用于软组织修复的纯化和低温灭菌注射用胶原蛋白的生物相容性研究:大鼠肌肉内植入。

IF 5 3区 化学 Q1 POLYMER SCIENCE Gels Pub Date : 2024-09-26 DOI:10.3390/gels10100619
Tae-Hoon Koo, Jason K Lee, Shawn P Grogan, Ho Jong Ra, Darryl D D'Lima
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引用次数: 0

摘要

以胶原蛋白为基础的生物材料的临床应用正在迅速扩大,尤其是在组织工程和美容方面。虽然口服补充剂和注射皮肤促进剂在增强皮肤健康方面很受欢迎,但支持其有效性的临床证据仍然有限。注射产品显示出活化皮肤的潜力,但由于灭菌方面的挑战和生物污染的风险,安全问题依然存在。传统的消毒方法(加热和辐照)会使胶原蛋白变性。本研究通过引入一种新技术来解决这些问题:胶原蛋白凝胶的双重过滤和低温蒸汽灭菌。体外测试证明了胶原蛋白的无菌性,并证实胶原蛋白在加工和灭菌后未出现细胞毒性、降解、完整性和粘度特性变化。用胶原蛋白凝胶培养人的真皮成纤维细胞时,胶原蛋白凝胶能诱导新胶原蛋白的表达和增殖。一项体内研究发现,13 周内对大鼠没有不良影响,植入部位也没有明显病变。这些结果表明,这种加工胶原凝胶的新方法是一种安全有效的皮肤促进剂。先进的加工方法有可能降低与注射胶原蛋白产品相关的安全风险,但还需要进一步的研究来验证其生物有效性和临床益处。
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Biocompatibility Study of Purified and Low-Temperature-Sterilized Injectable Collagen for Soft Tissue Repair: Intramuscular Implantation in Rats.

The clinical application of collagen-based biomaterials is expanding rapidly, especially in tissue engineering and cosmetics. While oral supplements and injectable skin boosters are popular for enhancing skin health, clinical evidence supporting their effectiveness remains limited. Injectable products show potential in revitalizing skin, but safety concerns persist due to challenges in sterilization and the risk of biological contamination. Traditional methods of sterilization (heat and irradiation) can denature collagen. This study addresses these issues by introducing a novel technique: the double filtration and low-temperature steam sterilization of a collagen gel. In vitro tests documented the sterility and confirmed that the collagen did not show cytotoxicity, degradation, integrity, and viscosity characteristics changes after the processing and sterilization. The collagen gel induced new collagen expression and the proliferation of human dermal fibroblasts when the cells were cultured with the collagen gel. An in vivo study found no adverse effects in rats or significant lesions at the implantation site over 13 weeks. These results suggest that this novel method to process collagen gels is a safe and effective skin booster. Advanced processing methods are likely to mitigate the safety risks associated with injectable collagen products, though further research is needed to validate their biological effectiveness and clinical benefits.

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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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