新型持久性植物 RNA 病毒携带突变,削弱了病毒对抗病毒 RNA 干扰的抑制作用。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-10-01 DOI:10.1111/mpp.70020
Li-Juan Zhu, Yu Zhu, Chengwu Zou, Lan-Yi Su, Chong-Tao Zhang, Chi Wang, Ya-Ni Bai, Baoshan Chen, Rongbai Li, Qingfa Wu, Shou-Wei Ding, Jian-Guo Wu, Yan-Hong Han
{"title":"新型持久性植物 RNA 病毒携带突变,削弱了病毒对抗病毒 RNA 干扰的抑制作用。","authors":"Li-Juan Zhu, Yu Zhu, Chengwu Zou, Lan-Yi Su, Chong-Tao Zhang, Chi Wang, Ya-Ni Bai, Baoshan Chen, Rongbai Li, Qingfa Wu, Shou-Wei Ding, Jian-Guo Wu, Yan-Hong Han","doi":"10.1111/mpp.70020","DOIUrl":null,"url":null,"abstract":"<p><p>Persistent plant viruses are widespread in natural ecosystems. However, little is known about why persistent infection with these viruses may cause little or no harm to their host. Here, we discovered a new polerovirus that persistently infected wild rice plants by deep sequencing and assembly of virus-derived small-interfering RNAs (siRNAs). The new virus was named Rice tiller inhibition virus 2 (RTIV2) based on the symptoms developed in cultivated rice varieties following Agrobacterium-mediated inoculation with an infectious RTIV2 clone. We showed that RTIV2 infection induced antiviral RNA interference (RNAi) in both the wild and cultivated rice plants as well as Nicotiana benthamiana. It is known that virulent virus infection in plants depends on effective suppression of antiviral RNAi by viral suppressors of RNAi (VSRs). Notably, the P0 protein of RTIV2 exhibited weak VSR activity and carries alanine substitutions of two amino acids broadly conserved among diverse poleroviruses. Mixed infection with umbraviruses enhanced RTIV2 accumulation and/or enabled its mechanical transmission in N. benthamiana. Moreover, replacing the alanine at either one or both positions of RTIV2 P0 enhanced the VSR activity in a co-infiltration assay, and RTIV2 mutants carrying the corresponding substitutions replicated to significantly higher levels in both rice and N. benthamiana plants. Together, our findings show that as a persistent plant virus, RTIV2 carries specific mutations in its VSR gene to weaken viral suppression of antiviral RNAi. Our work reveals a new strategy for persistent viruses to maintain long-term infection by weak suppression of the host defence response.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513406/pdf/","citationCount":"0","resultStr":"{\"title\":\"New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.\",\"authors\":\"Li-Juan Zhu, Yu Zhu, Chengwu Zou, Lan-Yi Su, Chong-Tao Zhang, Chi Wang, Ya-Ni Bai, Baoshan Chen, Rongbai Li, Qingfa Wu, Shou-Wei Ding, Jian-Guo Wu, Yan-Hong Han\",\"doi\":\"10.1111/mpp.70020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Persistent plant viruses are widespread in natural ecosystems. However, little is known about why persistent infection with these viruses may cause little or no harm to their host. Here, we discovered a new polerovirus that persistently infected wild rice plants by deep sequencing and assembly of virus-derived small-interfering RNAs (siRNAs). The new virus was named Rice tiller inhibition virus 2 (RTIV2) based on the symptoms developed in cultivated rice varieties following Agrobacterium-mediated inoculation with an infectious RTIV2 clone. We showed that RTIV2 infection induced antiviral RNA interference (RNAi) in both the wild and cultivated rice plants as well as Nicotiana benthamiana. It is known that virulent virus infection in plants depends on effective suppression of antiviral RNAi by viral suppressors of RNAi (VSRs). Notably, the P0 protein of RTIV2 exhibited weak VSR activity and carries alanine substitutions of two amino acids broadly conserved among diverse poleroviruses. Mixed infection with umbraviruses enhanced RTIV2 accumulation and/or enabled its mechanical transmission in N. benthamiana. Moreover, replacing the alanine at either one or both positions of RTIV2 P0 enhanced the VSR activity in a co-infiltration assay, and RTIV2 mutants carrying the corresponding substitutions replicated to significantly higher levels in both rice and N. benthamiana plants. Together, our findings show that as a persistent plant virus, RTIV2 carries specific mutations in its VSR gene to weaken viral suppression of antiviral RNAi. Our work reveals a new strategy for persistent viruses to maintain long-term infection by weak suppression of the host defence response.</p>\",\"PeriodicalId\":18763,\"journal\":{\"name\":\"Molecular plant pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513406/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular plant pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/mpp.70020\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.70020","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

持久性植物病毒广泛存在于自然生态系统中。然而,人们对这些病毒的持续感染为何会对宿主造成很少或根本不造成伤害却知之甚少。在这里,我们通过对病毒衍生的小干扰 RNA(siRNA)进行深度测序和组装,发现了一种能持续感染野生水稻植株的新型多核病毒。这种新病毒被命名为水稻分蘖抑制病毒 2(Rice tiller inhibition virus 2,RTIV2),这是基于农杆菌介导接种具有感染性的 RTIV2 克隆后,栽培稻品种出现的症状。我们的研究表明,RTIV2 感染可诱导野生水稻、栽培水稻和烟草植物的抗病毒 RNA 干扰(RNAi)。众所周知,病毒在植物中的毒性感染取决于病毒 RNAi 抑制剂(VSR)对抗病毒 RNAi 的有效抑制。值得注意的是,RTIV2 的 P0 蛋白具有较弱的 VSR 活性,并带有在多种多角体病毒中广泛保守的两个氨基酸的丙氨酸替代。与伞形病毒混合感染可增强 RTIV2 的积累和/或使其在 N. benthamiana 中进行机械传播。此外,替换 RTIV2 P0 的一个或两个位置上的丙氨酸可增强共渗试验中的 VSR 活性,携带相应替换的 RTIV2 突变体在水稻和 N. benthamiana 植物中的复制水平显著提高。总之,我们的研究结果表明,作为一种持久性植物病毒,RTIV2 的 VSR 基因携带特异性突变,从而削弱了病毒对抗病毒 RNAi 的抑制作用。我们的研究揭示了持久性病毒通过弱化对宿主防御反应的抑制来维持长期感染的新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.

Persistent plant viruses are widespread in natural ecosystems. However, little is known about why persistent infection with these viruses may cause little or no harm to their host. Here, we discovered a new polerovirus that persistently infected wild rice plants by deep sequencing and assembly of virus-derived small-interfering RNAs (siRNAs). The new virus was named Rice tiller inhibition virus 2 (RTIV2) based on the symptoms developed in cultivated rice varieties following Agrobacterium-mediated inoculation with an infectious RTIV2 clone. We showed that RTIV2 infection induced antiviral RNA interference (RNAi) in both the wild and cultivated rice plants as well as Nicotiana benthamiana. It is known that virulent virus infection in plants depends on effective suppression of antiviral RNAi by viral suppressors of RNAi (VSRs). Notably, the P0 protein of RTIV2 exhibited weak VSR activity and carries alanine substitutions of two amino acids broadly conserved among diverse poleroviruses. Mixed infection with umbraviruses enhanced RTIV2 accumulation and/or enabled its mechanical transmission in N. benthamiana. Moreover, replacing the alanine at either one or both positions of RTIV2 P0 enhanced the VSR activity in a co-infiltration assay, and RTIV2 mutants carrying the corresponding substitutions replicated to significantly higher levels in both rice and N. benthamiana plants. Together, our findings show that as a persistent plant virus, RTIV2 carries specific mutations in its VSR gene to weaken viral suppression of antiviral RNAi. Our work reveals a new strategy for persistent viruses to maintain long-term infection by weak suppression of the host defence response.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
期刊最新文献
Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis. The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility. A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance. Flg22-facilitated PGPR colonization in root tips and control of root rot. A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Nytbr.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1