在家蚕感染模型中对远真菌菌丝形成的组织病理学分析

IF 1.9 Q3 PHARMACOLOGY & PHARMACY Drug Discoveries and Therapeutics Pub Date : 2024-11-15 Epub Date: 2024-10-24 DOI:10.5582/ddt.2024.01064
Koki Iwata, Mizuho Sato, Shoko Yoshida, Hiroo Wada, Kazuhisa Sekimizu, Mitsuhiro Okazaki
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引用次数: 0

摘要

家蚕诺卡氏菌感染模型已被确立为筛选诺卡氏菌致病性和评估抗菌剂对诺卡氏菌感染治疗效果的有用动物模型。目前还没有对感染了远心诺卡氏菌的家蚕进行组织病理学分析。在本研究中,我们对感染了远志野卡氏菌的家蚕器官进行了组织学分析。在感染远志蚕球虫一天后,血淋巴中的蚕球虫细胞形成了分支丝状。此外,我们在蚕感染后 30 秒和 24 小时内用阿米卡星处理受感染的蚕,评估了有效剂量(ED50)值,发现在感染后 30 秒和 24 小时内处理的 ED50 值分别为 4.1 μg/larva 和 5.6 μg/larva。用阿米卡星处理受感染家蚕的评估结果不受诺卡氏菌生长过程形式的影响。这些结果表明,家蚕诺卡氏菌感染模型是评估远华诺卡氏菌生长过程中抗菌治疗的有用工具。
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Histopathological analysis of filament formation of Nocardia farcinica in a silkworm infection model.

The silkworm Nocardia infection model has been established as a useful animal model for screening the pathogenicity of Nocardia and evaluating the therapeutic effects of antimicrobial agents against Nocardia infection. No histopathological analysis of silkworms infected with Nocardia farcinica has yet been performed. In this study, we performed histological analyses on organs of silkworms infected with N. farcinica. One day after infection with N. farcinica, the organism developed a branching filamentous form from coccid cells in the hemolymph. In addition, we evaluated effective doses (ED50) values by treating infected silkworms with amikacin 30 seconds and 24 hours after infection and found that the ED50 values treated within 30 seconds and 24 hours after infection were 4.1 μg/larva and 5.6 μg/larva, respectively. Evaluation of treatment with amikacin against the infected silkworms was unaffected by the growth process form of Nocardia. These results suggest that the silkworm Nocardia infection model is a useful tool for evaluating the antimicrobial therapy in the growth process of the N. farcinica.

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来源期刊
Drug Discoveries and Therapeutics
Drug Discoveries and Therapeutics PHARMACOLOGY & PHARMACY-
CiteScore
3.20
自引率
3.20%
发文量
51
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