嗜热四膜虫的金属硫蛋白系统是铁诱导型的

IF 3.9 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Toxics Pub Date : 2024-10-08 DOI:10.3390/toxics12100725
Davide Gualandris, Davide Rotondo, Candida Lorusso, Antonietta La Terza, Antonio Calisi, Francesco Dondero
{"title":"嗜热四膜虫的金属硫蛋白系统是铁诱导型的","authors":"Davide Gualandris, Davide Rotondo, Candida Lorusso, Antonietta La Terza, Antonio Calisi, Francesco Dondero","doi":"10.3390/toxics12100725","DOIUrl":null,"url":null,"abstract":"<p><p>Metallothioneins are multifunctional proteins implicated in various cellular processes. They have been used as biomarkers of heavy metal exposure and contamination due to their intrinsic ability to bind heavy metals and their transcriptional response to both physiological and noxious metal ions such as cadmium (Cd) and mercury (Hg). In this study, we aimed to clarify the role of iron and reactive oxygen species (ROSs) in the induction of the metallothionein system (Mtt) in the ciliate protozoan <i>Tetrahymena thermophila</i>. We investigated the relative mRNA abundances of the metallothionein genes Mtt1, Mtt2/4, and Mtt5, revealing for the first time their responsiveness to iron exposure. Furthermore, by using inhibitors of superoxide dismutase (SOD) and catalase (CAT), alone or in combination with iron, we highlighted the roles of superoxide ion and endogenous hydrogen peroxide, as well as the complex interplay between the metal and ROSs. These results enhance our understanding of the metallothionein system in ciliates and suggest that ROSs may be a primary evolutionary driver for the selection of these proteins in nature.</p>","PeriodicalId":23195,"journal":{"name":"Toxics","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511230/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Metallothionein System in <i>Tetrahymena thermophila</i> Is Iron-Inducible.\",\"authors\":\"Davide Gualandris, Davide Rotondo, Candida Lorusso, Antonietta La Terza, Antonio Calisi, Francesco Dondero\",\"doi\":\"10.3390/toxics12100725\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Metallothioneins are multifunctional proteins implicated in various cellular processes. They have been used as biomarkers of heavy metal exposure and contamination due to their intrinsic ability to bind heavy metals and their transcriptional response to both physiological and noxious metal ions such as cadmium (Cd) and mercury (Hg). In this study, we aimed to clarify the role of iron and reactive oxygen species (ROSs) in the induction of the metallothionein system (Mtt) in the ciliate protozoan <i>Tetrahymena thermophila</i>. We investigated the relative mRNA abundances of the metallothionein genes Mtt1, Mtt2/4, and Mtt5, revealing for the first time their responsiveness to iron exposure. Furthermore, by using inhibitors of superoxide dismutase (SOD) and catalase (CAT), alone or in combination with iron, we highlighted the roles of superoxide ion and endogenous hydrogen peroxide, as well as the complex interplay between the metal and ROSs. These results enhance our understanding of the metallothionein system in ciliates and suggest that ROSs may be a primary evolutionary driver for the selection of these proteins in nature.</p>\",\"PeriodicalId\":23195,\"journal\":{\"name\":\"Toxics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511230/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxics\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.3390/toxics12100725\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxics","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.3390/toxics12100725","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

金属硫蛋白是一种参与多种细胞过程的多功能蛋白质。由于金属硫蛋白具有与重金属结合的内在能力以及对生理和有害金属离子(如镉(Cd)和汞(Hg))的转录反应,它们已被用作重金属暴露和污染的生物标志物。本研究旨在阐明铁和活性氧(ROS)在纤毛虫原生动物嗜热四膜虫金属硫蛋白系统(Mtt)诱导过程中的作用。我们研究了金属硫蛋白基因 Mtt1、Mtt2/4 和 Mtt5 的相对 mRNA 丰度,首次揭示了它们对铁暴露的反应性。此外,通过使用超氧化物歧化酶(SOD)和过氧化氢酶(CAT)抑制剂(单独或与铁结合使用),我们强调了超氧离子和内源性过氧化氢的作用,以及金属和 ROS 之间复杂的相互作用。这些结果加深了我们对纤毛虫金属硫蛋白系统的了解,并表明 ROS 可能是自然界选择这些蛋白质的主要进化驱动力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The Metallothionein System in Tetrahymena thermophila Is Iron-Inducible.

Metallothioneins are multifunctional proteins implicated in various cellular processes. They have been used as biomarkers of heavy metal exposure and contamination due to their intrinsic ability to bind heavy metals and their transcriptional response to both physiological and noxious metal ions such as cadmium (Cd) and mercury (Hg). In this study, we aimed to clarify the role of iron and reactive oxygen species (ROSs) in the induction of the metallothionein system (Mtt) in the ciliate protozoan Tetrahymena thermophila. We investigated the relative mRNA abundances of the metallothionein genes Mtt1, Mtt2/4, and Mtt5, revealing for the first time their responsiveness to iron exposure. Furthermore, by using inhibitors of superoxide dismutase (SOD) and catalase (CAT), alone or in combination with iron, we highlighted the roles of superoxide ion and endogenous hydrogen peroxide, as well as the complex interplay between the metal and ROSs. These results enhance our understanding of the metallothionein system in ciliates and suggest that ROSs may be a primary evolutionary driver for the selection of these proteins in nature.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Toxics
Toxics Chemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍: The Journal accepts papers describing work that furthers our understanding of the exposure, effects, and risks of chemicals and materials in humans and the natural environment as well as approaches to assess and/or manage the toxicological and ecotoxicological risks of chemicals and materials. The journal covers a wide range of toxic substances, including metals, pesticides, pharmaceuticals, biocides, nanomaterials, and polymers such as micro- and mesoplastics. Toxics accepts papers covering: The occurrence, transport, and fate of chemicals and materials in different systems (e.g., food, air, water, soil); Exposure of humans and the environment to toxic chemicals and materials as well as modelling and experimental approaches for characterizing the exposure in, e.g., water, air, soil, food, and consumer products; Uptake, metabolism, and effects of chemicals and materials in a wide range of systems including in-vitro toxicological assays, aquatic and terrestrial organisms and ecosystems, model mammalian systems, and humans; Approaches to assess the risks of chemicals and materials to humans and the environment; Methodologies to eliminate or reduce the exposure of humans and the environment to toxic chemicals and materials.
期刊最新文献
Relationship Between Perfluoroalkyl Acids in Human Serum and Sjogren's Syndrome: A Case-Control Study of Populations in Hangzhou, China. Micro(nano)plastic and Related Chemicals: Emerging Contaminants in Environment, Food and Health Impacts. The Physiological Response of Salix matsudana for Water Pollution by 2,4-Dinitrophenol. Effects of Ciprofloxacin on the Production and Composition of Cellular Microcystins in Microcystis aeruginosa. Effect of Electrode Positioning on Electrokinetic Remediation of Contaminated Soft Clay with Surface Electrolyte.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1