Linghan Xie, Bin Zhang, Qiang Chen, Hangyu Ji, Ji Chen, Zanli Jiang, Lei Zhu, Xiaotao Wu
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引用次数: 0
摘要
研究目的本研究旨在评估电针刺激迷走神经（EAS）对脊髓损伤（SCI）大鼠模型炎症反应的影响：方法：采用改良艾伦法建立成年雄性 Sprague Dawley 大鼠 T10 SCI 模型。EAS组接受大鼠耳甲迷走神经治疗，SCI组不接受任何EAS治疗。苏木精-伊红染色反映了炎症浸润的程度。用酶联免疫吸附试验检测脊髓组织、脑脊液炎症和外周血中的炎症细胞因子。用免疫荧光法评估星形胶质细胞和小胶质细胞的变化：结果：电针刺激治疗可抑制炎症浸润以及白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α、星形胶质细胞和小胶质细胞的浓度。多次EAS治疗对SCI炎症有明显影响：结论：单次 EAS 治疗对炎症的影响有限，但多次治疗对炎症有明显的抑制作用。
Effect of Electrical Stimulation of the Vagus Nerve on Inflammation in Rats With Spinal Cord Injury.
Objective: The purpose of this study was to assess the effect of electroacupuncture stimulation (EAS) of the vagus nerve on the inflammatory response in rat models of spinal cord injury (SCI).
Methods: The T10 SCI model in adult male Sprague Dawley rats was established using the modified Allen's method. The EAS group was treated with the therapy on the vagus nerve of rat ear nails, while the SCI group did not receive any EAS treatment. The degree of inflammatory infiltration was reflected by hematoxylin-eosin staining. The inflammatory cytokines in spinal cord tissues, cerebrospinal fluid inflammation, and peripheral blood were detected by enzyme-linked immunosorbent assay. Changes in astrocytes and microglia were assessed using an immunofluorescence assay.
Results: Electroacupuncture stimulation treatment inhibited inflammatory infiltration, as well as the concentrations of interleukin-6, interleukin-1β, tumor necrosis factor-α, astrocytes, and microglia at 1, 6, and 24 hours after 1 EAS treatment. Multiple EAS treatments had an obvious effect on SCI inflammation.
Conclusion: A single EAS treatment had a limited effect on inflammation, but multiple treatments had a significant inhibitory effect on inflammation.
期刊介绍：
The Journal of Manipulative and Physiological Therapeutics (JMPT) is an international and interdisciplinary journal dedicated to the advancement of conservative health care principles and practices. The JMPT is the premier biomedical publication in the chiropractic profession and publishes peer reviewed, research articles and the Journal''s editorial board includes leading researchers from around the world.
The Journal publishes original primary research and review articles of the highest quality in relevant topic areas. The JMPT addresses practitioners and researchers needs by adding to their clinical and basic science knowledge and by informing them about relevant issues that influence health care practices.
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