探索金纳米粒子的体外抗生物膜潜力和体内毒性评估。

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-10-28 DOI:10.1002/jobm.202400329
Iram Liaqat, Saiqa Andleeb, Sajida Naseem, Abid Ali, Asma Abdul Latif, Muhamad Nauman Aftab, Sikander Ali, Asia Bibi, Muhammad Mubin, Awais Khalid, Muhammad Afzaal, Guo-Jing Yang, Shahzad Tufail, Haroon Ahmad
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引用次数: 0

摘要

本研究首先通过紫外可见光谱、扫描电子显微镜、XRD 和傅立叶变换红外光谱对生物合成的 AuNPs 进行了表征,然后利用小鼠模型对其进行了毒性评估。生物合成 AuNPs 的紫外可见光谱在 540-549 nm 处出现峰值,而傅立叶变换红外光谱则显示出各种官能团,包括 O-H、酰胺 I、酰胺 II、O-H、C-H 基团等。扫描电子显微镜(SEM)显示其尺寸变化范围为 30 至 60 纳米。针对病原体分离物的抗菌潜力显示,铜绿假单胞菌 AuNPs 的 ZOI 较大(31.0 mm)。在 90 µg mL-1 AuNPs 浓度下,抗生物膜活性的抑制率高达 100%。毒性评估显示半数致死剂量为 70 毫克/千克。接触 AuNPs 会导致血清 AST 水平发生显著变化(p -1 of AuNPs exposure)。雄性白化小鼠肾脏和肝脏的组织病理学显示,暴露于最高浓度 AuNPs 的小鼠出现坏死、细胞变形和肝细胞脱落。本研究表明,生物合成的 AuNPs 具有有效的抗菌和抑制生物膜的潜力。即使在较低浓度下,AuNPs 也具有很强的杀菌作用,这表明 AuNPs 在抗击病原体方面具有很好的潜力。总之,纳米技术可能会通过开发创新药物彻底改变人类生活和医疗行业,并有可能在更短的时间内非侵入性地治疗疾病。因此,体外生物安全性和实验观察以及体内结果对于将新型疗法转化为医疗实践至关重要,从而进一步推动其未来的发展。
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Exploring In Vitro Antibiofilm Potential and In Vivo Toxicity Assessment of Gold Nanoparticles.

In this study, biogenically synthesized AuNPs were first characterized via UV visible spectroscopy, SEM, XRD, and FTIR followed by toxicity evaluation using mice model. UV-visible spectroscopy of biogenic AuNPs showed peaks at 540-549 nm, while FTIR spectrum showed various functional groups involving O-H, Amide I, Amide II, O-H, C-H groups, and so on. SEM showed the size variation from 30 to 60 nm. Antibacterial potential against pathogenic isolates showed bigger ZOI (31.0 mm) against Pseudomonas aeruginosa AuNPs. Antibiofilm activity showing up to 100% inhibition at 90 µg mL-1 concentration of AuNPs. Toxicity evaluation showed LD50 as 70 mg kg-1. Exposure to AuNPs caused significant changes in the levels of serum AST (p < 0.05) at 100-150 mg kg-1 of AuNPs exposure. Histopathology of male albino mice kidney and liver revealed that mice exposed to maximum concentration of AuNPs showed necrosis, cell distortion, and hepatocytes detachment. Present study showed that biologically synthesized AuNPs possess effective antimicrobial and biofilm inhibitory potential. AuNPs strong bactericidal effect even at lower concentration suggest that NPs could have excellent potential for combating pathogens. In conclusion, nanotechnology may revolutionize human life and medical industry by developing innovative drugs with the potential to treat diseases in shorter and noninvasive time period. Hence, in vitro biosafety and experimental observations followed by in vivo outcomes are crucial in shifting the novel therapeutics into medical practice thus leading further into their future development.

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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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