唾液白细胞介素-1β和IL-10在区分牙周健康与牙周炎以及稳定与不稳定牙周炎方面的诊断潜力：病例对照研究。

IF 2.2 Q2 DENTISTRY, ORAL SURGERY & MEDICINE International Journal of Dentistry Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI:10.1155/2024/8006278

Zainab J Raheem, Hayder Raad Abdulbaqi

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摘要

研究目的本病例对照研究旨在探讨唾液白细胞介素（IL）-1β、IL-10和IL-1β/IL-10比值在鉴别牙周炎与牙周健康、稳定型牙周炎与不稳定型牙周炎方面的诊断准确性。研究方法收集 135 名参与者（牙周完好的健康者 45 名[作为健康对照]，稳定型牙周炎 39 名，不稳定型牙周炎 51 名）的唾液样本，然后记录临床牙周参数。采用酶联免疫吸附试验测定唾液中的 IL-1β 和 IL-10 水平。通过估计 IL-1β、IL-10 和 IL-1β/IL-10 的曲线下面积（AUC）、灵敏度和特异性来区分不同组别。结果不稳定牙周炎组唾液 IL-1β 水平（分别为 426 ± 59、247 ± 55 和 204 ± 36 pg/ml [皮克/毫升]）明显高于稳定牙周炎组和健康对照组。虽然对照组唾液 IL-10 水平（360.7 ± 80.5 pg/ml）明显高于不稳定牙周炎组（146.92 ± 1.8 pg/ml），但对照组与稳定牙周炎组（317.04 ± 59.8 pg/ml）之间无明显差异。IL-1β、IL-10和IL-1β/IL-10在区分健康对照组和不稳定型牙周炎方面具有显著的诊断准确性（AUC分别为0.99、0.96和1；灵敏度分别为0.98、1和1；特异性分别为0.95、0.95和1）。同样，它们在区分不稳定型牙周炎和稳定型牙周炎方面也显示出明显的诊断准确性（AUC = 0.98、0.99 和 1；灵敏度 = 0.94、1 和 1；特异性 = 0.94、0.97 和 1）。结论唾液IL-1β、IL-10和IL-1β/IL-10在区分健康对照组和牙周炎以及稳定型和不稳定型牙周炎方面具有很高的潜力。试验注册：临床试验注册：ClinicalTrials.gov identifier：NCT05722613。

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Diagnostic Potential of Salivary Interleukin-1β and IL-10 for Distinguishing Periodontal Health From Periodontitis and Stable From Unstable Periodontitis: A Case-Control Study.

Objective: This case-control study aimed to investigate the diagnostic accuracy of salivary interleukin (IL)-1β, IL-10, and IL-1β/IL-10 ratio to discriminate periodontitis from periodontal health and stable from unstable periodontitis. Methods: Saliva samples were collected from 135 (healthy on an intact periodontium = 45 [as healthy control], stable periodontitis = 39, and unstable periodontitis = 51) participants, and then clinical periodontal parameters were recorded. An enzyme-linked immunosorbent assay was used to determine salivary levels of IL-1β and IL-10. Area under the curves (AUCs), sensitivity, and specificity of IL-1β, IL-10, and IL-1β/IL-10 were estimated to discriminate between groups. Result: The level of salivary IL-1β was significantly higher in unstable periodontitis than in stable periodontitis and healthy control groups (426 ± 59, 247 ± 55, and 204 ± 36 pg/ml [picograms per milliliter], respectively). While the level of salivary IL-10 was significantly higher in the control group (360.7 ± 80.5 pg/ml) than unstable periodontitis group (146.92 ± 1.8 pg/ml), no significant difference was found between the control and stable periodontitis (317.04 ± 59.8 pg/ml) groups. IL-1β, IL-10, and IL-1β/IL-10 had significant diagnostic accuracy for differentiating healthy control from unstable periodontitis (AUCs = 0.99, 0.96, and 1; sensitivity = 0.98,1, and 1; specificity = 0.95, 0.95, and 1, respectively). Similarly, they showed significant diagnostic accuracy in distinguishing unstable from stable periodontitis (AUCs = 0.98, 0.99, and 1; sensitivity = 0.94, 1, and 1; specificity = 0.94, 0.97, and 1, respectively). Conclusion: Salivary IL-1β, IL-10, and IL-1β/IL-10 have a high potential to discriminate healthy control from periodontitis and stable from unstable periodontitis. Trial Registration: ClinicalTrials.gov identifier: NCT05722613.