间充质干细胞和人脐静脉内皮细胞在添加了羟基磷灰石的三维多孔水凝胶中的流动条件下的骨球发育。

IF 5 3区 化学 Q1 POLYMER SCIENCE Gels Pub Date : 2024-10-18 DOI:10.3390/gels10100666
Soukaina El Hajj, Martial Bankoué Ntaté, Cyril Breton, Robin Siadous, Rachida Aid, Magali Dupuy, Didier Letourneur, Joëlle Amédée, Hervé Duval, Bertrand David
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引用次数: 0

摘要

通过骨功能细胞和内皮细胞的体外共培养来了解血液和骨骼之间的相互作用,是揭示骨骼再生治疗潜力的关键因素。此外,采用数值模拟技术评估氧气浓度等局部物理因素和剪切应力等机械刺激,也可为研究提供支持。在这项研究中,我们开发了间充质干细胞系(MSC)和人脐静脉内皮细胞系(HUVEC),它们在三维、多孔、天然拉普兰/葡聚糖支架的流动条件下进行共培养。2 周后,动态条件下的存活率(>94%)高于静态条件下的存活率((
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Bone Spheroid Development Under Flow Conditions with Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells in a 3D Porous Hydrogel Supplemented with Hydroxyapatite.

Understanding the niche interactions between blood and bone through the in vitro co-culture of osteo-competent cells and endothelial cells is a key factor in unraveling therapeutic potentials in bone regeneration. This can be additionally supported by employing numerical simulation techniques to assess local physical factors, such as oxygen concentration, and mechanical stimuli, such as shear stress, that can mediate cellular communication. In this study, we developed a Mesenchymal Stem Cell line (MSC) and a Human Umbilical Vein Endothelial Cell line (HUVEC), which were co-cultured under flow conditions in a three-dimensional, porous, natural pullulan/dextran scaffold that was supplemented with hydroxyapatite crystals that allowed for the spontaneous formation of spheroids. After 2 weeks, their viability was higher under the dynamic conditions (>94%) than the static conditions (<75%), with dead cells central in the spheroids. Mineralization and collagen IV production increased under the dynamic conditions, correlating with osteogenesis and vasculogenesis. The endothelial cells clustered at the spheroidal core by day 7. Proliferation doubled in the dynamic conditions, especially at the scaffold peripheries. Lattice Boltzmann simulations showed negligible wall shear stress in the hydrogel pores but highlighted highly oxygenated zones coinciding with cell proliferation. A strong oxygen gradient likely influenced endothelial migration and cell distribution. Hypoxia was minimal, explaining high viability and spheroid maturation in the dynamic conditions.

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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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