Current opinion in biotechnology最新文献

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Proteomics insights into the fungal-mediated bioremediation of environmental contaminants 蛋白质组学对真菌介导的环境污染物生物修复的启示。

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-10-10 DOI: 10.1016/j.copbio.2024.103213

As anthropogenic activities continue to introduce various contaminants into the environment, the need for effective monitoring and bioremediation strategies is critical. Fungi, with their diverse enzymatic arsenal, offer promising solutions for the biotransformation of many pollutants. While conventional research reports on ligninolytic, oxidoreductive, and cytochrome P450 (CYP) enzymes, the vast potential of fungi, with approximately 10 345 protein sequences per species, remains largely untapped. This review describes recent advancements in fungal proteomics instruments as well as software and highlights their detoxification mechanisms and biochemical pathways. Additionally, it highlights lesser-known fungal enzymes with potential applications in environmental biotechnology. By reviewing the benefits and challenges associated with proteomics tools, we hope to summarize and promote the studies of fungi and fungal proteins relevant in the environment.

随着人类活动不断向环境中引入各种污染物，有效的监测和生物修复策略显得至关重要。真菌拥有多种酶库，为许多污染物的生物转化提供了前景广阔的解决方案。传统的研究报告涉及木质素分解酶、氧化还原酶和细胞色素 P450 (CYP) 酶，而真菌的巨大潜力（每个物种约有 10 345 个蛋白质序列）在很大程度上仍未得到开发。本综述介绍了真菌蛋白质组学仪器和软件的最新进展，并重点介绍了真菌的解毒机制和生化途径。此外，它还重点介绍了鲜为人知的、具有环境生物技术应用潜力的真菌酶。通过回顾与蛋白质组学工具相关的益处和挑战，我们希望总结并促进与环境相关的真菌和真菌蛋白质研究。

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Methane to bioproducts: unraveling the potential of methanotrophs for biomanufacturing 从甲烷到生物产品：揭示甲烷营养体在生物制造方面的潜力。

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-10-04 DOI: 10.1016/j.copbio.2024.103210

With the continuous increase in the world population, anthropogenic activities will generate more waste and create greenhouse gases such as methane, amplifying global warming. The biological conversion of methane into biochemicals is a sustainable solution to sequester and convert this greenhouse gas. Methanotrophic bacteria fulfill this role by utilizing methane as a feedstock while manufacturing various bioproducts. Recently, methanotrophs have made their mark in industrial biomanufacturing. However, unlike glucose-utilizing model organisms such as Escherichia coli and Saccharomyces cerevisiae, methanotrophs do not have established transformation methods and genetic tools, making these organisms challenging to engineer. Despite these challenges, recent advancements in methanotroph engineering demonstrate great promise, showcasing these C1-carbon-utilizing microbes as prospective hosts for bioproduction. This review discusses the recent developments and challenges in strain engineering, biomolecule production, and process development methodologies in the methanotroph field.

随着世界人口的不断增加，人类活动将产生更多的废物，并产生甲烷等温室气体，加剧全球变暖。通过生物转化将甲烷转化为生化物质是封存和转化这种温室气体的可持续解决方案。养甲烷细菌利用甲烷作为原料，制造各种生物产品，从而发挥了这一作用。最近，甲烷营养细菌在工业生物制造领域大显身手。然而，与大肠杆菌和酿酒酵母等利用葡萄糖的模式生物不同，甲烷营养菌没有成熟的转化方法和遗传工具，因此这些生物的工程设计具有挑战性。尽管存在这些挑战，但最近在甲烷营养体工程学方面取得的进展展示了巨大的前景，表明这些 C1 碳利用微生物有望成为生物生产的宿主。本综述讨论了甲烷营养体领域在菌种工程、生物大分子生产和工艺开发方法方面的最新进展和挑战。

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Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide 提高生物技术工艺效率的热力学工具：以一氧化碳生产聚（3-羟基丁酸）为例。

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-10-01 DOI: 10.1016/j.copbio.2024.103212

Modern biotechnology requires the integration of several disciplines, with thermodynamics being a crucial one. Experimental approaches frequently used in biotechnology, such as rewiring of metabolic networks or culturing of micro-organisms in engineered environments, can benefit from the application of thermodynamic tools. In this paper, we provide an overview of several thermodynamic tools that are useful for the design and optimization of biotechnological processes, and we demonstrate their potential application in the production of poly-(3-hydroxybutyrate) (PHB) from carbon monoxide (CO). We discuss how these tools can aid in the design of metabolic engineering strategies, the calculation of expected yields, the assessment of the thermodynamic feasibility of the targeted conversions, the identification of potential thermodynamic bottlenecks, and the selection of genetic engineering targets. Although we illustrate these tools using the specific example of PHB production from CO, they can be applied to other substrates and products.

现代生物技术需要整合多个学科，其中热力学是一个重要学科。生物技术中经常使用的实验方法，如重新连接代谢网络或在工程环境中培养微生物，都可以从热力学工具的应用中获益。本文概述了几种有助于设计和优化生物技术过程的热力学工具，并展示了这些工具在利用一氧化碳（CO）生产聚-（3-羟基丁酸）（PHB）过程中的潜在应用。我们讨论了这些工具如何帮助设计代谢工程策略、计算预期产量、评估目标转化的热力学可行性、识别潜在的热力学瓶颈以及选择基因工程目标。虽然我们使用从 CO 生产 PHB 的具体实例来说明这些工具，但它们也可应用于其他底物和产品。

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Editorial overview: Chemical biotechnology paving the way for a sustainable future 编辑综述：化学生物技术为可持续未来铺平道路

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-28 DOI: 10.1016/j.copbio.2024.103215

引用次数: 0

Editorial overview: Plant synthetic biology 编辑综述：植物合成生物学

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-27 DOI: 10.1016/j.copbio.2024.103211

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Food industry side streams: an unexploited source for biotechnological phosphorus upcycling 食品工业副产品流：生物技术磷循环利用的一个未开发来源

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-25 DOI: 10.1016/j.copbio.2024.103209

The phosphorus shortage is an unavoidable challenge that requires strategies to replace phosphorus sourced from ores. Food industry by-products are an unscoped resource for sustainable phosphorus recovery. Recent advances include biotechnological phosphorus upcycling from phytate-rich plant residues to polyphosphate as a food additive. The valorization of by-products such as deoiled seeds or brans additionally provides low-phosphorus feed and thereby minimizes the environmental burden. Phytate reduction in a cereal-rich diet by adding enzyme formulation is a further strategy that limits its antinutritive effect. However, sustainable P-management depends on phytases that have been customized and enhanced for thermostability and specific activity. The circular phosphorus economy is driven by emerging value chains and maturing phosphorus recovery technologies for market entry.

磷短缺是一个不可避免的挑战，需要制定战略来替代从矿石中获取的磷。食品工业的副产品是一种尚未开发的可持续磷回收资源。最近的进展包括利用生物技术从富含植酸的植物残渣中回收磷，将其转化为聚磷酸盐作为食品添加剂。副产品（如脱油种子或麸皮）的增值还可提供低磷饲料，从而最大限度地减轻环境负担。通过添加酶制剂来减少富含植酸的谷物饲料中的植酸，也是一种限制其抗营养作用的策略。然而，可持续的磷管理取决于经过定制和强化的植酸酶的热稳定性和特定活性。新兴的价值链和成熟的磷回收技术将推动磷循环经济进入市场。

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Analysis of polyphosphate in mammalian cells and tissues: methods, functions and challenges 哺乳动物细胞和组织中多磷酸盐的分析：方法、功能和挑战

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-24 DOI: 10.1016/j.copbio.2024.103208

Polyphosphates play a crucial role in various biological processes, such as blood coagulation, energy homeostasis, and cellular stress response. However, their isolation, detection, and quantification present significant challenges. These difficulties arise primarily from their solubility, low concentration in mammals, and structural similarity to other ubiquitous biopolymers. This review provides an overview of the current understanding of polyphosphates in mammals, including their proposed functions and tissue distribution. It also examines key isolation techniques, such as chromatography and precipitation, alongside detection methods, such as colorimetric assays and enzymatic digestion. The strengths and limitations of these methods are discussed, as well as the challenges in preserving polyphosphate integrity. Recent advancements in isolation and detection are also highlighted, offering a comprehensive perspective essential for advancing polyphosphate research.

多磷酸盐在血液凝固、能量平衡和细胞应激反应等各种生物过程中发挥着至关重要的作用。然而，它们的分离、检测和定量却面临着巨大的挑战。这些困难主要来自于它们的可溶性、在哺乳动物体内的低浓度以及与其他无处不在的生物聚合物在结构上的相似性。本综述概述了目前对哺乳动物体内多磷酸盐的了解，包括它们的拟议功能和组织分布。综述还探讨了色谱法和沉淀法等关键分离技术，以及比色法和酶解法等检测方法。报告讨论了这些方法的优势和局限性，以及在保持多磷酸盐完整性方面所面临的挑战。此外，还重点介绍了分离和检测方面的最新进展，为推进多磷酸盐研究提供了一个至关重要的全面视角。

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Polyphosphate accumulation in microalgae and cyanobacteria: recent advances and opportunities for phosphorus upcycling 微藻类和蓝藻中聚磷酸盐的积累：最新进展和磷的循环利用机会

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-19 DOI: 10.1016/j.copbio.2024.103207

Phosphorus (P) must continuously be added to soils as it is lost in the food chain and via leaching. Unfortunately, the mining and import of P to produce fertiliser is unsustainable and costly. Potential solutions to the global issues of P rock depletion and pollution lie in microalgae and cyanobacteria. With an ability to intracellularly store P as polyphosphates, microalgae and cyanobacteria could provide the basis for removing P from water streams, thereby mitigating eutrophication, and even enabling P recovery as P-rich biomass. Metabolic engineering or changes in growing conditions have been demonstrated to improve P removal and recovery by triggering polyphosphates synthesis in the laboratory. This now needs to be replicated at full scale.

磷（P）在食物链和沥滤过程中会流失，因此必须不断向土壤中添加磷。遗憾的是，开采和进口磷来生产化肥是不可持续的，而且成本高昂。微藻和蓝藻是解决全球钾资源枯竭和污染问题的潜在办法。微藻类和蓝藻能够在细胞内以多磷酸盐的形式储存钾，可为去除水流中的钾提供基础，从而减轻富营养化，甚至还能以富含钾的生物质形式回收钾。在实验室中，代谢工程或生长条件的改变已被证明可以通过触发聚磷酸盐的合成来改善对磷的去除和回收。现在需要进行大规模复制。

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The phosphorus challenge: biotechnology approaches for a sustainable phosphorus system 磷挑战：可持续磷系统的生物技术方法

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-18 DOI: 10.1016/j.copbio.2024.103197

Phosphorus (P) is essential for growing crops, but the supply of high-quality phosphate rock reserves used for fertilizer production is finite while losses of P from the food/waste system cause considerable environmental damage. A variety of emerging approaches in biotechnology are reviewed that hold promise for improving the sustainability of P use in the food/water systems. These include improved sensors, cell culture approaches to meat production, bio-based P adsorption and transformation strategies, advancements in understanding of polyphosphate-accumulating organisms, and new approaches involving biomineralization and anaerobic treatment. By advancing these technologies to scale, progress can be made in developing a circular phosphorus economy that improves food security while protecting drinking water and aquatic ecosystems.

磷（P）对作物生长至关重要，但用于化肥生产的优质磷矿石储量有限，而食物/废物系统中磷的损失对环境造成了相当大的破坏。本报告综述了生物技术中的各种新兴方法，这些方法有望改善食物/水系统中磷的可持续利用。这些方法包括改进的传感器、肉类生产的细胞培养方法、基于生物的磷吸附和转化策略、对聚磷酸盐积累生物的进一步了解，以及涉及生物矿化和厌氧处理的新方法。通过推动这些技术的规模化发展，可以在发展循环磷经济方面取得进展，从而在提高粮食安全的同时保护饮用水和水生生态系统。

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The prospect of superior yeast for winemaking: recent successes through bioprospecting 酿酒用优质酵母的前景：最近通过生物勘探取得的成功

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology

Pub Date : 2024-09-16 DOI: 10.1016/j.copbio.2024.103200

This article provides a selective review of recent reports that describe developments in the pursuit of superior yeast for winemaking through bioprospecting. In recent years, the focus of researchers and starter-culture companies has broadened. No longer are the targets merely strains that are reliable and sensorily inoffensive. Rather, efforts have expanded to seek much greater precision in these age-old targets and/or strains that address aspects of sustainability by enabling reduced waste, fewer chemicals or less energy input.

本文有选择性地回顾了最近的一些报道，这些报道描述了通过生物勘探追求酿酒用优质酵母的发展情况。近年来，研究人员和酵母培养公司的关注点不断扩大。他们的目标不再仅仅是那些可靠的、感官上无攻击性的菌株。相反，人们的努力已经扩展到在这些古老的目标中寻求更高精度的菌株和/或通过减少废物、减少化学品或能源投入来解决可持续发展问题的菌株。

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