通过分析头部空间挥发性有机化合物识别病毒感染。

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2024-10-30 DOI:10.1088/1752-7163/ad89f0
E Sanmark, P Marjanen, J Virtanen, K Aaltonen, S Tauriainen, P Österlund, M Mäkelä, S Saari, A Roine, T Rönkkö, V A Vartiainen
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引用次数: 0

摘要

背景:人体呼吸细胞产生的挥发性有机化合物(VOCs)反映了新陈代谢和病理生理过程,可利用现代技术进行检测。对呼出的气体或室内空气进行分析,有可能在未来上呼吸道感染(如 COVID-19 或流感)的筛查中发挥重要作用:在这项实验研究中,收集并分析了被选定病原体(甲型 H1N1 流感、季节性冠状病毒 OC43 和 NL63)感染的体外细胞培养物的顶空空气样本。采用实时质子转移反应飞行时间质谱仪和差分迁移率光谱仪测量挥发性有机化合物。测量每 12 小时进行一次,持续 7 天。未感染细胞和细胞培养基作为参照物:在 H1N1 和 OC43 中,我们观察到四种不同的挥发性有机化合物,它们在感染期间达到峰值。在这两种感染中还观察到了不同的单个 VOC。在所有分析中,活性在两天后开始明显增加。在感染 NL63 的细胞中,我们没有发现 VOC 生成的增加:VOC 分析似乎适用于区分受感染细胞与未感染细胞,以及不同病毒与其他病毒。未来,这在个体诊断和室内环境筛选方面都有实用价值。
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Identifying viral infections through analysis of head space volatile organic compounds.

Volatile organic compounds (VOCs) produced by human respiratory cells reflect metabolic and pathophysiological processes which can be detected with the use of modern technology. Analysis of exhaled breath or indoor air may potentially play an important role in screening of upper respiratory tract infections such as COVID-19 or influenza in the future. In this experimental study, air samples were collected and analyzed from the headspace of anin vitrocell culture infected by selected pathogens (influenza A H1N1 and seasonal coronaviruses OC43 and NL63). VOCs were measured with a real-time proton-transfer-reaction time-of-flight mass spectrometer and a differential mobility spectrometer. Measurements were performed every 12 h for 7 d. Non-infected cells and cell culture media served as references. In H1N1 and OC43 we observed four different VOCs which peaked during the infection. Different, individual VOCs were also observed in both infections. Activity began to clearly increase after 2 d in all analyses. We did not see increased VOC production in cells infected with NL63. VOC analysis seems to be suitable to differentiate the infected cells from those which are not infected as well as different viruses, from another. In the future, this could have practical value in both individual diagnostics and indoor environment screening.

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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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