IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2024.100539

Michael J. Burgum , Víctor Alcolea-Rodríguez , Hanna Saarelainen , Raquel Portela , Julián J. Reinosa , José F. Fernández , Verónica I. Dumit , Julia Catalán , Felice C. Simeone , Lara Faccani , Martin J.D. Clift , Stephen J. Evans , Miguel A. Bañares , Shareen H. Doak

Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the in vitro mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o⁻ human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24 h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o⁻ with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox vs. high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized in chemico as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms.

多壁碳纳米管（MWCNTs）由于其广泛的应用而成为一类理想的高纵横比纳米材料（HARNs）。鉴于这些材料的需求，越来越多的职业和消费者接触到这些材料，有必要对它们可能对人类健康造成的潜在危害进行广泛调查。本研究利用体外哺乳动物细胞基因突变和细胞动力学阻断微核（CBMN）试验，研究暴露于NM-400和NM-401 MWCNTs 24 h后，人淋巴母细胞样细胞（TK6）和16HBE14o-人肺上皮细胞的遗传毒性。为了评估继发性遗传毒性的可能性，将CBMN试验应用于16HBE14o-与分化的人单核细胞（dTHP-1）共培养。此外，在暴露前和脱细胞实验中，使用了两种分散方法（NanoGenoTox和高剪切混合）来评估对MWCNT氧化电位、纵横比和表面性质的影响。用化学、电子显微镜和拉曼光谱对它们进行了表征。两种分散方法均观察到NM-400的结构损伤；拉曼光谱强调了探针超声下更大的氧化转变，而不是高剪切混合。尽管MWCNTs的氧化电位发生了变化，但在施加的条件下没有观察到统计学上显著的遗传毒性。在测试条件下，NM-400或NM-401也没有明显的细胞内化迹象，这可能支持负遗传毒性反应。虽然这些harn可能具有氧化潜能，但细胞具有修复瞬时DNA损伤的天然保护机制。因此，评估测量固定DNA损伤的生物终点以解释DNA修复机制的影响是至关重要的。

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引用次数: 0

Nanoplastics in focus: Exploring interdisciplinary approaches and future directions

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100544

Julien Gigault , Mélanie Davranche

Nanoplastics (NPs) are gaining increasing attention due to their widespread distribution and potential environmental and biological impacts. Spanning a variety of ecosystems – from soils and rivers to oceans and polar ice – NPs interact with complex biological and geochemical processes, posing risks to organisms across multiple trophic levels. Despite their growing presence, understanding the behavior, transport, and toxicity of nanoplastics remains challenging due to their diverse physical and chemical properties as well as the heterogeneity of environmental matrices. Currently, nanoplastics are often studied alongside microplastics as a single, homogeneous group, which obscures the nuanced behavior of NPs, particularly in terms of their colloidal properties and interactions within ecosystems. This perspective aims to highlight the critical gaps in nanoplastics research, stressing the importance for field studies and advanced detection/quantification methods to better capture their behavior across environmental interfaces. We advocate for a more integrated approach that account for the dynamic interactions between nanoplastics and surrounding biological, chemical, and physical environments, especially across key ecological gradients. Furthermore, long-term and transgenerational studies are essential to assess the chronic impacts of low-concentration nanoplastics exposure. Innovative and appropriate methodologies are needed to explore NP fate, transport, and toxicity in realistic environmental conditions. By combining advanced experimental tools, field studies, and ecological modeling frameworks, this paper outlines provides a roadmap for advancing our understanding of nanoplastics and their broader ecological impacts, ultimately shaping more effective environmental monitoring and mitigation strategies.

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引用次数: 0

Titanium dioxide nanoparticles - physicochemical characterization and cytotoxic risk

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100543

Filip Kunc , Xiaomei Du , Andre Zborowski , Linda J. Johnston , David C. Kennedy

Titanium dioxide (TiO₂) nanoparticles (NPs) are incorporated into numerous consumer products yet data as to potential adverse health effects remains inconclusive. In this paper we physically characterize 16 nanoforms of TiO₂ from different manufacturers of different size, crystalline structure and surface chemistry. Physical measurements of the particles were performed and compared with those provided by manufacturers revealing several discrepancies. We then examined the biological effects of these particles in cell culture in 3 commonly used cell lines for testing materials. We were unable to validate that anatase particles are more cytotoxic than rutile particles as has been reported, and generally found that the particles produced few effects and no significant production of reactive oxygen species under the conditions used. While some particles do exhibit a dose dependent cytotoxicity that increases over time in some cell lines, the effects were not consistent between cell lines and do not appear to be linked to crystalline structure or any of the specific physical characteristics that were measured including, size, charge and surface composition, nor a correlation with the production of reactive oxygen species.

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引用次数: 0

Effect of carbon chain length and concentration of perfluorinated compounds on polytetrafluoroethylene microplastics transport behavior

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100550

Shihao Zhao , Ruihao Xu , Xiangying Liu , Yifan Wang , Yanji Jiang

Perfluorooctanoic acid (PFOA) and perfluoropentanoic acid (PFPeA), as important components of perfluorinated compounds (PFAS), are not only ecologically hazardous, but also have surfactant properties that can alter the transport behavior of polytetrafluoroethylene (PTFE) in porous media. In this experiment, the effect of PFAS on the transport of PTFE in porous media under different pH, ionic strength (IS) and ion valence states was studied. The results showed that the recovery rate of PTFE decreased gradually with the decrease of pH and the increase of IS and ion valence states. When the above conditions change, the double electron layer on the microplastic surface is compressed, the absolute value of zeta potential decreases, the repulsion between each other decreases, and aggregation and deposition are more likely. In addition, it was found that the recovery rate of PTFE co-transported with long chain PFOA was higher than that of short chain PFPeA. This phenomenon may be caused by the adhesion ability of PFOA with long carbon chain on the surface of PTFE is greater than that of PFPeA with short carbon chain. On the other hand, PFAS with different carbon chain lengths produce different spatial site resistance effects after binding with particles, and the spatial site resistance produced by the long-chain PFOA is larger than that of the short-chain PFPeA, leading to a decrease in particle-to-particle aggregation and a better transport effect. This study will help to understand the effects of PFAS with different carbon chain lengths on the transport of microplastics in porous media, as well as the transport rule of PTFE under different conditions, and provide reference value for the calculation of its flux in soil.

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引用次数: 0

Impacts of polyglycolic acid and analogues on glycolipid metabolism and circadian behavior in zebrafish

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100546

Liang Wen , Shuhui Zhang , Jialu Luan , Tian Yin , Xizeng Feng

For the past few years, new biodegradable polymers, such as polyglycolic acid (PGA) and polylactic acid (PLA), have been promising materials to solve the remarkable environmental issue, of microplastics (MPs) pollution. In this research, the impacts of five MPs, including PGA, PLA, polybutylene succinate (PBS), polyhydroxyalkanoate (PHA), and polybutylene adipate terephthalate (PBAT), were analyzed on zebrafish with different concentrations. We found that PGA and PLA at 1 mg/L did not have obvious effects on liver function, glucose level, and circadian rhythm in larvae. However, Exposure to PBS, PHA, and PBAT at 1 mg/L could cause mild pathological injury of the liver and decreased glucose levels. Furthermore, exposure to PBS, PHA, and PBAT at 100 mg/L caused abnormal early development and pathological injury of the liver, increased ALT and TG levels, as well as decreased glucose levels. The molecular explanation of this was the variational expression levels of genes related to many aspects of biochemical pathways, such as oxidative stress, apoptosis, endoplasmic reticulum stress, fatty acid oxidation, and glucose metabolism. Meanwhile, larvae exposed to PBS, PHA, and PBAT at 100 mg/L showed chaos in circadian behaviors, accompanied by the disturbed expression of clock genes. Overall, we observed a greater adverse effect of PBS, PHA, and PBAT relative to PLA and PGA when we compared the effects induced by five MPs at the same exposure concentration. Our study provided important data to evaluate the ecological risk of new biodegradable polymers.

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引用次数: 0

Predicting the dissolution of metal-based nanoparticles by means of QSPRs and the effect of data augmentation

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100547

Yuchao Song , Surendra Balraadjsing , Willie J.G.M. Peijnenburg , Martina G. Vijver

Particle dissolution is a critical process in the environmental fate assessment of metal-based nanoparticles (MNPs). Numerous attempts have been made previously to adequately quantify dissolution (kinetics), however, existing dissolution data and models are generally limited to a few nanomaterials or specific time points. Hence, they only capture phases of the process. This study aimed to develop a Quantitative Structure-Property Relationship (QSPR) model to predict the ion release (in %) of MNPs for different time points and water chemistry conditions. Furthermore, many machine learning models are frequently plagued by a lack of data and recently data augmentation has been suggested as a method to mitigate this issue. Therefore, we also investigated the effects of data augmentation on QSPRs. Following data collection from literature, QSPR models were generated and results indicate models with adequate performance (R² > 0.7). Results also demonstrated significant improvements in model performance with increasing amounts of applied data augmentation. However, a deeper evaluation of the results also highlighted that data augmentation can lead to misleading and overoptimistic model evaluation. Thus, proper model assessment is necessary when evaluating QSPRs. Variable importance analysis results revealed that the “initial concentration” and features related to the size and shape of MNPs were the most critical factors in the dissolution process. The predictive models generated here for MNP dissolution can improve nanomaterial testing efficiency and guide experimental design.

{"title":"Predicting the dissolution of metal-based nanoparticles by means of QSPRs and the effect of data augmentation","authors":"Yuchao Song , Surendra Balraadjsing , Willie J.G.M. Peijnenburg , Martina G. Vijver","doi":"10.1016/j.impact.2025.100547","DOIUrl":"10.1016/j.impact.2025.100547","url":null,"abstract":"<div><div>Particle dissolution is a critical process in the environmental fate assessment of metal-based nanoparticles (MNPs). Numerous attempts have been made previously to adequately quantify dissolution (kinetics), however, existing dissolution data and models are generally limited to a few nanomaterials or specific time points. Hence, they only capture phases of the process. This study aimed to develop a Quantitative Structure-Property Relationship (QSPR) model to predict the ion release (in %) of MNPs for different time points and water chemistry conditions. Furthermore, many machine learning models are frequently plagued by a lack of data and recently data augmentation has been suggested as a method to mitigate this issue. Therefore, we also investigated the effects of data augmentation on QSPRs. Following data collection from literature, QSPR models were generated and results indicate models with adequate performance (R<sup>2</sup> > 0.7). Results also demonstrated significant improvements in model performance with increasing amounts of applied data augmentation. However, a deeper evaluation of the results also highlighted that data augmentation can lead to misleading and overoptimistic model evaluation. Thus, proper model assessment is necessary when evaluating QSPRs. Variable importance analysis results revealed that the “initial concentration” and features related to the size and shape of MNPs were the most critical factors in the dissolution process. The predictive models generated here for MNP dissolution can improve nanomaterial testing efficiency and guide experimental design.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"37 ","pages":"Article 100547"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-sized polystyrene plastics toxicity: Necroptosis pathway caused by autophagy blockade and lysosomal dysfunction 纳米聚苯乙烯塑料毒性：自噬阻断和溶酶体功能障碍引起的坏死坏死途径。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2024.100537

Haiyan Wu , Runqiu Cai , Chaoyu Zhou , Yifei Yang , Xinyuan Tian , Zhongling Zhao , Qianyu Bai , Xuejiao Qiu , Qingyi Song , Lei Zhang , Huihui Bao , Tianlong Liu

The persistent detection of nano-sized plastic particles in humans, animals, and animal-derived products underscores the potential impact of these particles on living organisms. Consequently, the toxicology of such particles has emerged as a pivotal research interests in recent years. In this study, NP was synthesized successfully with an average particle size of 100 nm using a emulsion polymerization method as model particles. Following co-incubation of IEC-6 cells with NP for 24–168 h, a notable inhibition of cell viability and proliferation was observed. The significant activation of autophagy and a concomitant blockage of autophagic flux in IEC-6 cells after 24–72 h of co-incubation with NP were unveiled by transmission electron microscopy, western blotting, and double-fluorescent autophagy analysis. A significant increase in the number of lysosomes and an increase in the expression of hydrolase CTSB were detected, indicating dysregulation of lysosomal function. The subsequent transcriptomic and metabolomics analyses, coupled with the observation of activated lysosomes and the RIPK1-RIPK3-MLKL/PYGL pathway, led us to posit that the blockade of autophagy and lysosomal dysfunction, culminating in lysosomal membrane permeabilization (LMP) induced necroptosis, constitutes one of the mechanisms contributing to the cytotoxicity of NP.

Synopsis

The cytotoxicity and its related mechanisms of nano-plastic is still unclear. This study found that nano-plastics may induce necroptosis in cells, and autophagy blockade and lysosomal dysfunction are prodromal manifestations.

在人类、动物和动物衍生产品中持续检测到纳米级塑料颗粒，强调了这些颗粒对生物体的潜在影响。因此，近年来，这些颗粒的毒理学已成为一个关键的研究兴趣。本研究以乳液聚合法为模型粒子成功合成了平均粒径为100 nm的NP。IEC-6细胞与NP共孵育24-168 h后，观察到细胞活力和增殖受到明显抑制。透射电镜、western blotting和双荧光自噬分析显示，与NP共孵育24-72 h后，IEC-6细胞的自噬明显激活，自噬通量阻滞。检测到溶酶体数量显著增加，水解酶CTSB表达增加，表明溶酶体功能失调。随后的转录组学和代谢组学分析，加上对活化溶酶体和RIPK1-RIPK3-MLKL/PYGL通路的观察，使我们假设自噬和溶酶体功能障碍的阻断，最终导致溶酶体膜透性（LMP）诱导的坏死坏死，是促成NP细胞毒性的机制之一。摘要：纳米塑料的细胞毒性及其相关机制尚不清楚。本研究发现纳米塑料可诱导细胞坏死，自噬阻断和溶酶体功能障碍是前驱症状。

{"title":"Nano-sized polystyrene plastics toxicity: Necroptosis pathway caused by autophagy blockade and lysosomal dysfunction","authors":"Haiyan Wu , Runqiu Cai , Chaoyu Zhou , Yifei Yang , Xinyuan Tian , Zhongling Zhao , Qianyu Bai , Xuejiao Qiu , Qingyi Song , Lei Zhang , Huihui Bao , Tianlong Liu","doi":"10.1016/j.impact.2024.100537","DOIUrl":"10.1016/j.impact.2024.100537","url":null,"abstract":"<div><div>The persistent detection of nano-sized plastic particles in humans, animals, and animal-derived products underscores the potential impact of these particles on living organisms. Consequently, the toxicology of such particles has emerged as a pivotal research interests in recent years. In this study, NP was synthesized successfully with an average particle size of 100 nm using a emulsion polymerization method as model particles. Following co-incubation of IEC-6 cells with NP for 24–168 h, a notable inhibition of cell viability and proliferation was observed. The significant activation of autophagy and a concomitant blockage of autophagic flux in IEC-6 cells after 24–72 h of co-incubation with NP were unveiled by transmission electron microscopy, western blotting, and double-fluorescent autophagy analysis. A significant increase in the number of lysosomes and an increase in the expression of hydrolase CTSB were detected, indicating dysregulation of lysosomal function. The subsequent transcriptomic and metabolomics analyses, coupled with the observation of activated lysosomes and the RIPK1-RIPK3-MLKL/PYGL pathway, led us to posit that the blockade of autophagy and lysosomal dysfunction, culminating in lysosomal membrane permeabilization (LMP) induced necroptosis, constitutes one of the mechanisms contributing to the cytotoxicity of NP.</div></div><div><h3>Synopsis</h3><div>The cytotoxicity and its related mechanisms of nano-plastic is still unclear. This study found that nano-plastics may induce necroptosis in cells, and autophagy blockade and lysosomal dysfunction are prodromal manifestations.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"37 ","pages":"Article 100537"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The risk of short-term microplastic exposure on female reproductive function: A rat model study

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100545

Zihan Wang , Ruiqing Zhang , Yuanzhen Zhang , Yao Xiong , Ming Zhang

Long-term effects of microplastics (MPs) exposure have been demonstrated to impair reproductive function. However, in real world, the exposure level of MP is not constant and it may vary in different individuals. This study aims to evaluate the impact of short-term exposure to MPs on ovarian and endometrial function in rat models. Serum steroid hormone concentrations and the expression of ovarian steroid hormone receptor were disturbed. We found that as MPs exposure concentration increased, thickness of the endometrial glandular epithelial layer and the number of endometrial glands decreased; the number of primordial follicles decreased, while the numbers of primary and secondary oocytes significantly increased, indicating a potential oocyte overactivation. Although short-term MP exposure appears to not influence embryo implantation and hormone functions, the results of this study highlight the potential of MPs to disrupt reproductive health in women.

引用次数: 0

Gastrointestinal dysfunction as the main performance of the oral toxicity of titanium dioxide nanoparticle on gastric ulcer rats 胃肠功能紊乱是纳米二氧化钛颗粒对胃溃疡大鼠口服毒性的主要表现。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100551

Yun Wang , Chen Li , Te Ba , Shengyuan Wang , Langzhi He , Zhangjian Chen , Ji Pu , Xiaoxing Cui , Guang Jia

Titanium dioxide nanoparticles (TiO₂ NPs) have promising applications in food additives and pharmaceutical dressings, raising concerns about their oral safety. The current studies mainly focus on healthy groups, and the effect of TiO₂ NPs on the patient population is rarely known. Here, a comprehensive toxicity study of TiO₂ NPs (75 ± 15 nm, anatase) in gastric ulcer rats (male 8-week old Sprague-Dawley rats) is reported following oral exposure at dose of 0, 10, 50, 200 mg/kg body weight per day for 30 days. The gastric ulcer rats were produced by submucosal injection of acetic acid solution into the rat stomach. The healthy rats were used as the normal control. We evaluated nanoparticle biodistribution, systemic toxicity, and gastrointestinal function indices in the rats. Our findings indicate that oral administration of TiO₂ NPs resulted in minimal intestinal absorption and transport with limited systemic organ toxicity. The internalization of TiO₂ NPs and activation of mast cells in the stomach tissues, along with the low serum levels of histamine and IgE, suggest a localized allergic reaction rather than a systemic one. Furthermore, the notably reduced plasma levels of D-lactate and the activity of diamine oxidase (DAO) indicated the upregulation of intestinal barrier function. These statistically significant results indicated that gastrointestinal dysfunction was the main performance of the oral toxicity of TiO₂ NPs on gastric ulcer rats, emphasizing the importance of controlling the intake of TiO₂ NPs in patients with gastric ulcers.

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引用次数: 0

Absolute quantitative lipidomics reveals the disturbance of lipid metabolism induced by oral exposure of titanium dioxide nanoparticles 绝对定量脂质组学揭示了口服二氧化钛纳米颗粒引起的脂质代谢紊乱。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100554

Nairui Yu , Jiaqi Shi , Ying Ma , Yi Zhang , Li Guan , Zhangjian Chen , Guang Jia

The widespread use of titanium dioxide nanoparticles (TiO₂ NPs) as a white pigment in consumer goods increases the possibility of its release into the environment, which poses a great health risk to human beings. Many studies have proved the liver damage caused by TiO₂ NPs, but the research about the potential effects of TiO₂ NPs on liver lipid metabolism has been limited. Therefore, we selected Sprague-Dawley (SD) rats to explore the effects of long-term exposure to TiO₂ NPs on lipid metabolism. Rats were exposed to TiO₂ NPs at 0, 2, 10, 50 mg/kg body weight daily for 90 consecutive days. Subsequently, absolute quantitative lipidomics was used to ascertain variation of differential lipid metabolites in rat liver and serum. The results showed that TiO₂ NPs (50 mg/kg) changed 22 lipid metabolites such as DAG (18:2/20:5) and TAG (58:10/FA18:2) in rat liver. In the serum, the alteration of 119 lipid metabolites such as DAG (18:0/18:2) were more significant. There was a significant correlation between the different lipid metabolites in liver and serum. At the same time, it was observed that the relative expression levels of oxidative stress-related genes Nrf-2 and Ho-1 changed significantly, and they were closely related to differential metabolites. In conclusion, oral exposure of TiO₂ NPs has changed the lipid metabolomics of liver and serum, and the strong induction of oxidative stress may be related to it. TAG and DAG are key metabolites and metabolic pathways in two distinct biological samples, serving as potential indicators of liver injury to a certain extent.

{"title":"Absolute quantitative lipidomics reveals the disturbance of lipid metabolism induced by oral exposure of titanium dioxide nanoparticles","authors":"Nairui Yu , Jiaqi Shi , Ying Ma , Yi Zhang , Li Guan , Zhangjian Chen , Guang Jia","doi":"10.1016/j.impact.2025.100554","DOIUrl":"10.1016/j.impact.2025.100554","url":null,"abstract":"<div><div>The widespread use of titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) as a white pigment in consumer goods increases the possibility of its release into the environment, which poses a great health risk to human beings. Many studies have proved the liver damage caused by TiO<sub>2</sub> NPs, but the research about the potential effects of TiO<sub>2</sub> NPs on liver lipid metabolism has been limited. Therefore, we selected Sprague-Dawley (SD) rats to explore the effects of long-term exposure to TiO<sub>2</sub> NPs on lipid metabolism. Rats were exposed to TiO<sub>2</sub> NPs at 0, 2, 10, 50 mg/kg body weight daily for 90 consecutive days. Subsequently, absolute quantitative lipidomics was used to ascertain variation of differential lipid metabolites in rat liver and serum. The results showed that TiO<sub>2</sub> NPs (50 mg/kg) changed 22 lipid metabolites such as DAG (18:2/20:5) and TAG (58:10/FA18:2) in rat liver. In the serum, the alteration of 119 lipid metabolites such as DAG (18:0/18:2) were more significant. There was a significant correlation between the different lipid metabolites in liver and serum. At the same time, it was observed that the relative expression levels of oxidative stress-related genes <em>Nrf-2</em> and <em>Ho-1</em> changed significantly, and they were closely related to differential metabolites. In conclusion, oral exposure of TiO<sub>2</sub> NPs has changed the lipid metabolomics of liver and serum, and the strong induction of oxidative stress may be related to it. TAG and DAG are key metabolites and metabolic pathways in two distinct biological samples, serving as potential indicators of liver injury to a certain extent.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"37 ","pages":"Article 100554"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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