{"title":"切割植物纤维的锐利解决方案。","authors":"Talia Jacobson , Mair Edwards , Cătălin Voiniciuc","doi":"10.1016/j.copbio.2024.103219","DOIUrl":null,"url":null,"abstract":"<div><div>Plant cells sequester atmospheric carbon in thick walls containing heterogenous networks of cellulose and hemicelluloses (e.g. xylan and mannan), surrounded by additional polymers. Plants and microbes secrete glycosyl hydrolases that act alone or as modular complexes to modify extracellular polysaccharides. While several carbohydrate-active enzymes have been successfully targeted for crop improvement, additional hydrolytic proteins or more efficient versions are needed for the circular bioeconomy. Here, we discuss persistent challenges and emerging opportunities to enhance extracellular enzymes for use in plants or as tools to process biomass for various products. In addition to the design of minimal cellulosomes that combine microbial scaffolding proteins and polysaccharide-degrading enzymes, we review three major strategies that could improve the properties of plant-derived glycosyl hydrolases.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"90 ","pages":"Article 103219"},"PeriodicalIF":7.1000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sharp solutions to cleave plant fibers\",\"authors\":\"Talia Jacobson , Mair Edwards , Cătălin Voiniciuc\",\"doi\":\"10.1016/j.copbio.2024.103219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Plant cells sequester atmospheric carbon in thick walls containing heterogenous networks of cellulose and hemicelluloses (e.g. xylan and mannan), surrounded by additional polymers. Plants and microbes secrete glycosyl hydrolases that act alone or as modular complexes to modify extracellular polysaccharides. While several carbohydrate-active enzymes have been successfully targeted for crop improvement, additional hydrolytic proteins or more efficient versions are needed for the circular bioeconomy. Here, we discuss persistent challenges and emerging opportunities to enhance extracellular enzymes for use in plants or as tools to process biomass for various products. In addition to the design of minimal cellulosomes that combine microbial scaffolding proteins and polysaccharide-degrading enzymes, we review three major strategies that could improve the properties of plant-derived glycosyl hydrolases.</div></div>\",\"PeriodicalId\":10833,\"journal\":{\"name\":\"Current opinion in biotechnology\",\"volume\":\"90 \",\"pages\":\"Article 103219\"},\"PeriodicalIF\":7.1000,\"publicationDate\":\"2024-10-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current opinion in biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0958166924001551\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current opinion in biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0958166924001551","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Plant cells sequester atmospheric carbon in thick walls containing heterogenous networks of cellulose and hemicelluloses (e.g. xylan and mannan), surrounded by additional polymers. Plants and microbes secrete glycosyl hydrolases that act alone or as modular complexes to modify extracellular polysaccharides. While several carbohydrate-active enzymes have been successfully targeted for crop improvement, additional hydrolytic proteins or more efficient versions are needed for the circular bioeconomy. Here, we discuss persistent challenges and emerging opportunities to enhance extracellular enzymes for use in plants or as tools to process biomass for various products. In addition to the design of minimal cellulosomes that combine microbial scaffolding proteins and polysaccharide-degrading enzymes, we review three major strategies that could improve the properties of plant-derived glycosyl hydrolases.
期刊介绍：
Current Opinion in Biotechnology (COBIOT) is renowned for publishing authoritative, comprehensive, and systematic reviews. By offering clear and readable syntheses of current advances in biotechnology, COBIOT assists specialists in staying updated on the latest developments in the field. Expert authors annotate the most noteworthy papers from the vast array of information available today, providing readers with valuable insights and saving them time.
As part of the Current Opinion and Research (CO+RE) suite of journals, COBIOT is accompanied by the open-access primary research journal, Current Research in Biotechnology (CRBIOT). Leveraging the editorial excellence, high impact, and global reach of the Current Opinion legacy, CO+RE journals ensure they are widely read resources integral to scientists' workflows.
COBIOT is organized into themed sections, each reviewed once a year. These themes cover various areas of biotechnology, including analytical biotechnology, plant biotechnology, food biotechnology, energy biotechnology, environmental biotechnology, systems biology, nanobiotechnology, tissue, cell, and pathway engineering, chemical biotechnology, and pharmaceutical biotechnology.
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