用于胶质瘤生存风险分层和预后的巨噬细胞极化相关基因特征。

Weiming Zhong, Kaifen Xiong, Shuwang Li, Chuntao Li
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引用次数: 0

摘要

巨噬细胞极化在肿瘤免疫细胞浸润和肿瘤生长中起着至关重要的作用。在这项研究中,我们选择了一系列区分 M1 和 M2 巨噬细胞的基因,并探讨了它们在胶质瘤中的预后价值。我们的研究共纳入了 170 个基因。我们使用 CGGA 数据库作为训练队列,使用 TCGA 数据库作为验证队列。通过 GO 和 KEGG 分析确定了基因的生物学过程和功能。采用 Kaplan-Meier 分析比较不同组间的生存率差异。重要的是,我们根据 CGGA 使用 Cox 回归分析建立了一个风险评分模型,并在 TCGA 数据库和我们的测序数据中进行了验证。高危组中的胶质瘤患者与高病理分级、IDH WT状态、MGMT启动子未甲基化、1p19q非编码缺失有关,且易出现不良预后。GEPIA结果显示,CD300C、CNRIP1和MYO1F是与免疫浸润最相关的基因。q-RT-PCR 证实了这些基因在低级别胶质瘤和胶质母细胞瘤之间的差异表达。巨噬细胞极化相关基因特征可以预测胶质瘤患者的恶性程度和预后,并有可能成为未来胶质瘤免疫治疗的靶点。
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Macrophage polarization-related gene signature for risk stratification and prognosis of survival in gliomas

Macrophage polarization plays an essential role in tumour immune cell infiltration and tumour growth. In this study, we selected a series of genes distinguishing between M1 and M2 macrophages and explored their prognostic value in gliomas. A total of 170 genes were included in our study. The CGGA database was used as the training cohort and the TCGA database as the validation cohort. The biological processes and functions were identified by GO and KEGG analysis. Kaplan–Meier analysis was used to compare survival differences between groups. Importantly, we built a risk score model using Cox regression analysis based on the CGGA and verified it in the TCGA database and our sequencing data. Patients with gliomas in the high-risk group were associated with high pathologic grade, IDH WT status, MGMT promoter unmethylation, 1p19q non-codeletion and prone to have a poor outcome. GEPIA results revealed that CD300C, CNRIP1 and MYO1F are the most related genes of immune infiltrations. The differential expression of these genes between low-grade gliomas and glioblastomas was confirmed by q-RT-PCR. Macrophage polarization-related gene signatures can predict the malignancy and outcome of patients with gliomas and might act as a promising target for glioma immunotherapy in the future.

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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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