NanoImpact最新文献

Vanadium dioxide (VO₂) is an excellent phase transition material widely used in various applications, and thus inevitably enters the environment via different routes and encounters various organisms. Nonetheless, limited information is available on the environmental hazards of VO₂. In this study, we investigated the impact of two commercial VO₂ particles, nanosized S-VO₂ and micro-sized M-VO₂ on the white rot fungus Phanerochaete chrysosporium. The growth of P. chrysosporium is significantly affected by VO₂ particles, with S-VO₂ displaying a higher inhibitory effect on weight gain. In addition, VO₂ at high concentrations inhibits the formation of fungal fibrous hyphae and disrupts the integrity of fungus cells as evidenced by the cell membrane damage and the loss of cytoplasm. Notably, at 200 μg/mL, S-VO₂ completely alters the morphology of P. chrysosporium, while the M-VO₂ treatment does not affect the mycelium formation of P. chrysosporium. Additionally, VO₂ particles inhibit the laccase activity secreted by P. chrysosporium, and thus prevent the dye decoloration and sawdust decomposition by P. chrysosporium. The mechanism underlying this toxicity is related to the dissolution of VO₂ and the oxidative stress induced by VO₂. Overall, our findings suggest that VO₂ nanoparticles pose significant environmental hazards and risks to white rot fungi.

Most studies on nanoplastics (NPs) focus on aquatic environments, overlooking their combined bioaccumulation with pollutants in terrestrial ecosystems. This study addresses a part of this gap by investigating how polystyrene nanoplastics (PS-NPs) affect the bioaccumulation and translocation of lead (Pb) in Hordeum vulgare L. plants. Using the RHIZOtest device for precise soil contamination control, we quantified PS-NPs (50 nm) in plant shoots via pyrolysis-gas chromatography/mass spectrometry (Py-GCMS) after plant KOH digestion. Our findings revealed that PS-NPs reduce Pb bioaccumulation and make adsorbed Pb onto PS-NPs less bioavailable to plants. For the highest Pb concentration, the Pb uptake index (PUI) followed the trend: Free Pb > NPs + Pb > Pb primary adsorbed by NPs, showing reduced Pb translocation to shoots in the presence of PS-NPs. Moreover, the presence of Pb decreased the bioavailability of PS-NPs probably in response to PS-NPs aggregation or modified charge. The PS-NPs concentrations in shoots range from 275.2 to 400 μg g⁻¹, representing 3.9 to 5.75% of the total PS-NPs. This study highlights the intricate interactions between nanoplastics and metals in soil-plant systems and emphasizes the need for further research on their combined effects and potential risks to food safety.

Anthropogenic activities have negatively impacted the ecosystem dramatically over the last few decades. The environment is becoming more contaminated with heavy metals, pesticides, and microplastics (MPs) as a result of the swift rise in industrialization and urbanisation. These contaminants are present everywhere in the ecosystem, affecting every living creature, from aquatic to terrestrial to aerial. Recently, the widespread of microplastics in the environment has raised serious concerns about the contamination of honey bees by these tiny particles of plastic. Honeybees are the major pollinators which contributes in the pollination of about 70% food that we consume. This review summarizes current research findings on the presence, uptake, and possible effects of microplastics on honey bees. Findings revealed the presence of microplastics in various honey bee matrices, such as honey, pollen, beeswax, and bee bodies, highlighting the potential routes of exposure for these vital pollinators. Additionally, evidence suggests that microplastics can accumulate in honey bee tissues (brain, midgut, Malpighian tubules, trachea, and haemolymph) potentially leading to adverse effects on honey bee health, behaviour, and colony dynamics. Additionally, MPs has a synergistic impact on immune system as well. Change in cuticle profile, reduction in body weight, and changes in eating frequency can regulate overall success rate of their survival. However, significant knowledge gaps remain regarding the long-term consequences for honey bee populations and ecosystem health, which cannot unveil the ultimate degree of future threats. Future research efforts should focus on investigating the interactions between microplastics and other stressors, such as pesticides and pathogens, and assessing the broader ecological implications of honey bee contamination with microplastics. Addressing these knowledge gaps is essential for developing effective mitigation strategies to minimize the impact of microplastics on honey bee populations and safeguarding their vital role in ecosystem functioning and food security.

Nanomaterials are increasingly used in many applications due to their enhanced properties. To ensure their safety for humans and the environment, nanomaterials need to be evaluated for their potential risk. The risk assessment analysis on the nanomaterials based on animal or in vivo studies is accompanied by several concerns, including animal welfare, time and cost needed for the studies. Therefore, incorporating in vitro studies in the risk assessment process is increasingly considered. To be able to analyze the potential risk of nanomaterial to human health, there are factors to take into account. Utilizing in vitro data in the risk assessment analysis requires methods that can be used to translate in vitro data to predict in vivo phenomena (in vitro-in vivo extrapolation (IVIVE) methods) to be incorporated, to obtain a more accurate result. Apart from the experiments and species conversion (for example, translation between the cell culture, animal and human), the challenge also includes the unique properties of nanomaterials that might cause them to behave differently compared to the same materials in a bulk form. This overview presents the IVIVE techniques that are developed to extrapolate pharmacokinetics data or doses. A brief example of the IVIVE methods for chemicals is provided, followed by a more detailed summary of available IVIVE methods applied to nanomaterials. The IVIVE techniques discussed include the comparison between in vitro and in vivo studies, methods to rene the dose metric or the in vitro models, allometric approach, mechanistic modeling, Multiple-Path Particle Dosimetry (MPPD), methods using organ burden data and also approaches that are currently being developed.

The ubiquitousness of microplastics (<5 mm) has become a pressing environmental concern globally due to the extensive use of plastics. Microplastics have been well-studied in aquatic environments but not well-characterized in soils. Present analytical processes to quantify microplastics accurately in soil samples are quite challenging and require improved and validated analytical steps to eliminate the obscurities and biases. We aimed to develop an effective method for the extraction and quantification of microplastics from soil samples. Different ratios of low-(NaCl) and high-density solutions (ZnCl₂/ NaBr) were tested to determine the most efficient combination for density-dependent separation of microplastics from soil. The combination of low- (1:6) and high-density (1:3) solutions {as weight of soil(g)/volume of density solution(ml)} accounted for 95% recovery of the spiked microplastic particles from soil samples. Likewise, different soil-to-solution ratios of H₂O₂ were tested for the removal of soil organic matter with heating and non-heating steps. Prior removal of organic matter from soil samples achieved a clear supernatant that facilitated 99% recovery of microplastic particles. The validation of individually spiked microplastic particles of small (10-100 μm) and large scale (100-5000 μm) resulted in recovery ranging from 88 to 99%. A validated modified method with prior digestion followed by density-dependent separation was further tested using the field samples with microplastic contamination. The microplastics of different shapes, sizes, colours and polymeric compositions were reported efficiently and well characterized in the field-collected soil samples using this method.