Katja Stojkovič, Camilla Canovi, Kim-Cuong Le, Iftikhar Ahmad, Ioana Gaboreanu, Sofie Johansson, Nicolas Delhomme, Ulrika Egertsdotter, Nathaniel R Street
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This study presents a comprehensive data resource profiling gene expression during zygotic and somatic embryo development to support studies aiming to advance understanding of gene regulatory programmes controlling embryo development. Transcriptome expression patterns were analysed during zygotic embryogenesis (ZE) in Norway spruce, including separated samples of the female gametophytes and Zem, and at multiple stages during SE. Expression data from eight developmental stages of SE, starting with pro-embryogenic masses (PEMs) up until germination, revealed extensive modulation of the transcriptome between the early and mid-stage maturing embryos and at the transition of desiccated embryos to germination. Comparative analysis of gene expression changes during ZE and SE identified differences in the pattern of gene expression changes and functional enrichment of these provided insight into the associated biological processes. Orthologs of transcription factors known to regulate embryo development in angiosperms were differentially regulated during Zem and Sem development and in the different zygotic embryo tissues, providing clues to the differences in development observed between Zem and Sem. 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引用次数: 0
摘要
体细胞胚胎发生(SE)是研究胚胎发育的强大模型系统,也是扩大挪威云杉(Picea abies L. Karst)精英和气候适应性遗传材料可用性的重要方法。然而,在体细胞胚胎(Sem)的发育过程中,有几个步骤与子代胚胎(Zem)的发育相比并不理想。人们对这些差异知之甚少,因此在植物生产过程中造成了巨大的产量损失,限制了 SE 植物经济高效的大规模生产。本研究提供了一个全面的数据资源,分析了合子胚和体细胞胚发育过程中的基因表达,以支持旨在促进对控制胚发育的基因调控程序的了解的研究。研究人员分析了挪威云杉子代胚胎发生(ZE)过程中的转录组表达模式,包括雌配子体和Zem的分离样本,以及SE过程中多个阶段的转录组表达模式。从原胚胎块(PEM)开始直至萌芽的八个发育阶段的表达数据显示,转录组在早期和中期成熟胚胎之间以及在干燥胚胎向萌芽的过渡阶段发生了广泛的变化。对ZE和SE期间基因表达变化的比较分析发现了基因表达变化模式的差异,对这些差异的功能富集有助于深入了解相关的生物过程。已知调控被子植物胚胎发育的转录因子的同源物在Zem和Sem发育过程中以及在不同的合子胚胎组织中受到不同的调控,从而为观察到Zem和Sem发育过程中的差异提供了线索。该资源是目前可用于探索针叶树胚胎发育的最全面的数据集。
A transcriptome atlas of zygotic and somatic embryogenesis in Norway spruce.
Somatic embryogenesis (SE) is a powerful model system for studying embryo development and an important method for scaling up availability of elite and climate-adapted genetic material of Norway spruce (Picea abies L. Karst). However, there are several steps during the development of the somatic embryo (Sem) that are suboptimal compared to zygotic embryo (Zem) development. These differences are poorly understood and result in substantial yield losses during plant production, which limits cost-effective large-scale production of SE plants. This study presents a comprehensive data resource profiling gene expression during zygotic and somatic embryo development to support studies aiming to advance understanding of gene regulatory programmes controlling embryo development. Transcriptome expression patterns were analysed during zygotic embryogenesis (ZE) in Norway spruce, including separated samples of the female gametophytes and Zem, and at multiple stages during SE. Expression data from eight developmental stages of SE, starting with pro-embryogenic masses (PEMs) up until germination, revealed extensive modulation of the transcriptome between the early and mid-stage maturing embryos and at the transition of desiccated embryos to germination. Comparative analysis of gene expression changes during ZE and SE identified differences in the pattern of gene expression changes and functional enrichment of these provided insight into the associated biological processes. Orthologs of transcription factors known to regulate embryo development in angiosperms were differentially regulated during Zem and Sem development and in the different zygotic embryo tissues, providing clues to the differences in development observed between Zem and Sem. This resource represents the most comprehensive dataset available for exploring embryo development in conifers.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.