{"title":"缩小差距:纳米材料危害评估的创新性人体体外方法及其在安全和可持续设计、风险评估和生命周期评估中的作用。","authors":"","doi":"10.1016/j.impact.2024.100533","DOIUrl":null,"url":null,"abstract":"<div><div>The application of nanomaterials in industry and consumer products is growing exponentially, which has pressed the development and use of predictive human <em>in vitro</em> models in pre-clinical analysis to closely extrapolate potential toxic effects <em>in vivo</em>. The conventional cytotoxicity investigation of nanomaterials using cell lines from cancer origin and culturing them two-dimensionally in a monolayer without mimicking the proper pathophysiological microenvironment may affect a precise prediction of <em>in vitro</em> effects at <em>in vivo</em> level. In recent years, complex <em>in vitro</em> models (also belonging to the new approach methodologies, NAMs) have been established in unicellular to multicellular cultures either by using cell lines, primary cells or induced pluripotent stem cells (iPSCs), and reconstituted into relevant biological dimensions mimicking <em>in vivo</em> conditions. These advanced <em>in vitro</em> models retain physiologically reliant exposure scenarios particularly appropriate for oral, dermal, respiratory, and intravenous administration of nanomaterials, which have the potential to improve the <em>in vivo</em> predictability and lead to reliable outcomes. In this perspective, we discuss recent developments and breakthroughs in using advanced human <em>in vitro</em> models for hazard assessment of nanomaterials. We identified fit-for-purpose requirements and remaining challenges for the successful implementation of <em>in vitro</em> data into nanomaterials Safe and Sustainable by Design (SSbD), Risk Assessment (RA), and Life Cycle Assessment (LCA). By addressing the gap between <em>in vitro</em> data generation and the utility of <em>in vitro</em> data for nanomaterial safety assessments, a prerequisite for SSbD approaches, we outlined potential key areas for future development.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bridging the gap: Innovative human-based in vitro approaches for nanomaterials hazard assessment and their role in safe and sustainable by design, risk assessment, and life cycle assessment\",\"authors\":\"\",\"doi\":\"10.1016/j.impact.2024.100533\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The application of nanomaterials in industry and consumer products is growing exponentially, which has pressed the development and use of predictive human <em>in vitro</em> models in pre-clinical analysis to closely extrapolate potential toxic effects <em>in vivo</em>. The conventional cytotoxicity investigation of nanomaterials using cell lines from cancer origin and culturing them two-dimensionally in a monolayer without mimicking the proper pathophysiological microenvironment may affect a precise prediction of <em>in vitro</em> effects at <em>in vivo</em> level. In recent years, complex <em>in vitro</em> models (also belonging to the new approach methodologies, NAMs) have been established in unicellular to multicellular cultures either by using cell lines, primary cells or induced pluripotent stem cells (iPSCs), and reconstituted into relevant biological dimensions mimicking <em>in vivo</em> conditions. These advanced <em>in vitro</em> models retain physiologically reliant exposure scenarios particularly appropriate for oral, dermal, respiratory, and intravenous administration of nanomaterials, which have the potential to improve the <em>in vivo</em> predictability and lead to reliable outcomes. In this perspective, we discuss recent developments and breakthroughs in using advanced human <em>in vitro</em> models for hazard assessment of nanomaterials. We identified fit-for-purpose requirements and remaining challenges for the successful implementation of <em>in vitro</em> data into nanomaterials Safe and Sustainable by Design (SSbD), Risk Assessment (RA), and Life Cycle Assessment (LCA). By addressing the gap between <em>in vitro</em> data generation and the utility of <em>in vitro</em> data for nanomaterial safety assessments, a prerequisite for SSbD approaches, we outlined potential key areas for future development.</div></div>\",\"PeriodicalId\":18786,\"journal\":{\"name\":\"NanoImpact\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"NanoImpact\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452074824000430\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"NanoImpact","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452074824000430","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Bridging the gap: Innovative human-based in vitro approaches for nanomaterials hazard assessment and their role in safe and sustainable by design, risk assessment, and life cycle assessment
The application of nanomaterials in industry and consumer products is growing exponentially, which has pressed the development and use of predictive human in vitro models in pre-clinical analysis to closely extrapolate potential toxic effects in vivo. The conventional cytotoxicity investigation of nanomaterials using cell lines from cancer origin and culturing them two-dimensionally in a monolayer without mimicking the proper pathophysiological microenvironment may affect a precise prediction of in vitro effects at in vivo level. In recent years, complex in vitro models (also belonging to the new approach methodologies, NAMs) have been established in unicellular to multicellular cultures either by using cell lines, primary cells or induced pluripotent stem cells (iPSCs), and reconstituted into relevant biological dimensions mimicking in vivo conditions. These advanced in vitro models retain physiologically reliant exposure scenarios particularly appropriate for oral, dermal, respiratory, and intravenous administration of nanomaterials, which have the potential to improve the in vivo predictability and lead to reliable outcomes. In this perspective, we discuss recent developments and breakthroughs in using advanced human in vitro models for hazard assessment of nanomaterials. We identified fit-for-purpose requirements and remaining challenges for the successful implementation of in vitro data into nanomaterials Safe and Sustainable by Design (SSbD), Risk Assessment (RA), and Life Cycle Assessment (LCA). By addressing the gap between in vitro data generation and the utility of in vitro data for nanomaterial safety assessments, a prerequisite for SSbD approaches, we outlined potential key areas for future development.
期刊介绍:
NanoImpact is a multidisciplinary journal that focuses on nanosafety research and areas related to the impacts of manufactured nanomaterials on human and environmental systems and the behavior of nanomaterials in these systems.