猫科葡萄球菌 C4 在新型犬皮肤外植体模型中对耐甲氧西林伪中间葡萄球菌具有体外抗菌活性。

IF 1.9 3区 农林科学 Q3 DERMATOLOGY Veterinary dermatology Pub Date : 2024-10-25 DOI:10.1111/vde.13308
Mayane Faccin, Alan M O'Neill, Sara D Lawhon, Kate A Worthing, Dominique J Wiener, Richard L Gallo, Aline Rodrigues Hoffmann
{"title":"猫科葡萄球菌 C4 在新型犬皮肤外植体模型中对耐甲氧西林伪中间葡萄球菌具有体外抗菌活性。","authors":"Mayane Faccin, Alan M O'Neill, Sara D Lawhon, Kate A Worthing, Dominique J Wiener, Richard L Gallo, Aline Rodrigues Hoffmann","doi":"10.1111/vde.13308","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Canine superficial pyoderma is a common bacterial skin infection of dogs, generally caused by Staphylococcus pseudintermedius. The C4 strain of Staphylococcus felis was recently discovered to have strong antimicrobial activity against S. pseudintermedius in mice.</p><p><strong>Objectives: </strong>We aimed to evaluate in vitro if this antimicrobial activity was maintained using a novel canine skin explant model.</p><p><strong>Materials and methods: </strong>Punch biopsies (8 mm) of skin from recently euthanised dogs were collected and placed into six-well plates on top of an agarose pedestal.</p><p><strong>Results: </strong>Histological examination of the skin explants showed an intact dermal-epidermal organisation and a stratum corneum that was successfully colonised by S. pseudintermedius after topical application. The number of colony forming units of S. pseudintermedius showed a 2 log increase after 24 h colonisation, indicating that the explant supported bacterial growth. By contrast, co-treatment with S. felis C4 live bacteria and its sterile protein product significantly reduced the growth of a methicillin-susceptible (ST540, p = 0.0357) and a methicillin-resistant (MR) strain (ST71, p = 0.0143) of S. pseudintermedius. No detectable bacteria were recovered from or visualised on skin 24 h posttreatment with the S. felis C4 sterile protein product.</p><p><strong>Conclusions and clinical relevance: </strong>Using a novel canine explant model, we demonstrate that the S. felis C4 strain inhibits the growth of S. pseudintermedius and that it is a promising candidate for a new probiotic therapy to treat cutaneous infections caused by S. pseudintermedius, including MR strains.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Staphylococcus felis C4 exhibits in vitro antimicrobial activity against methicillin-resistant Staphylococcus pseudintermedius in a novel canine skin explant model.\",\"authors\":\"Mayane Faccin, Alan M O'Neill, Sara D Lawhon, Kate A Worthing, Dominique J Wiener, Richard L Gallo, Aline Rodrigues Hoffmann\",\"doi\":\"10.1111/vde.13308\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Canine superficial pyoderma is a common bacterial skin infection of dogs, generally caused by Staphylococcus pseudintermedius. The C4 strain of Staphylococcus felis was recently discovered to have strong antimicrobial activity against S. pseudintermedius in mice.</p><p><strong>Objectives: </strong>We aimed to evaluate in vitro if this antimicrobial activity was maintained using a novel canine skin explant model.</p><p><strong>Materials and methods: </strong>Punch biopsies (8 mm) of skin from recently euthanised dogs were collected and placed into six-well plates on top of an agarose pedestal.</p><p><strong>Results: </strong>Histological examination of the skin explants showed an intact dermal-epidermal organisation and a stratum corneum that was successfully colonised by S. pseudintermedius after topical application. The number of colony forming units of S. pseudintermedius showed a 2 log increase after 24 h colonisation, indicating that the explant supported bacterial growth. By contrast, co-treatment with S. felis C4 live bacteria and its sterile protein product significantly reduced the growth of a methicillin-susceptible (ST540, p = 0.0357) and a methicillin-resistant (MR) strain (ST71, p = 0.0143) of S. pseudintermedius. No detectable bacteria were recovered from or visualised on skin 24 h posttreatment with the S. felis C4 sterile protein product.</p><p><strong>Conclusions and clinical relevance: </strong>Using a novel canine explant model, we demonstrate that the S. felis C4 strain inhibits the growth of S. pseudintermedius and that it is a promising candidate for a new probiotic therapy to treat cutaneous infections caused by S. pseudintermedius, including MR strains.</p>\",\"PeriodicalId\":23599,\"journal\":{\"name\":\"Veterinary dermatology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-10-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary dermatology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/vde.13308\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary dermatology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/vde.13308","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:犬浅表性脓皮病是一种常见的犬皮肤细菌感染,通常由假中间葡萄球菌引起。最近发现猫葡萄球菌的 C4 菌株对小鼠体内的假中间葡萄球菌有很强的抗菌活性:我们旨在使用一种新型犬皮肤切片模型,在体外评估这种抗菌活性是否得以维持:材料: 收集最近安乐死的狗的皮肤切片(8 毫米),并将其放入琼脂糖底座上的六孔板中:结果:对皮肤切片的组织学检查显示,真皮-表皮组织完整,角质层在局部应用后被假中间体成功定殖。假性角膜炎双球菌的菌落形成单位数在定植 24 小时后增加了 2 个对数,表明外植体支持细菌生长。相比之下,与 S. felis C4 活菌及其无菌蛋白产物共同处理可显著减少假中间芽孢杆菌的甲氧西林敏感菌株(ST540,p = 0.0357)和甲氧西林耐药(MR)菌株(ST71,p = 0.0143)的生长。使用猫科动物裂头蚴 C4 无菌蛋白产品处理 24 小时后,皮肤上未发现可检测到的细菌:通过使用一种新型犬外植体模型,我们证明了 S. felis C4 菌株可抑制伪中间肠球菌的生长,是治疗由伪中间肠球菌(包括 MR 菌株)引起的皮肤感染的新型益生菌疗法的理想候选菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Staphylococcus felis C4 exhibits in vitro antimicrobial activity against methicillin-resistant Staphylococcus pseudintermedius in a novel canine skin explant model.

Background: Canine superficial pyoderma is a common bacterial skin infection of dogs, generally caused by Staphylococcus pseudintermedius. The C4 strain of Staphylococcus felis was recently discovered to have strong antimicrobial activity against S. pseudintermedius in mice.

Objectives: We aimed to evaluate in vitro if this antimicrobial activity was maintained using a novel canine skin explant model.

Materials and methods: Punch biopsies (8 mm) of skin from recently euthanised dogs were collected and placed into six-well plates on top of an agarose pedestal.

Results: Histological examination of the skin explants showed an intact dermal-epidermal organisation and a stratum corneum that was successfully colonised by S. pseudintermedius after topical application. The number of colony forming units of S. pseudintermedius showed a 2 log increase after 24 h colonisation, indicating that the explant supported bacterial growth. By contrast, co-treatment with S. felis C4 live bacteria and its sterile protein product significantly reduced the growth of a methicillin-susceptible (ST540, p = 0.0357) and a methicillin-resistant (MR) strain (ST71, p = 0.0143) of S. pseudintermedius. No detectable bacteria were recovered from or visualised on skin 24 h posttreatment with the S. felis C4 sterile protein product.

Conclusions and clinical relevance: Using a novel canine explant model, we demonstrate that the S. felis C4 strain inhibits the growth of S. pseudintermedius and that it is a promising candidate for a new probiotic therapy to treat cutaneous infections caused by S. pseudintermedius, including MR strains.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary dermatology
Veterinary dermatology 农林科学-兽医学
CiteScore
3.20
自引率
21.40%
发文量
92
审稿时长
12-24 weeks
期刊介绍: Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities
期刊最新文献
Long-lasting otic solution containing mometasone furoate can influence intradermal testing in dogs with healthy ears and otitis externa. Clinical features, surgical management and outcome of squamous and basal cell carcinoma in squamates and chelonians. Skin barrier, phenotypic and genotypic characterisation of autosomal recessive ichthyosis in TGM1-deficient Jack Russell Terriers and response to topical ceramide. Treatment of moderate-to-severe canine atopic dermatitis with modified-release mycophenolate (OKV-1001): A pilot open-label, single-arm multicentric clinical trial. Analysis of the immunomodulatory properties of mycobacterium cell wall fraction on the cytokine production of peripheral blood mononuclear cells of healthy dogs.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1