and galacturonic acid, respectively. Both mutants produced an altered LPS, and the rkpK mutant, in addition, lacked EPS. These mutants were differently affected in symbiosis with Glycine max and Vigna unguiculata, with the lpsL mutant showing a stronger impairment than the rkpK mutant. In the present work we have further investigated the LPS structure and the symbiotic abilities of the HH103 lpsL and rkpK mutants. We demonstrate that both strains produce the same LPS, with a truncated core oligosaccharide devoid of uronic acids. We show that the symbiotic performance of the lpsL mutant with Macroptilium atropurpureum and Glycyrrhiza uralensis is worse than that of the rkpK mutant. Introduction of an exoA mutation (which avoids EPS production) in HH103 lpsL improved its symbiotic performance with G. max, M. atropurpureum, and G. uralensis to the level exhibited by HH103 rkpK, suggesting that the presence of EPS might hide the truncated LPS produced by the former mutant.
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来源期刊
Biochemical Journal
Biochemical Journal 生物-生化与分子生物学
CiteScore
8.00
自引率
0.00%
发文量
255
审稿时长
1 months
期刊介绍: Exploring the molecular mechanisms that underpin key biological processes, the Biochemical Journal is a leading bioscience journal publishing high-impact scientific research papers and reviews on the latest advances and new mechanistic concepts in the fields of biochemistry, cellular biosciences and molecular biology. The Journal and its Editorial Board are committed to publishing work that provides a significant advance to current understanding or mechanistic insights; studies that go beyond observational work using in vitro and/or in vivo approaches are welcomed. Painless publishing: All papers undergo a rigorous peer review process; however, the Editorial Board is committed to ensuring that, if revisions are recommended, extra experiments not necessary to the paper will not be asked for. Areas covered in the journal include: Cell biology Chemical biology Energy processes Gene expression and regulation Mechanisms of disease Metabolism Molecular structure and function Plant biology Signalling
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