Jie Gao, Chen Wang, Pei-Cong Tian, Chuang Liu, Taswar Ahsan, Yi Wei, Yu-Qian Huang, Shi-Hong Zhang
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The endophytic fungus P. indica affected the physiological characteristics of the host plant by colonizing the plant roots, thereby conferring greater resistance to drought stress. This fungus strongly colonized the roots of peanuts and was found to enhance root activity after 24 h of P. indica colonization under PEG6000. Catalase (CAT) and peroxidase (POD) activities were increased at 24 h in peanut leaves colonized with P. indica. Expression of drought-related genes, such as AhNCED1, AhP5CS, and DREB2A was upregulated at 24 h of P. indica colonization. In addition, after PEG6000 treatment, proline, soluble protein, and abscisic acid (ABA) concentrations in plants were increased, while the accumulation of malondialdehyde (MDA), and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was decreased in P. indica colonized peanut. In conclusion, P. indica mediated peanut plant protection against the detrimental effects of drought resulted from enhanced antioxidant enzyme activities, and the upregulated expression of drought-related genes for lower membrane damage.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400305"},"PeriodicalIF":3.5000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Peanut-Colonized Piriformospora indica Enhanced Drought Tolerance by Modulating the Enzymes and Expression of Drought-Related Genes.\",\"authors\":\"Jie Gao, Chen Wang, Pei-Cong Tian, Chuang Liu, Taswar Ahsan, Yi Wei, Yu-Qian Huang, Shi-Hong Zhang\",\"doi\":\"10.1002/jobm.202400305\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Peanut (Arachis hypogaea L.) is an important cash and oil seed crop, mostly distributed in arid and semi-arid areas. 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Expression of drought-related genes, such as AhNCED1, AhP5CS, and DREB2A was upregulated at 24 h of P. indica colonization. In addition, after PEG6000 treatment, proline, soluble protein, and abscisic acid (ABA) concentrations in plants were increased, while the accumulation of malondialdehyde (MDA), and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was decreased in P. indica colonized peanut. 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引用次数: 0
摘要
花生(Arachis hypogaea L.)是一种重要的经济作物和油料作物,主要分布在干旱和半干旱地区。近年来,受大气环流异常等因素的影响,我国干旱频发,且日趋严重。这给花生生产带来了严峻挑战。本研究旨在探讨内生真菌 Piriformospora indica 与花生植株形成共生关系的潜力,并评估 P. indica 定殖的花生植株在 20% 聚乙二醇 6000(PEG6000)的模拟干旱胁迫处理下的耐旱性。内生真菌 P. indica 通过在寄主植物根部定殖影响了寄主植物的生理特性,从而增强了对干旱胁迫的抵抗力。在 PEG6000 条件下,这种真菌在花生根部的定殖作用很强,在 P. indica 定殖 24 小时后,根系活性得到增强。用 P. indica 定殖的花生叶片在 24 小时后过氧化氢酶(CAT)和过氧化物酶(POD)活性增加。在 P. indica 定殖 24 小时后,干旱相关基因(如 AhNCED1、AhP5CS 和 DREB2A)的表达上调。此外,经过 PEG6000 处理后,植株中的脯氨酸、可溶性蛋白和脱落酸(ABA)浓度增加,而 P. indica 定殖花生中丙二醛(MDA)和过氧化氢(H2O2)的积累减少。总之,P. indica 能增强抗氧化酶的活性,并上调干旱相关基因的表达,从而降低膜损伤,从而保护花生植物免受干旱的不利影响。
Peanut-Colonized Piriformospora indica Enhanced Drought Tolerance by Modulating the Enzymes and Expression of Drought-Related Genes.
Peanut (Arachis hypogaea L.) is an important cash and oil seed crop, mostly distributed in arid and semi-arid areas. In recent years, due to the influence of atmospheric circulation anomalies and other factors, drought has become frequent and increasingly serious in China. This has posed serious challenges to peanut production. The objective of this study was to investigate the potential of the endophytic fungus Piriformospora indica to form a symbiotic relationship with peanut plants and to evaluate the drought tolerance of P. indica-colonized peanut plants subjected to a simulated drought stress treatment using 20% polyethylene glycol 6000 (PEG6000). The endophytic fungus P. indica affected the physiological characteristics of the host plant by colonizing the plant roots, thereby conferring greater resistance to drought stress. This fungus strongly colonized the roots of peanuts and was found to enhance root activity after 24 h of P. indica colonization under PEG6000. Catalase (CAT) and peroxidase (POD) activities were increased at 24 h in peanut leaves colonized with P. indica. Expression of drought-related genes, such as AhNCED1, AhP5CS, and DREB2A was upregulated at 24 h of P. indica colonization. In addition, after PEG6000 treatment, proline, soluble protein, and abscisic acid (ABA) concentrations in plants were increased, while the accumulation of malondialdehyde (MDA), and hydrogen peroxide (H2O2) was decreased in P. indica colonized peanut. In conclusion, P. indica mediated peanut plant protection against the detrimental effects of drought resulted from enhanced antioxidant enzyme activities, and the upregulated expression of drought-related genes for lower membrane damage.
期刊介绍:
The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions.
Papers published deal with:
microbial interactions (pathogenic, mutualistic, environmental),
ecology,
physiology,
genetics and cell biology/development,
new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications)
novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).