{"title":"中国欧亚禾花鼠（Micromys minutus）Pallas 1771 完整线粒体基因组的测序和系统发育分析。","authors":"Hong-Yan Wang, Yu-Qi Wu, Zhi-Hui Zhang, Zhu Liu","doi":"10.1080/23802359.2024.2417974","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to examine the complete mitochondrial genome sequence of the Eurasian Harvest Mouse (<i>Micromys minutus</i>) through polymerase chain reaction. The mitochondrial genome of <i>M. minutus</i> was characterized as a circular, double-stranded DNA molecule, encompassing a total length of 16,239 bp. The mitochondrial genome composition of <i>M. minutus</i> was found to include 13 protein-coding genes, a single control region, 22 tRNA genes, 2 rRNA genes, and a origin of L-strand replication. The accurate identification, sequencing, and assembly of the <i>M. minutus</i> mitochondrial genome were successfully conducted in this study, providing a resource for the scientific community to accurately attribute the mitochondrial genome of this species.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500544/pdf/","citationCount":"0","resultStr":"{\"title\":\"Sequencing and phylogenetic analysis of the complete mitochondrial genome of the Eurasian Harvest Mouse (<i>Micromys minutus</i>) Pallas 1771 from China.\",\"authors\":\"Hong-Yan Wang, Yu-Qi Wu, Zhi-Hui Zhang, Zhu Liu\",\"doi\":\"10.1080/23802359.2024.2417974\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to examine the complete mitochondrial genome sequence of the Eurasian Harvest Mouse (<i>Micromys minutus</i>) through polymerase chain reaction. The mitochondrial genome of <i>M. minutus</i> was characterized as a circular, double-stranded DNA molecule, encompassing a total length of 16,239 bp. The mitochondrial genome composition of <i>M. minutus</i> was found to include 13 protein-coding genes, a single control region, 22 tRNA genes, 2 rRNA genes, and a origin of L-strand replication. The accurate identification, sequencing, and assembly of the <i>M. minutus</i> mitochondrial genome were successfully conducted in this study, providing a resource for the scientific community to accurately attribute the mitochondrial genome of this species.</p>\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2024-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500544/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/23802359.2024.2417974\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/23802359.2024.2417974","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在通过聚合酶链式反应检测欧亚禾花鼠（Micromys minutus）的完整线粒体基因组序列。禾花鼠的线粒体基因组为环状双链 DNA 分子，总长度为 16,239 bp。研究发现，M. minutus 的线粒体基因组包括 13 个蛋白质编码基因、一个控制区、22 个 tRNA 基因、2 个 rRNA 基因和一个 L 链复制起源。这项研究成功地完成了M. minutus线粒体基因组的准确鉴定、测序和组装，为科学界准确归属该物种的线粒体基因组提供了资源。
Sequencing and phylogenetic analysis of the complete mitochondrial genome of the Eurasian Harvest Mouse (Micromys minutus) Pallas 1771 from China.
This study aimed to examine the complete mitochondrial genome sequence of the Eurasian Harvest Mouse (Micromys minutus) through polymerase chain reaction. The mitochondrial genome of M. minutus was characterized as a circular, double-stranded DNA molecule, encompassing a total length of 16,239 bp. The mitochondrial genome composition of M. minutus was found to include 13 protein-coding genes, a single control region, 22 tRNA genes, 2 rRNA genes, and a origin of L-strand replication. The accurate identification, sequencing, and assembly of the M. minutus mitochondrial genome were successfully conducted in this study, providing a resource for the scientific community to accurately attribute the mitochondrial genome of this species.
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