My Ahmed El Amiri, Hamid Kabdy, Abdelfatah Aitbaba, Jawad Laadraoui, Rachida Aboufatima, Loubna Elyazouli, Baslam Abdelmounaim, Soad Moubtakir, Stefania Garzoli, Chait Abderrahman
{"title":"牛至水提取物的化学成分、抗氧化能力、急性毒性和抗痛性分析","authors":"My Ahmed El Amiri, Hamid Kabdy, Abdelfatah Aitbaba, Jawad Laadraoui, Rachida Aboufatima, Loubna Elyazouli, Baslam Abdelmounaim, Soad Moubtakir, Stefania Garzoli, Chait Abderrahman","doi":"10.1002/cbdv.202401580","DOIUrl":null,"url":null,"abstract":"<p><p>Phytotherapy, the use of plant-derived compounds for medicinal purposes, is increasingly recognized as a vital component of modern therapeutics. This study investigates the aqueous extract of Origanum majorana L. (AEOM), evaluating its phytochemical composition, safety, antioxidant activity, and pharmacological effects. Analysis via LC-MS/MS identified a variety of phenolic compounds, with gallic acid, caffeic acid, and chlorogenic acid standing out for their pronounced bioactive properties. Quantitative analysis revealed that AEOM contains significant amounts of polyphenols (186.06 ± 0.1 mg GAE/g), flavonoids (72.3 ± 0.9 mg QE/g), and condensed tannins (4.49 ± 0.08 mg CE/g). The extract exhibited strong antioxidant activity, with an IC₅₀ value of 2.23 ± 0.03 mg/mL in the DPPH assay, and demonstrated considerable reducing power (FRAP value of 1.9 ± 0.01 mg Fe²⁺/g). Pharmacological evaluation showed that AEOM significantly reduced pain in an animal model, suggesting potential analgesic properties. Acute toxicity studies indicated no adverse effects on kidney and liver function or blood parameters at doses up to 800 mg/kg. The analgesic effect is likely mediated by flavonoids in the extract, which may inhibit pain pathways. These findings suggest that O. majorana has promising therapeutic applications, particularly as a natural analgesic agent.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of Chemical Composition, Antioxidant Capacity, Acute Toxicity, and Antinociceptive Properties of Aqueous Extract of Origanum majorana L.\",\"authors\":\"My Ahmed El Amiri, Hamid Kabdy, Abdelfatah Aitbaba, Jawad Laadraoui, Rachida Aboufatima, Loubna Elyazouli, Baslam Abdelmounaim, Soad Moubtakir, Stefania Garzoli, Chait Abderrahman\",\"doi\":\"10.1002/cbdv.202401580\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Phytotherapy, the use of plant-derived compounds for medicinal purposes, is increasingly recognized as a vital component of modern therapeutics. This study investigates the aqueous extract of Origanum majorana L. (AEOM), evaluating its phytochemical composition, safety, antioxidant activity, and pharmacological effects. Analysis via LC-MS/MS identified a variety of phenolic compounds, with gallic acid, caffeic acid, and chlorogenic acid standing out for their pronounced bioactive properties. Quantitative analysis revealed that AEOM contains significant amounts of polyphenols (186.06 ± 0.1 mg GAE/g), flavonoids (72.3 ± 0.9 mg QE/g), and condensed tannins (4.49 ± 0.08 mg CE/g). The extract exhibited strong antioxidant activity, with an IC₅₀ value of 2.23 ± 0.03 mg/mL in the DPPH assay, and demonstrated considerable reducing power (FRAP value of 1.9 ± 0.01 mg Fe²⁺/g). Pharmacological evaluation showed that AEOM significantly reduced pain in an animal model, suggesting potential analgesic properties. Acute toxicity studies indicated no adverse effects on kidney and liver function or blood parameters at doses up to 800 mg/kg. The analgesic effect is likely mediated by flavonoids in the extract, which may inhibit pain pathways. These findings suggest that O. majorana has promising therapeutic applications, particularly as a natural analgesic agent.</p>\",\"PeriodicalId\":9878,\"journal\":{\"name\":\"Chemistry & Biodiversity\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-10-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemistry & Biodiversity\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1002/cbdv.202401580\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry & Biodiversity","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1002/cbdv.202401580","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Analysis of Chemical Composition, Antioxidant Capacity, Acute Toxicity, and Antinociceptive Properties of Aqueous Extract of Origanum majorana L.
Phytotherapy, the use of plant-derived compounds for medicinal purposes, is increasingly recognized as a vital component of modern therapeutics. This study investigates the aqueous extract of Origanum majorana L. (AEOM), evaluating its phytochemical composition, safety, antioxidant activity, and pharmacological effects. Analysis via LC-MS/MS identified a variety of phenolic compounds, with gallic acid, caffeic acid, and chlorogenic acid standing out for their pronounced bioactive properties. Quantitative analysis revealed that AEOM contains significant amounts of polyphenols (186.06 ± 0.1 mg GAE/g), flavonoids (72.3 ± 0.9 mg QE/g), and condensed tannins (4.49 ± 0.08 mg CE/g). The extract exhibited strong antioxidant activity, with an IC₅₀ value of 2.23 ± 0.03 mg/mL in the DPPH assay, and demonstrated considerable reducing power (FRAP value of 1.9 ± 0.01 mg Fe²⁺/g). Pharmacological evaluation showed that AEOM significantly reduced pain in an animal model, suggesting potential analgesic properties. Acute toxicity studies indicated no adverse effects on kidney and liver function or blood parameters at doses up to 800 mg/kg. The analgesic effect is likely mediated by flavonoids in the extract, which may inhibit pain pathways. These findings suggest that O. majorana has promising therapeutic applications, particularly as a natural analgesic agent.
期刊介绍:
Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level.
Since 2017, Chemistry & Biodiversity is published in an online-only format.