用于软组织修复的纯化和低温灭菌注射用胶原蛋白的生物相容性研究:大鼠肌肉内植入。

IF 5 3区 化学 Q1 POLYMER SCIENCE Gels Pub Date : 2024-09-26 DOI:10.3390/gels10100619
Tae-Hoon Koo, Jason K Lee, Shawn P Grogan, Ho Jong Ra, Darryl D D'Lima
{"title":"用于软组织修复的纯化和低温灭菌注射用胶原蛋白的生物相容性研究:大鼠肌肉内植入。","authors":"Tae-Hoon Koo, Jason K Lee, Shawn P Grogan, Ho Jong Ra, Darryl D D'Lima","doi":"10.3390/gels10100619","DOIUrl":null,"url":null,"abstract":"<p><p>The clinical application of collagen-based biomaterials is expanding rapidly, especially in tissue engineering and cosmetics. While oral supplements and injectable skin boosters are popular for enhancing skin health, clinical evidence supporting their effectiveness remains limited. Injectable products show potential in revitalizing skin, but safety concerns persist due to challenges in sterilization and the risk of biological contamination. Traditional methods of sterilization (heat and irradiation) can denature collagen. This study addresses these issues by introducing a novel technique: the double filtration and low-temperature steam sterilization of a collagen gel. In vitro tests documented the sterility and confirmed that the collagen did not show cytotoxicity, degradation, integrity, and viscosity characteristics changes after the processing and sterilization. The collagen gel induced new collagen expression and the proliferation of human dermal fibroblasts when the cells were cultured with the collagen gel. An in vivo study found no adverse effects in rats or significant lesions at the implantation site over 13 weeks. These results suggest that this novel method to process collagen gels is a safe and effective skin booster. Advanced processing methods are likely to mitigate the safety risks associated with injectable collagen products, though further research is needed to validate their biological effectiveness and clinical benefits.</p>","PeriodicalId":12506,"journal":{"name":"Gels","volume":"10 10","pages":""},"PeriodicalIF":5.0000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508103/pdf/","citationCount":"0","resultStr":"{\"title\":\"Biocompatibility Study of Purified and Low-Temperature-Sterilized Injectable Collagen for Soft Tissue Repair: Intramuscular Implantation in Rats.\",\"authors\":\"Tae-Hoon Koo, Jason K Lee, Shawn P Grogan, Ho Jong Ra, Darryl D D'Lima\",\"doi\":\"10.3390/gels10100619\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The clinical application of collagen-based biomaterials is expanding rapidly, especially in tissue engineering and cosmetics. While oral supplements and injectable skin boosters are popular for enhancing skin health, clinical evidence supporting their effectiveness remains limited. Injectable products show potential in revitalizing skin, but safety concerns persist due to challenges in sterilization and the risk of biological contamination. Traditional methods of sterilization (heat and irradiation) can denature collagen. This study addresses these issues by introducing a novel technique: the double filtration and low-temperature steam sterilization of a collagen gel. In vitro tests documented the sterility and confirmed that the collagen did not show cytotoxicity, degradation, integrity, and viscosity characteristics changes after the processing and sterilization. The collagen gel induced new collagen expression and the proliferation of human dermal fibroblasts when the cells were cultured with the collagen gel. An in vivo study found no adverse effects in rats or significant lesions at the implantation site over 13 weeks. These results suggest that this novel method to process collagen gels is a safe and effective skin booster. Advanced processing methods are likely to mitigate the safety risks associated with injectable collagen products, though further research is needed to validate their biological effectiveness and clinical benefits.</p>\",\"PeriodicalId\":12506,\"journal\":{\"name\":\"Gels\",\"volume\":\"10 10\",\"pages\":\"\"},\"PeriodicalIF\":5.0000,\"publicationDate\":\"2024-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508103/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gels\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.3390/gels10100619\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gels","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3390/gels10100619","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

以胶原蛋白为基础的生物材料的临床应用正在迅速扩大,尤其是在组织工程和美容方面。虽然口服补充剂和注射皮肤促进剂在增强皮肤健康方面很受欢迎,但支持其有效性的临床证据仍然有限。注射产品显示出活化皮肤的潜力,但由于灭菌方面的挑战和生物污染的风险,安全问题依然存在。传统的消毒方法(加热和辐照)会使胶原蛋白变性。本研究通过引入一种新技术来解决这些问题:胶原蛋白凝胶的双重过滤和低温蒸汽灭菌。体外测试证明了胶原蛋白的无菌性,并证实胶原蛋白在加工和灭菌后未出现细胞毒性、降解、完整性和粘度特性变化。用胶原蛋白凝胶培养人的真皮成纤维细胞时,胶原蛋白凝胶能诱导新胶原蛋白的表达和增殖。一项体内研究发现,13 周内对大鼠没有不良影响,植入部位也没有明显病变。这些结果表明,这种加工胶原凝胶的新方法是一种安全有效的皮肤促进剂。先进的加工方法有可能降低与注射胶原蛋白产品相关的安全风险,但还需要进一步的研究来验证其生物有效性和临床益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Biocompatibility Study of Purified and Low-Temperature-Sterilized Injectable Collagen for Soft Tissue Repair: Intramuscular Implantation in Rats.

The clinical application of collagen-based biomaterials is expanding rapidly, especially in tissue engineering and cosmetics. While oral supplements and injectable skin boosters are popular for enhancing skin health, clinical evidence supporting their effectiveness remains limited. Injectable products show potential in revitalizing skin, but safety concerns persist due to challenges in sterilization and the risk of biological contamination. Traditional methods of sterilization (heat and irradiation) can denature collagen. This study addresses these issues by introducing a novel technique: the double filtration and low-temperature steam sterilization of a collagen gel. In vitro tests documented the sterility and confirmed that the collagen did not show cytotoxicity, degradation, integrity, and viscosity characteristics changes after the processing and sterilization. The collagen gel induced new collagen expression and the proliferation of human dermal fibroblasts when the cells were cultured with the collagen gel. An in vivo study found no adverse effects in rats or significant lesions at the implantation site over 13 weeks. These results suggest that this novel method to process collagen gels is a safe and effective skin booster. Advanced processing methods are likely to mitigate the safety risks associated with injectable collagen products, though further research is needed to validate their biological effectiveness and clinical benefits.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
期刊介绍: The journal Gels (ISSN 2310-2861) is an international, open access journal on physical (supramolecular) and chemical gel-based materials. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the maximum length of the papers, and full experimental details must be provided so that the results can be reproduced. Short communications, full research papers and review papers are accepted formats for the preparation of the manuscripts. Gels aims to serve as a reference journal with a focus on gel materials for researchers working in both academia and industry. Therefore, papers demonstrating practical applications of these materials are particularly welcome. Occasionally, invited contributions (i.e., original research and review articles) on emerging issues and high-tech applications of gels are published as special issues.
期刊最新文献
Dual-Action Gemcitabine Delivery: Chitosan-Magnetite-Zeolite Capsules for Targeted Cancer Therapy and Antibacterial Defense. Emulsion Structural Remodeling in Milk and Its Gelling Products: A Review. Process Mapping of the Sol-Gel Transition in Acid-Initiated Sodium Silicate Solutions. Microencapsulation Efficiency of Carboxymethylcellulose, Gelatin, Maltodextrin, and Acacia for Aroma Preservation in Jasmine Instant Tea. Cross-Linked Polyimide Aerogels with Excellent Thermal and Mechanical Properties.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1