填充了犬胎盘水凝胶和间充质干细胞的 PLLA/GO 支架用于山羊下颌骨的骨修复。

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2024-10-20 DOI:10.3390/jfb15100311
Thamires Santos-Silva, Inácio Silva Viana, Andrea Barros Piazzon S Queiroz, Fabrício Singaretti de Oliveira, Bianca de Oliveira Horvath-Pereira, Leandro Norberto da Silva-Júnior, Michelle Silva Araujo, Paulo Alescio Canola, Luís Gustavo Gosuen G Dias, Marcelo Melo Soares, Maria Angelica Miglino
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引用次数: 0

摘要

动物骨骼缺损的原因多种多样,包括疾病、肿瘤和最常见的创伤。超过临界尺寸的粉碎性骨折可能会因血管生成不足或中断而愈合不良,导致骨再生所需的祖细胞数量不足。在这种情况下,使用聚左旋乳酸/氧化石墨烯(PLLA/GO)的3D打印技术旨在通过创建结合犬胎盘水凝胶和间充质干细胞的定制支架来解决这一问题,与使用钛板固定的对照组相比,该技术可用于山羊下颌骨。对十个犬胎盘进行脱细胞处理,并使用组织学技术进行鉴定。生产了一种源自犬胎盘细胞外基质(cpECM)的水凝胶,以改善细胞对支架的附着。体外细胞毒性和细胞对 cpECM 水凝胶的附着情况通过扫描电子显微镜(SEM)进行了评估。最终制成的生物材料(cpECM 水凝胶和 PLLA/GO 支架)保持了其功能结构,并支持体外细胞粘附、维持和增殖。热成像显示,含有 cpECM 水凝胶的 PLLA/GO 支架的性能与对照组相似。计算机断层扫描显示了骨胼胝,表明修复过程正在进行中。这些研究结果证明了这些材料在外科手术中的创新技术潜力。未来对 PLLA/GO 支架的研究将进一步揭示它们对山羊模型的影响。
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PLLA/GO Scaffolds Filled with Canine Placenta Hydrogel and Mesenchymal Stem Cells for Bone Repair in Goat Mandibles.

Bone defects in animals can arise from various causes, including diseases, neoplasms, and most commonly, trauma. Comminuted fractures that exceed the critical size may heal poorly due to deficient or interrupted vascularization, resulting in an insufficient number of progenitor cells necessary for bone regeneration. In this context, 3D printing techniques using poly-L-lactic acid/graphene oxide (PLLA/GO) aim to address this issue by creating customized scaffolds combined with canine placenta hydrogel and mesenchymal stem cells for use in goat mandibles, compared to a control group using titanium plate fixation. Ten canine placentas were decellularized and characterized using histological techniques. A hydrogel derived from the canine placenta extracellular matrix (cpECM) was produced to improve cell attachment to the scaffolds. In vitro cytotoxicity and cell adhesion to the cpECM hydrogel were assessed by scanning electron microscopy (SEM). The resulting biomaterials, cpECM hydrogel and PLLA/GO scaffolds, maintained their functional structure and supported cell adhesion, maintenance, and proliferation in vitro. Thermography showed that PLLA/GO scaffolds with cpECM hydrogel performed effectively, similar to the control group. Computed tomography scans revealed bone calluses, suggesting an ongoing repair process. These findings demonstrate the innovative technological potential of these materials for use in surgical interventions. Future studies on PLLA/GO scaffolds will provide further insights into their effects on goat models.

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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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