开发用于生物医学应用中可控葡萄糖释放的聚乙烯醇水凝胶。

IF 5 3区 化学 Q1 POLYMER SCIENCE Gels Pub Date : 2024-10-19 DOI:10.3390/gels10100668
Rosa M Quispe-Siccha, Osvaldo I Medina-Sandoval, Abraham Estrada-Tinoco, Jorge A Pedroza-Pérez, Adolfo Martínez-Tovar, Irma Olarte-Carrillo, Rafael Cerón-Maldonado, Arturo Reding-Bernal, Juan C López-Alvarenga
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引用次数: 0

摘要

聚乙烯醇(PVA)水凝胶因其优异的生物物理特性,在制药和生物医学领域有着广泛的应用。本研究的重点是制备和表征胶囊状 PVA 水凝胶,以增强其生物相容性和孔隙率,从而控制葡萄糖释放和细胞增殖。研究人员使用不同浓度(Cs)和分子量(MWs)的 PVA 制备了两种不同长度(A(10 毫米)和 B(20 毫米))的水凝胶,以控制葡萄糖在 60 分钟内的释放。制备过程包括 PVA 凝胶制备和 PVA 水凝胶形成。将水凝胶浸入 37 °C 的生理盐水中,以 500 rpm 的转速搅拌,研究葡萄糖的释放情况。SUP-B15 细胞系生长在六种 A1 水凝胶中,进行生物相容性测试。结果表明,所有水凝胶在 37 °C 下都保持稳定,没有降解。C 值和 MW 值较高的水凝胶具有更致密、更少孔的结构,葡萄糖储存能力较低,断裂伸长率较高。在 60 分钟内,观察到葡萄糖释放、扩散速度和通量存在显著差异,其中 A1 > A4、B1 > B4 和 B1 > A1 的差异更为明显。A1 和 B1 的数值较高,因为它们的孔隙率分布较高,葡萄糖更容易扩散。B1 较大,由于其长度增加,葡萄糖含量也更多。细胞在与水凝胶接触 48 小时后的生长反应和存活率与对照组相似(4.5 × 105 个细胞/毫升,存活率为 98.5%;4.8 × 105 个细胞/毫升,存活率为 99.7%),从而证明了水凝胶的生物相容性。根据孔隙率、C、MW 和长度的不同,水凝胶在 60 分钟内有效地释放了葡萄糖,并与细胞系表现出良好的生物相容性。
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Development of Polyvinyl Alcohol Hydrogels for Controlled Glucose Release in Biomedical Applications.

Polyvinyl alcohol (PVA) hydrogels have a wide range of applications in the pharmaceutical and biomedicine fields due to their exceptional biophysical properties. The study focuses on preparing and characterizing capsule-shaped PVA hydrogels to enhance their biocompatibility and porosity for controlled glucose release and cell proliferation. The hydrogels were prepared using different concentrations (Cs) and molecular weights (MWs) of PVA, with two different lengths, A (10 mm) and B (20 mm), to control glucose release over 60 min. The preparation process involved PVA gel preparation and PVA hydrogel formation. A total of 500 µL of glucose was injected into all dehydrated hydrogels in groups A and B. Glucose release was studied by immersing the hydrogels in saline at 37 °C with stirring at 500 rpm. The SUP-B15 cell line was grown in six A1 hydrogels for biocompatibility testing. The results indicate that all hydrogels remained stable at 37 °C without degrading. Those with a higher C and MW exhibited a denser and less porous structure, lower glucose storage capacity, and higher elongation at break. Significant differences in glucose release, diffusion speed, and flux were observed, which were more evident in A1 > A4, B1 > B4, and B1 > A1 over 60 min. A1 and B1 had higher values because their higher porosity distribution allowed glucose to diffuse more easily. B1, being larger, has more glucose due to its increased length. The cell growth response and viability at 48 h in contact with the hydrogels was similar to that of the control (4.5 × 105 cells/mL, 98.5% vs. 4.8 × 105 cells/mL, 99.7% viability), thus demonstrating biocompatibility. The hydrogels effectively released glucose over 60 min, with variations based on porosity, C, MW, and length, and demonstrated good biocompatibility with the cell line.

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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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