关于几分钟的生物分析：用于尿液 2-苯基-2-丙醇监测的自动冷却-SPME 和快速-GC。

IF 3.9 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Toxics Pub Date : 2024-10-13 DOI:10.3390/toxics12100743

Stefano Dugheri, Niccolò Fanfani, Giovanni Cappelli, Antonio Marigliano, Elisabetta Bucaletti, Donato Squillaci, Ilaria Rapi, Lorenzo Venturini, Giulia Pizzella, Sara Manetta, Alfonso Pavone, Michele Secchi, Iacopo Rainaldi, Nicola Mucci

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引用次数: 0

摘要

本研究开发了一种创新的 SPME 顶空 GC-MS 方法，该方法采用冷却模式和全自动例行程序，用于检测 2-苯基-2-丙醇（一种具有代表性的尿液中的积苯代谢物）。在使用较低量的试剂（醋酸酐和吡啶）进行酸水解和衍生化步骤后，使用 30 μm 聚二甲基硅氧烷 SPME 纤维从顶空采样衍生化的 2-苯基-2-丙醇，如苯甲醇、α、α-二甲基乙酸酯。通过实验设计优化后，该方法的检出限（LOD）为 0.034 mg/L，定量限（LOQ）为 0.10 mg/L，每个样品所需的采样时间很短。该方法是在标准溶液的基础上开发的，将适用于职业暴露和非职业暴露人群。

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Regarding Bioanalysis Lasting a Few Minutes: Automated Cooling-SPME and Fast-GC for Urinary 2-Phenyl-2-Propanol Monitoring.

An innovative SPME head space GC-MS method, in cooling mode, using a fully automated routine, was developed to detect 2-phenyl-2-propanol, a representative urinary metabolite of cumene. Following an acid hydrolysis and derivatization step with lowered quantities of reagents, acetic anhydride and pyridine, a 30 μm polydimethylsiloxane SPME fiber was used to sample derivatized 2-phenyl-2-propanol, such as benzenemethanol,α,α-dimethyl-acetate, from the headspace. Performances of the method, optimized through experimental design, provide an LOD of 0.034 mg/L and an LOQ 0.10 mg/L, with a short sampling time necessary per sample. The method, developed on standard solutions, will be applied to both occupationally exposed and non-exposed populations.

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来源期刊

Toxics Chemical Engineering-Chemical Health and Safety

CiteScore

4.50

自引率

10.90%

发文量

681

审稿时长

6 weeks

期刊介绍： The Journal accepts papers describing work that furthers our understanding of the exposure, effects, and risks of chemicals and materials in humans and the natural environment as well as approaches to assess and/or manage the toxicological and ecotoxicological risks of chemicals and materials. The journal covers a wide range of toxic substances, including metals, pesticides, pharmaceuticals, biocides, nanomaterials, and polymers such as micro- and mesoplastics. Toxics accepts papers covering: The occurrence, transport, and fate of chemicals and materials in different systems (e.g., food, air, water, soil); Exposure of humans and the environment to toxic chemicals and materials as well as modelling and experimental approaches for characterizing the exposure in, e.g., water, air, soil, food, and consumer products; Uptake, metabolism, and effects of chemicals and materials in a wide range of systems including in-vitro toxicological assays, aquatic and terrestrial organisms and ecosystems, model mammalian systems, and humans; Approaches to assess the risks of chemicals and materials to humans and the environment; Methodologies to eliminate or reduce the exposure of humans and the environment to toxic chemicals and materials.