在菠菜(Spinacia oleracea L.)中使用新型植物生长促进菌株枯草芽孢杆菌 NR5 缓解砷胁迫。

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-10-22 DOI:10.1002/jobm.202400401
Khan M Sarim, Renu Shukla, Manish S Bhoyar, Baljeet Kaur, Dhananjay P Singh
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引用次数: 0

摘要

本研究旨在鉴定可用于缓解砷胁迫的抗砷(As)细菌菌株。研究人员从印度马哈拉施特拉邦纳格河分离出了耐砷极限为 1100 毫克/升的枯草芽孢杆菌 NR5。它还具有促进植物生长(PGP)的特性,如磷酸盐溶解、嗜苷酸、氨和硝酸盐还原,以及抗生素耐受性。此外,扫描电子显微镜(SEM)和透射电子显微镜(TEM)表明,生物吸附是砷耐受性的可能机制。傅立叶变换红外光谱在 3379.0 处出现了一个强峰,对应于砷处理过的 NR5 中的胺,这也表明金属与细胞表面蛋白发生了相互作用。NR5 中砷还原酶基因的扩增进一步表明了砷在细胞内的转化。此外,NR5 在菠菜植物中的耐砷能力也得到了证实,该细菌通过产生与防御相关的脯氨酸分子,有效缓解了 25 ppm 的砷浓度。扫描电子显微镜(SEM)、电子显微镜(TEM)和傅立叶变换红外光谱(FTIR)的证据表明,生物吸附可能是 NR5 耐受砷和砷转化的主要机制。具有 PGP 特性、高砷耐受性和抗生素耐受性的 B. mycoides NR5 增强了菠菜植物对砷的耐受性,因此该菌株可作为受污染农业土壤和水体砷生物修复的更好选择。
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Arsenic Stress Mitigation Using a Novel Plant Growth-Promoting Bacterial Strain Bacillus mycoides NR5 in Spinach Plant (Spinacia oleracea L.).

Present study aimed to identify arsenic (As)-resistant bacterial strains that can be used to mitigate arsenic stress. A bacterium Bacillus mycoides NR5 having As tolerance limit of 1100 mg L-1 was isolated from Nag River, Maharashtra, India. It was also equipped with plant growth-promoting (PGP) attributes like phosphate solubilization, siderophores, ammonia, and nitrate reduction, with added antibiotic tolerance. Furthermore, scanning electron microscopy (SEM) and transmission electron micrograph (TEM) suggested biosorption as possible mechanisms of arsenic tolerance. A strong peak in FTIR spectra at 3379.0 corresponding to amine in As-treated NR5 also indicated metal interaction with cell surface protein. Amplification of arsenic reductase gene in NR5 further suggested intracellular transformation of As speciation. Moreover, As tolerance capability of NR5 was shown in spinach plants in which the bacterium effectively mitigated 25 ppm As by producing defense-related proline molecules. Evidence from SEM, TEM, and FTIR, concluded biosorption possibly the primary mechanism of As tolerance in NR5 along with the transformation of arsenic. B. mycoides NR5 with PGP attributes, high As tolerance, and antibiotic resistance mediated enhanced As tolerance in spinach plants advocated that the strain can be a better choice for As bioremediation in contaminated agricultural soil and water.

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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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