中红外光谱与化学计量学相结合,作为监测热应激和日粮干预对哺乳母猪影响的工具。

IF 3 3区 地球科学 Q2 BIOPHYSICS International Journal of Biometeorology Pub Date : 2024-10-26 DOI:10.1007/s00484-024-02792-5
M Navarro, A Coba, M Muller, E Roura, D Cozzolino
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引用次数: 0

摘要

超高产泌乳母猪的热应激被认为是降低采食量、产奶量和福利的一个因素,会对农场生产率造成重大损失。环境高热时个体的体温调节能力决定了动物在长期和反复事件中的适应能力。本研究旨在评估衰减全反射(ATR)中红外(MIR)光谱作为一种高通量方法,在热应激条件下识别泌乳母猪血浆和牛奶中应激标记物的能力。利用线性判别分析(LDA)和主成分分析对近红外光谱进行了分析,并利用交叉验证进行了验证。结果表明,结合化学计量学,近红外光谱能够识别与热应力相关的光谱变化,其波长分别对应于酰胺基团(蛋白质)(在 1065 和 1635 cm-1 之间的区域观察到最高载荷)、脂类和不饱和脂肪酸(在 1746 和 3063 cm-1 之间的区域)、脂多糖(在 1247 cm-1 之间)和碳水化合物(在 1050 cm-1 附近的区域)。这些结果还表明,这些波长提供的信息可用作母猪适应高热的代谢标记。最后得出的结论是,近红外光谱是一种快速、廉价的工具,能够检测和评估哺乳母猪高热引起的主要生化变化,有助于制定缓解管理策略,如控制饮食。
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Mid infrared spectroscopy combined with chemometrics as tool to monitor the impact of heat stress and dietary interventions in lactating sows.

Heat stress in hyper-prolific lactating sows is recognised as a factor reducing feed intake, milk production, and welfare, with significant losses in farm productivity. Individual capacities for body thermoregulation during environmental hyperthermia determine the adaptation of the animal during long and recurrent events. This study aimed to evaluate the ability of attenuated total reflectance (ATR) mid infrared (MIR) spectroscopy as a high-throughput method to identify markers of stress in plasma and milk collected from lactating sows under heat stress conditions fed with two levels of protein in the diet defined as low (16%) and standard (20%). The MIR spectra were analysed using linear discriminant analysis (LDA) and principal component analysis and validated using cross-validation. The results obtained indicated that MIR spectroscopy, in combination with chemometrics, was able to identify changes in the spectra associated with heat stress in wavenumbers corresponding with amide groups (proteins) (highest loadings observed in the regions between1065 and 1635 cm-1), lipids and unsaturated fatty acids (regions between 1746 and 3063 cm-1), lipo-polysaccharides (in 1247 cm-1) and carbohydrates (around the region1050 cm-1). These results also indicated that the information provided by these wavenumbers can be used as metabolic markers of the adaptation of the sows to hyperthermia. It was concluded that MIR spectroscopy is a rapid and inexpensive tool capable of detecting and evaluating the main biochemical changes of hyperthermia on lactating sows, facilitating the development of palliative management strategies such as dietary manipulations.

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来源期刊
CiteScore
6.40
自引率
9.40%
发文量
183
审稿时长
1 months
期刊介绍: The Journal publishes original research papers, review articles and short communications on studies examining the interactions between living organisms and factors of the natural and artificial atmospheric environment. Living organisms extend from single cell organisms, to plants and animals, including humans. The atmospheric environment includes climate and weather, electromagnetic radiation, and chemical and biological pollutants. The journal embraces basic and applied research and practical aspects such as living conditions, agriculture, forestry, and health. The journal is published for the International Society of Biometeorology, and most membership categories include a subscription to the Journal.
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